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EC Number Protein Variants Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 2.6.1.B21H86F site-directed mutagenesis, the mutant shows no activity with (R)-PEA and no wild-type DATA activity 758564
Display the word mapDisplay the reaction diagram Show all sequences 2.6.1.B21more creation of an (R)-amine transaminase activity within an alpha-amino acid transaminase scaffold via one to six amino acid substitutions in the enzyme's active site. The final sextuple variant M2-6 shows almost complete depletion of the native DATA activity but high activity toward (R)-phenylethylamine ((R)-PEA) and has a high specific activity in the conversion of (R)-PEA and pyruvate to acetophenone and D-alanine. The crystal structure of the D-amino acid aminotransferase (DATA, UniProt ID P19938, EC 2.6.1.21) from Bacillus sp. strain YM-1 (PDB entry 3DAA) is used as a scaffold for the computational enzyme redesign. Molecular dynamics simulations and molecular mechanics Poisson-Boltzmann surface area (MM/PBSA) calculations 758564
Display the word mapDisplay the reaction diagram Show all sequences 2.6.1.B21S180A site-directed mutagenesis, the mutant shows no activity with (R)-PEA and no wild-type DATA activity 758564
Display the word mapDisplay the reaction diagram Show all sequences 2.6.1.B21Y31F site-directed mutagenesis, the mutant shows low activity with (R)-PEA and good wild-type DATA activity 758564
Display the word mapDisplay the reaction diagram Show all sequences 2.6.1.B21Y31F/H86F site-directed mutagenesis, variant M2 exhibited some (R)-PEA acceptance when PMP formation after incubating the purified variant with (R)-PEA is assayed, which indicates a completed first half-reaction. Lack of activity in the acetophenone assay 758564
Display the word mapDisplay the reaction diagram Show all sequences 2.6.1.B21Y31F/H86F/S180A/T242I site-directed mutagenesis, variant M2-4 shows significant activity toward (R)-PEA, and retains no native DATA activity 758564
Display the word mapDisplay the reaction diagram Show all sequences 2.6.1.B21Y31F/H86F/Y88F site-directed mutagenesis, variant M2-3 shows significant activity toward (R)-PEA and retains 34% of the native DATA activity 758564
Display the word mapDisplay the reaction diagram Show all sequences 2.6.1.B21Y31F/H86F/Y88F/H100L/S180A/T242I site-directed mutagenesis, variant M2-6 shows almost complete depletion of the native DATA activity but high activity toward (R)-phenylethylamine ((R)-PEA) and has a specific activity of 326 milliunits/mg in the conversion of (R)-PEA and pyruvate to acetophenone and D-alanine 758564
Display the word mapDisplay the reaction diagram Show all sequences 2.6.1.B21Y31F/H86F/Y88F/S180A/T242I site-directed mutagenesis, variant M2-5 shows significant activity toward (R)-PEA and partially retains native DATA activity 758564
Display the word mapDisplay the reaction diagram Show all sequences 2.6.1.B21Y88E site-directed mutagenesis, the mutant shows no activity with (R)-PEA and no wild-type DATA activity 758564
Results 1 - 10 of 23 > >>
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