EC Number |
Protein Variants |
Reference |
---|
2.7.1.145 | A110D |
site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme |
661583 |
2.7.1.145 | E52D |
markedly decreased kcat |
673584 |
2.7.1.145 | E52H |
markedly decreased kcat |
673584 |
2.7.1.145 | E52Q |
inactive mutant protein |
673584 |
2.7.1.145 | F114A |
site-directed mutagenesis, active site mutant, altered substrate specificity compared to the wild-type enzyme |
661629 |
2.7.1.145 | F114Y |
site-directed mutagenesis, active site mutant, altered substrate specificity compared to the wild-type enzyme |
661629 |
2.7.1.145 | I102M/N117S/M118V/D208N |
clones harbouring the mutant enzyme are more sensitive to 3'-azido-2',3'-dideoxythymidine and beta-D-arabinofuranosylcytosine |
636844 |
2.7.1.145 | I29H |
site-directed mutagenesis, active site mutant, altered substrate specificity compared to the wild-type enzyme |
661629 |
2.7.1.145 | I29H/F114Y |
site-directed mutagenesis, active site mutant, inactive mutant |
661629 |
2.7.1.145 | I47T/N210D |
clones harbouring the mutant enzyme are more sensitive to 3'-azido-2',3'-dideoxythymidine and beta-D-arabinofuranosylcytosine |
636844 |