Any feedback?
No. of entries
Information on EC 2.6.1.B21 - (R)-amine:2-oxo acid transaminase
for references in articles please use BRENDA:EC2.6.1.B21
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
preliminary BRENDA-supplied EC number
EC Tree 2 Transferases
2.6 Transferring nitrogenous groups
2.6.1 Transaminases
2.6.1.B21 (R)-amine:2-oxo acid transaminase
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
- 2.6.1.B21
-
r-selective
- transaminases
- aspergillus
-
r-amine
- synthesis
- ketone
-
pyridoxal-5'-phosphate-dependent
-
b-factors
- fumigatus
-
enantioselectivity
-
transamination
- aminotransferases
- d-alanine
-
branched-chain
-
toolbox
-
phasing
-
arthrobacter
-
furtherly
-
stereoselective
showSynonyms
(r)-selective amine transaminase, (r)-amine transaminase, (r)-transaminase, (r)-selective aminotransferase, (r)-ata, more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
(R)-amine:pyruvate transaminase
(R)-omega-TA
(R)-selective (R)-amine:pyruvate transaminase
(R)-selective aminotransferase
(R)-selective omega-aminotransferase
(R)-selective omega-TA
Hoch3033
IlvE
omega-transaminase
PabC
R-omegaAT
R-omegaAT_Bcel
R-omegaAT_Sery
additional information
additional information
see also branched-chain amino acid transaminase, EC 2.6.1.42
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
(R)-alpha-methylbenzylamine + 2-oxo acid = acetophenone + L-amino acid
-
-
-
-
(R)-alpha-methylbenzylamine + 2-oxo acid = acetophenone + L-amino acid
first transaminase half-reaction via internal aldimine, external aldimine, ketimine, and pyridoxamine (PMP)-bound form
(R)-alpha-methylbenzylamine + 2-oxo acid = acetophenone + L-amino acid
first transaminase half-reaction via internal aldimine, external aldimine, ketimine, and pyridoxamine (PMP)-bound form
-
-
(R)-alpha-methylbenzylamine + 2-oxo acid = acetophenone + L-amino acid
first transaminase half-reaction via internal aldimine, external aldimine, ketimine, and pyridoxamine (PMP)-bound form
-
-
(R)-alpha-methylbenzylamine + 2-oxo acid = acetophenone + L-amino acid
first transaminase half-reaction via internal aldimine, external aldimine, ketimine, and pyridoxamine (PMP)-bound form
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
(R)-amine:2-oxo acid aminotransferase
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(R)-1-phenylethylamine + 2-oxoisocaproate
acetophenone + D-leucine
-
Substrates: -
Products: -
Products: -
r
(R)-1-phenylethylamine + pyruvate
acetophenone + D-alanine
-
Substrates: -
Products: -
Products: -
r
(R)-alpha-ethylbenzylamine + 3-methyl-2-oxovalerate
alpha-ethylbenzaldehyde + L-isoleucine
Substrates: reaction of (R)-amine:pyruvate transaminase, EC 2.6.1.B21
Products: -
Products: -
r
(R)-alpha-ethylbenzylamine + pyruvate
alpha-ethylbenzaldehyde + D-alanine
Substrates: R-EtBA
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + 2-oxobutyrate
acetophenone + L-alpha-aminobutyric acid
Substrates: -
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + 2-oxohexanoate
acetophenone + L-norleucine
Substrates: -
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + 2-oxovalerate
acetophenone + L-norvaline
Substrates: -
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + 3-methyl-2-oxobutyrate
acetophenone + L-valine
Substrates: -
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + 3-methyl-2-oxovalerate
acetophenone + D-isoleucine
Substrates: -
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + 4-methyl-2-oxovalerate
acetophenone + L-leucine
Substrates: -
Products: -
Products: -
r
2,2-dimethyl-1-phenyl-propan-1-one + isopropylamine
(R)-2,2-dimethyl-1-phenylpropan-1-amine + acetone
-
Substrates: the conversion by all active enzyme mutants lays between 22 and 71% with isopropylamine as amine donor, with enantiomeric excess above 99%, low activity
Products: -
Products: -
r
2,2-dimethyl-1-phenyl-propan-1-one + L-alanine
(R)-2,2-dimethyl-1-phenylpropan-1-amine + pyruvate
-
Substrates: the conversion by all active enzyme mutants is complete with L-alanine as amine donor, with enantiomeric excess above 99%
Products: -
Products: -
r
2-oxo-4-hydroxybutyrate + isopropylamine
D-homoserine + acetone
-
Substrates: low activity, 6% activity compared to pyruvate
Products: -
Products: -
r
2-oxo-hexanoate + isopropylamine
D-norleucine + acetone
-
Substrates: low activity, 2% activity compared to pyruvate
Products: -
Products: -
r
2-oxobutyrate + isopropylamine
D-2-aminobutyrate + acetone
-
Substrates: 33% activity compared to pyruvate
Products: -
Products: -
r
2-oxohexanoate + L-glutamate
norleucine + 2-oxoglutarate
Substrates: reaction of branched-chain amino acid transaminase, EC 2.6.1.42
Products: -
Products: -
r
2-oxovalerate + isopropylamine
D-2-amino-pentanoate + acetone
-
Substrates: low activity, 2% activity with 2b compared to pyruvate
Products: -
Products: -
r
L-alanine + 2-oxoglutarate
pyruvate + L-glutamate
Substrates: reaction of branched-chain amino acid transaminase, EC 2.6.1.42
Products: -
Products: -
r
L-isoleucine + 2-oxoglutarate
3-methyl-2-oxopentanoate + L-glutamate
Substrates: reaction of branched-chain amino acid transaminase, EC 2.6.1.42
Products: -
Products: -
r
L-valine + 2-oxoglutarate
3-methyl-2-oxobutanoate + L-glutamate
Substrates: reaction of branched-chain amino acid transaminase, EC 2.6.1.42
Products: -
Products: -
r
norvaline + 2-oxoglutarate
2-oxovalerate + L-glutamate
Substrates: reaction of branched-chain amino acid transaminase, EC 2.6.1.42
Products: -
Products: -
r
rac-2,2-dimethyl-1-phenylpropan-1-amine + pyruvate
2,2-dimethyl-1-phenyl-propan-1-one + L-alanine
-
Substrates: -
Products: -
Products: -
r
(R)-1-phenylethylamine + 3-methyl-2-oxovalerate
acetophenone + L-isoleucine
Substrates: the enzyme is active towards (R)-PEA and not active towards (S)-PEA
Products: -
Products: -
r
(R)-1-phenylethylamine + 3-methyl-2-oxovalerate
acetophenone + L-isoleucine
Substrates: the enzyme is active towards (R)-PEA and not active towards (S)-PEA
Products: -
Products: -
r
(R)-1-phenylethylamine + 3-methyl-2-oxovalerate
acetophenone + L-isoleucine
Substrates: the enzyme is active towards (R)-PEA and not active towards (S)-PEA
Products: -
Products: -
r
(R)-1-phenylethylamine + 3-methyl-2-oxovalerate
acetophenone + L-isoleucine
Substrates: the enzyme is active towards (R)-PEA and not active towards (S)-PEA
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + 2-oxoglutarate
acetophenone + D-glutamate
Substrates: -
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + 2-oxoglutarate
acetophenone + D-glutamate
Substrates: very low activity
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + 2-oxoglutarate
acetophenone + D-glutamate
Substrates: -
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + 2-oxoglutarate
acetophenone + D-glutamate
Substrates: very low activity
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + 2-oxoglutarate
acetophenone + D-glutamate
Evansella cellulosilytica ATCC 21833
Substrates: -
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + 2-oxoglutarate
acetophenone + D-glutamate
Substrates: -
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + 2-oxoglutarate
acetophenone + D-glutamate
Evansella cellulosilytica FERM P-1141
Substrates: -
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + 2-oxoglutarate
acetophenone + D-glutamate
Evansella cellulosilytica JCM 9156
Substrates: -
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + 2-oxoglutarate
acetophenone + D-glutamate
Substrates: -
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + 2-oxoglutarate
acetophenone + D-glutamate
Substrates: -
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + 2-oxoglutarate
acetophenone + D-glutamate
Substrates: low activity
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + 2-oxoglutarate
acetophenone + D-glutamate
Substrates: low activity
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + 2-oxoglutarate
acetophenone + D-glutamate
Substrates: low activity
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + 2-oxoglutarate
acetophenone + D-glutamate
Substrates: low activity
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + 2-oxoglutarate
acetophenone + D-glutamate
Substrates: low activity
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + 2-oxoglutarate
acetophenone + D-glutamate
Substrates: low activity
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + 2-oxoglutarate
acetophenone + D-glutamate
Substrates: low activity
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + 2-oxoglutarate
acetophenone + L-glutamate
Substrates: -
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + 2-oxoglutarate
acetophenone + L-glutamate
Substrates: reaction of (R)-amine:pyruvate transaminase, EC 2.6.1.B21
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + 3-methyl-2-oxovalerate
acetophenone + L-isoleucine
Substrates: -
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + 3-methyl-2-oxovalerate
acetophenone + L-isoleucine
Substrates: reaction of (R)-amine:pyruvate transaminase, EC 2.6.1.B21
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + pyruvate
acetophenone + D-alanine
Substrates: -
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + pyruvate
acetophenone + D-alanine
Substrates: the enzyme prefers pyruvate as the amino acceptor
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + pyruvate
acetophenone + D-alanine
Substrates: -
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + pyruvate
acetophenone + D-alanine
Substrates: the enzyme prefers pyruvate as the amino acceptor
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + pyruvate
acetophenone + D-alanine
Evansella cellulosilytica ATCC 21833
Substrates: -
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + pyruvate
acetophenone + D-alanine
Substrates: -
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + pyruvate
acetophenone + D-alanine
Evansella cellulosilytica FERM P-1141
Substrates: -
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + pyruvate
acetophenone + D-alanine
Evansella cellulosilytica JCM 9156
Substrates: -
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + pyruvate
acetophenone + D-alanine
Substrates: -
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + pyruvate
acetophenone + D-alanine
Substrates: R-MBA, specific substrate for (R)-amine:pyruvate transaminases
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + pyruvate
acetophenone + D-alanine
Substrates: -
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + pyruvate
acetophenone + D-alanine
Substrates: -
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + pyruvate
acetophenone + D-alanine
Substrates: -
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + pyruvate
acetophenone + D-alanine
Substrates: -
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + pyruvate
acetophenone + D-alanine
Substrates: -
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + pyruvate
acetophenone + D-alanine
Substrates: -
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + pyruvate
acetophenone + D-alanine
Substrates: -
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + pyruvate
acetophenone + L-alanine
-
Substrates: -
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + pyruvate
acetophenone + L-alanine
-
Substrates: isopropylamine is one of the preferred amino donors for omega-TA
Products: -
Products: -
r
D-alanine + 2-oxoglutarate
pyruvate + D-glutamate
Substrates: -
Products: -
Products: -
r
D-alanine + 2-oxoglutarate
pyruvate + D-glutamate
Substrates: very low activity, reaction of EC 2.6.1.21
Products: -
Products: -
r
D-alanine + 2-oxoglutarate
pyruvate + D-glutamate
Substrates: very low activity, reaction of EC 2.6.1.21
Products: -
Products: -
r
D-alanine + 2-oxoglutarate
pyruvate + D-glutamate
Substrates: very low activity, reaction of EC 2.6.1.21
Products: -
Products: -
r
D-alanine + 2-oxoglutarate
pyruvate + D-glutamate
Substrates: very low activity, reaction of EC 2.6.1.21
Products: -
Products: -
r
D-alanine + 2-oxoglutarate
pyruvate + D-glutamate
Substrates: very low activity, reaction of EC 2.6.1.21
Products: -
Products: -
r
D-alanine + 2-oxoglutarate
pyruvate + D-glutamate
Substrates: very low activity, reaction of EC 2.6.1.21
Products: -
Products: -
r
D-alanine + 2-oxoglutarate
pyruvate + D-glutamate
Substrates: very low activity, reaction of EC 2.6.1.21
Products: -
Products: -
r
L-leucine + 2-oxoglutarate
4-methyl-2-oxopentanoate + D-glutamate
Substrates: reaction of EC 2.6.1.42
Products: -
Products: -
r
L-leucine + 2-oxoglutarate
4-methyl-2-oxopentanoate + D-glutamate
Substrates: reaction of EC 2.6.1.42
Products: -
Products: -
r
L-leucine + 2-oxoglutarate
4-methyl-2-oxopentanoate + D-glutamate
Evansella cellulosilytica ATCC 21833
Substrates: reaction of EC 2.6.1.42
Products: -
Products: -
r
L-leucine + 2-oxoglutarate
4-methyl-2-oxopentanoate + D-glutamate
Substrates: reaction of EC 2.6.1.42
Products: -
Products: -
r
L-leucine + 2-oxoglutarate
4-methyl-2-oxopentanoate + D-glutamate
Evansella cellulosilytica FERM P-1141
Substrates: reaction of EC 2.6.1.42
Products: -
Products: -
r
L-leucine + 2-oxoglutarate
4-methyl-2-oxopentanoate + D-glutamate
Evansella cellulosilytica JCM 9156
Substrates: reaction of EC 2.6.1.42
Products: -
Products: -
r
L-leucine + 2-oxoglutarate
4-methyl-2-oxopentanoate + D-glutamate
Substrates: reaction of EC 2.6.1.42
Products: -
Products: -
r
L-leucine + 2-oxoglutarate
4-methyl-2-oxopentanoate + L-glutamate
Substrates: reaction of EC 2.6.1.42
Products: -
Products: -
r
L-leucine + 2-oxoglutarate
4-methyl-2-oxopentanoate + L-glutamate
Substrates: reaction of EC 2.6.1.42
Products: -
Products: -
r
L-leucine + 2-oxoglutarate
4-methyl-2-oxopentanoate + L-glutamate
Substrates: reaction of branched-chain amino acid transaminase, EC 2.6.1.42
Products: -
Products: -
r
additional information
?
-
-
Substrates: developed of a deracemization method for production of D-amino acids using L-alanine dehydrogenase (AlaDH), D-selective omega-transaminase (omega-TA) ARTAmut, and NADH oxidase (NOX). Unwanted L-amino acid in the racemic mixture is converted to a D-form after two consecutive reactions catalyzed by AlaDH and ARTA mut, leading to complete deracemization or enantioenrichment depending on the substrate specificity of the two enzymes, reaction cascade and method evaluation, overview
Products: -
Products: -
-
additional information
?
-
Substrates: (R)-amination mediated by (R)-specific omega-transaminases generally requires costly D-alanine in excess to obtain the desired chiral amines in high yield. A one-pot, trienzymatic cascade comprising an (R)-specific omega-transaminase, an amine dehydrogenase, and a formate dehydrogenase is developed for the economical and ecofriendly synthesis of (R)-chiral amines. Using inexpensive ammonium formate as the sole sacrificial agent, the established cascade system enables efficient omega-transaminase-mediated (R)-amination of various ketones, with high conversions and excellent enantiomeric excess (over 99%), water and CO2 are the only waste products. The wild-type enzyme shows broad substrate specificity and enantioselectivity. Cascade reaction, overview
Products: -
Products: -
-
additional information
?
-
Substrates: (R)-amination mediated by (R)-specific omega-transaminases generally requires costly D-alanine in excess to obtain the desired chiral amines in high yield. A one-pot, trienzymatic cascade comprising an (R)-specific omega-transaminase, an amine dehydrogenase, and a formate dehydrogenase is developed for the economical and ecofriendly synthesis of (R)-chiral amines. Using inexpensive ammonium formate as the sole sacrificial agent, the established cascade system enables efficient omega-transaminase-mediated (R)-amination of various ketones, with high conversions and excellent enantiomeric excess (over 99%), water and CO2 are the only waste products. The wild-type enzyme shows broad substrate specificity and enantioselectivity. Cascade reaction, overview
Products: -
Products: -
-
additional information
?
-
Substrates: (R)-amination mediated by (R)-specific omega-transaminases generally requires costly D-alanine in excess to obtain the desired chiral amines in high yield. A one-pot, trienzymatic cascade comprising an (R)-specific omega-transaminase, an amine dehydrogenase, and a formate dehydrogenase is developed for the economical and ecofriendly synthesis of (R)-chiral amines. Using inexpensive ammonium formate as the sole sacrificial agent, the established cascade system enables efficient omega-transaminase-mediated (R)-amination of various ketones, with high conversions and excellent enantiomeric excess (over 99%), water and CO2 are the only waste products. The wild-type enzyme shows broad substrate specificity and enantioselectivity. Cascade reaction, overview
Products: -
Products: -
-
additional information
?
-
-
Substrates: besides R-omegaAT activity, the enzyme R-omegaAT_Bthu also possesses BCAT (EC 2.6.1.42) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. Enzyme R-omegaAT_Bthu shows very low R-omegaAT activity. The enzyme does not show any D-alanine aminotransferase (DAT, EC 2.6.1.21) activity
Products: -
Products: -
-
additional information
?
-
Substrates: besides R-omegaAT activity, the enzyme R-omegaAT_Bthu also possesses BCAT (EC 2.6.1.42) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. Enzyme R-omegaAT_Bthu shows very low R-omegaAT activity. The enzyme does not show any D-alanine aminotransferase (DAT, EC 2.6.1.21) activity
Products: -
Products: -
-
additional information
?
-
-
Substrates: the forward reaction is preferred. Enzyme R-omegaAT_Bthu mainly possesses BCAT activity (EC 2.6.1.42). No activity with (S)-alpha-methylbenzylamine and D-alanine
Products: -
Products: -
-
additional information
?
-
Substrates: the forward reaction is preferred. Enzyme R-omegaAT_Bthu mainly possesses BCAT activity (EC 2.6.1.42). No activity with (S)-alpha-methylbenzylamine and D-alanine
Products: -
Products: -
-
additional information
?
-
-
Substrates: besides R-omegaAT activity, the enzyme R-omegaAT_Bcel also possesses BCAT (EC 2.6.1.42) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. Enzyme R-omegaAT_Bcel shows very low R-omegaAT activity. The enzyme does not show any D-alanine aminotransferase (DAT, EC 2.6.1.21) activity
Products: -
Products: -
-
additional information
?
-
Substrates: besides R-omegaAT activity, the enzyme R-omegaAT_Bcel also possesses BCAT (EC 2.6.1.42) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. Enzyme R-omegaAT_Bcel shows very low R-omegaAT activity. The enzyme does not show any D-alanine aminotransferase (DAT, EC 2.6.1.21) activity
Products: -
Products: -
-
additional information
?
-
-
Substrates: the forward reaction is preferred. Enzyme R-omegaAT_Sery mainly possesses BCAT activity (EC 2.6.1.42). No activity with (S)-alpha-methylbenzylamine and D-alanine
Products: -
Products: -
-
additional information
?
-
Substrates: the forward reaction is preferred. Enzyme R-omegaAT_Sery mainly possesses BCAT activity (EC 2.6.1.42). No activity with (S)-alpha-methylbenzylamine and D-alanine
Products: -
Products: -
-
additional information
?
-
Evansella cellulosilytica ATCC 21833
-
Substrates: the forward reaction is preferred. Enzyme R-omegaAT_Sery mainly possesses BCAT activity (EC 2.6.1.42). No activity with (S)-alpha-methylbenzylamine and D-alanine
Products: -
Products: -
-
additional information
?
-
Evansella cellulosilytica ATCC 21833
Substrates: the forward reaction is preferred. Enzyme R-omegaAT_Sery mainly possesses BCAT activity (EC 2.6.1.42). No activity with (S)-alpha-methylbenzylamine and D-alanine
Products: -
Products: -
-
additional information
?
-
Evansella cellulosilytica ATCC 21833
-
Substrates: besides R-omegaAT activity, the enzyme R-omegaAT_Bcel also possesses BCAT (EC 2.6.1.42) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. Enzyme R-omegaAT_Bcel shows very low R-omegaAT activity. The enzyme does not show any D-alanine aminotransferase (DAT, EC 2.6.1.21) activity
Products: -
Products: -
-
additional information
?
-
Evansella cellulosilytica ATCC 21833
Substrates: besides R-omegaAT activity, the enzyme R-omegaAT_Bcel also possesses BCAT (EC 2.6.1.42) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. Enzyme R-omegaAT_Bcel shows very low R-omegaAT activity. The enzyme does not show any D-alanine aminotransferase (DAT, EC 2.6.1.21) activity
Products: -
Products: -
-
additional information
?
-
-
Substrates: the forward reaction is preferred. Enzyme R-omegaAT_Sery mainly possesses BCAT activity (EC 2.6.1.42). No activity with (S)-alpha-methylbenzylamine and D-alanine
Products: -
Products: -
-
additional information
?
-
Substrates: the forward reaction is preferred. Enzyme R-omegaAT_Sery mainly possesses BCAT activity (EC 2.6.1.42). No activity with (S)-alpha-methylbenzylamine and D-alanine
Products: -
Products: -
-
additional information
?
-
-
Substrates: besides R-omegaAT activity, the enzyme R-omegaAT_Bcel also possesses BCAT (EC 2.6.1.42) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. Enzyme R-omegaAT_Bcel shows very low R-omegaAT activity. The enzyme does not show any D-alanine aminotransferase (DAT, EC 2.6.1.21) activity
Products: -
Products: -
-
additional information
?
-
Substrates: besides R-omegaAT activity, the enzyme R-omegaAT_Bcel also possesses BCAT (EC 2.6.1.42) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. Enzyme R-omegaAT_Bcel shows very low R-omegaAT activity. The enzyme does not show any D-alanine aminotransferase (DAT, EC 2.6.1.21) activity
Products: -
Products: -
-
additional information
?
-
Evansella cellulosilytica FERM P-1141
-
Substrates: the forward reaction is preferred. Enzyme R-omegaAT_Sery mainly possesses BCAT activity (EC 2.6.1.42). No activity with (S)-alpha-methylbenzylamine and D-alanine
Products: -
Products: -
-
additional information
?
-
Evansella cellulosilytica FERM P-1141
Substrates: the forward reaction is preferred. Enzyme R-omegaAT_Sery mainly possesses BCAT activity (EC 2.6.1.42). No activity with (S)-alpha-methylbenzylamine and D-alanine
Products: -
Products: -
-
additional information
?
-
Evansella cellulosilytica FERM P-1141
-
Substrates: besides R-omegaAT activity, the enzyme R-omegaAT_Bcel also possesses BCAT (EC 2.6.1.42) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. Enzyme R-omegaAT_Bcel shows very low R-omegaAT activity. The enzyme does not show any D-alanine aminotransferase (DAT, EC 2.6.1.21) activity
Products: -
Products: -
-
additional information
?
-
Evansella cellulosilytica FERM P-1141
Substrates: besides R-omegaAT activity, the enzyme R-omegaAT_Bcel also possesses BCAT (EC 2.6.1.42) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. Enzyme R-omegaAT_Bcel shows very low R-omegaAT activity. The enzyme does not show any D-alanine aminotransferase (DAT, EC 2.6.1.21) activity
Products: -
Products: -
-
additional information
?
-
Evansella cellulosilytica JCM 9156
-
Substrates: the forward reaction is preferred. Enzyme R-omegaAT_Sery mainly possesses BCAT activity (EC 2.6.1.42). No activity with (S)-alpha-methylbenzylamine and D-alanine
Products: -
Products: -
-
additional information
?
-
Evansella cellulosilytica JCM 9156
Substrates: the forward reaction is preferred. Enzyme R-omegaAT_Sery mainly possesses BCAT activity (EC 2.6.1.42). No activity with (S)-alpha-methylbenzylamine and D-alanine
Products: -
Products: -
-
additional information
?
-
Evansella cellulosilytica JCM 9156
-
Substrates: besides R-omegaAT activity, the enzyme R-omegaAT_Bcel also possesses BCAT (EC 2.6.1.42) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. Enzyme R-omegaAT_Bcel shows very low R-omegaAT activity. The enzyme does not show any D-alanine aminotransferase (DAT, EC 2.6.1.21) activity
Products: -
Products: -
-
additional information
?
-
Evansella cellulosilytica JCM 9156
Substrates: besides R-omegaAT activity, the enzyme R-omegaAT_Bcel also possesses BCAT (EC 2.6.1.42) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. Enzyme R-omegaAT_Bcel shows very low R-omegaAT activity. The enzyme does not show any D-alanine aminotransferase (DAT, EC 2.6.1.21) activity
Products: -
Products: -
-
additional information
?
-
-
Substrates: the forward reaction is preferred. Enzyme R-omegaAT_Sery mainly possesses BCAT activity (EC 2.6.1.42). No activity with (S)-alpha-methylbenzylamine and D-alanine
Products: -
Products: -
-
additional information
?
-
Substrates: the forward reaction is preferred. Enzyme R-omegaAT_Sery mainly possesses BCAT activity (EC 2.6.1.42). No activity with (S)-alpha-methylbenzylamine and D-alanine
Products: -
Products: -
-
additional information
?
-
-
Substrates: besides R-omegaAT activity, the enzyme R-omegaAT_Bcel also possesses BCAT (EC 2.6.1.42) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. Enzyme R-omegaAT_Bcel shows very low R-omegaAT activity. The enzyme does not show any D-alanine aminotransferase (DAT, EC 2.6.1.21) activity
Products: -
Products: -
-
additional information
?
-
Substrates: besides R-omegaAT activity, the enzyme R-omegaAT_Bcel also possesses BCAT (EC 2.6.1.42) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. Enzyme R-omegaAT_Bcel shows very low R-omegaAT activity. The enzyme does not show any D-alanine aminotransferase (DAT, EC 2.6.1.21) activity
Products: -
Products: -
-
additional information
?
-
-
Substrates: enzyme Hoch3033 is a transaminases with a n enzyme with mixed type of activity showing branched-chain amino acid amintransferase activity (EC 2.6.1.42) and (R)-amine:pyruvate transaminase (EC 2.6.1). The mixed type of activity of Hoch3033 is implemented within the BCAT-like active site, but in the active site of Hoch3033, substitutions of specificity-determining residues, that are important for substrate binding in canonical BCATs, are observed. In the standard assay, the specific activity towards (S)-alpha-methylbenzylamine (S-MBA) is only 0.32% of the activity towards R-MBA. A much lower value of activity is obtained with (R)-alpha-ethylbenzylamine (R-EtBA), the closest homologue of R-MBA. No activity towards S-EtBA is observed. Substrate specificity overview
Products: -
Products: -
-
additional information
?
-
Substrates: enzyme Hoch3033 is a transaminases with a n enzyme with mixed type of activity showing branched-chain amino acid amintransferase activity (EC 2.6.1.42) and (R)-amine:pyruvate transaminase (EC 2.6.1). The mixed type of activity of Hoch3033 is implemented within the BCAT-like active site, but in the active site of Hoch3033, substitutions of specificity-determining residues, that are important for substrate binding in canonical BCATs, are observed. In the standard assay, the specific activity towards (S)-alpha-methylbenzylamine (S-MBA) is only 0.32% of the activity towards R-MBA. A much lower value of activity is obtained with (R)-alpha-ethylbenzylamine (R-EtBA), the closest homologue of R-MBA. No activity towards S-EtBA is observed. Substrate specificity overview
Products: -
Products: -
-
additional information
?
-
Substrates: no activity with 2-oxoglutarate and with beta-Ala and D-Ala. The deamination of L-Leu and L-Ile by the transaminase from Haliangium ochraceum is observed only in half-reactions. Reaction mechanisms
Products: -
Products: -
-
additional information
?
-
-
Substrates: the bifunctional transaminase from myxobacterium Haliangium ochraceum, encoded by gene Hoch3033, is active towards keto analogues of branched-chain amino acids (specific substrates for BCATs, EC 2.6.1.42) and (R)-alpha-methylbenzylamine (specific substrate for (R)-amine:pyruvate transaminases, EC 2.6.1.B21). The enzyme shows no activity with (S)-2-methylbenzylamine, 2-amino-5-methylhexane, 1-methyl-3-phenyl-propylamine, (R)-2-aminohexane, D-alanine, L-beta-leucine, and beta-alanine
Products: -
Products: -
-
additional information
?
-
Substrates: the bifunctional transaminase from myxobacterium Haliangium ochraceum, encoded by gene Hoch3033, is active towards keto analogues of branched-chain amino acids (specific substrates for BCATs, EC 2.6.1.42) and (R)-alpha-methylbenzylamine (specific substrate for (R)-amine:pyruvate transaminases, EC 2.6.1.B21). The enzyme shows no activity with (S)-2-methylbenzylamine, 2-amino-5-methylhexane, 1-methyl-3-phenyl-propylamine, (R)-2-aminohexane, D-alanine, L-beta-leucine, and beta-alanine
Products: -
Products: -
-
additional information
?
-
Substrates: no activity with 2-oxoglutarate and with beta-Ala and D-Ala. The deamination of L-Leu and L-Ile by the transaminase from Haliangium ochraceum is observed only in half-reactions. Reaction mechanisms
Products: -
Products: -
-
additional information
?
-
Substrates: no activity with 2-oxoglutarate and with beta-Ala and D-Ala. The deamination of L-Leu and L-Ile by the transaminase from Haliangium ochraceum is observed only in half-reactions. Reaction mechanisms
Products: -
Products: -
-
additional information
?
-
Substrates: no activity with 2-oxoglutarate and with beta-Ala and D-Ala. The deamination of L-Leu and L-Ile by the transaminase from Haliangium ochraceum is observed only in half-reactions. Reaction mechanisms
Products: -
Products: -
-
additional information
?
-
-
Substrates: engineered ATAs perform asymmetric synthesis of the respective R-amine with high conversions by using either alanine or isopropylamine as amine donor. Asymmetric synthesis of (R)-2,2-dimethyl-1-phenylpropan-1-amine by amino group transfer to 2,2-dimethyl-1-phenyl-propan-1-one catalyzed by ATAs. Isopropylamine or alanine serve as the amine donors. Analysis of specific activities of Rsp-ATA mutant variants towards rac-amine 2,2-dimethyl-1-phenylpropan-1-amine. Enzyme-ligand interaction analysis, overview
Products: -
Products: -
-
additional information
?
-
-
Substrates: besides R-omegaAT activity, the enzyme R-omegaAT_Sery also possesses D-alanine aminotransferase (DAT, EC 2.6.1.21) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. No BCAT activity (EC 2.6.1.42) is observed for enzyme R-omegaAT_Sery, no activity with L-leucine
Products: -
Products: -
-
additional information
?
-
Substrates: besides R-omegaAT activity, the enzyme R-omegaAT_Sery also possesses D-alanine aminotransferase (DAT, EC 2.6.1.21) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. No BCAT activity (EC 2.6.1.42) is observed for enzyme R-omegaAT_Sery, no activity with L-leucine
Products: -
Products: -
-
additional information
?
-
-
Substrates: the forward reaction is preferred. Enzyme R-omegaAT_Sery mainly possesses DAT activity (EC 2.6.1.21). No activity with (S)-alpha-methylbenzylamine and L-leucine
Products: -
Products: -
-
additional information
?
-
Substrates: the forward reaction is preferred. Enzyme R-omegaAT_Sery mainly possesses DAT activity (EC 2.6.1.21). No activity with (S)-alpha-methylbenzylamine and L-leucine
Products: -
Products: -
-
additional information
?
-
Substrates: besides R-omegaAT activity, the enzyme R-omegaAT_Sery also possesses D-alanine aminotransferase (DAT, EC 2.6.1.21) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. No BCAT activity (EC 2.6.1.42) is observed for enzyme R-omegaAT_Sery, no activity with L-leucine
Products: -
Products: -
-
additional information
?
-
Substrates: besides R-omegaAT activity, the enzyme R-omegaAT_Sery also possesses D-alanine aminotransferase (DAT, EC 2.6.1.21) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. No BCAT activity (EC 2.6.1.42) is observed for enzyme R-omegaAT_Sery, no activity with L-leucine
Products: -
Products: -
-
additional information
?
-
Substrates: besides R-omegaAT activity, the enzyme R-omegaAT_Sery also possesses D-alanine aminotransferase (DAT, EC 2.6.1.21) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. No BCAT activity (EC 2.6.1.42) is observed for enzyme R-omegaAT_Sery, no activity with L-leucine
Products: -
Products: -
-
additional information
?
-
Substrates: besides R-omegaAT activity, the enzyme R-omegaAT_Sery also possesses D-alanine aminotransferase (DAT, EC 2.6.1.21) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. No BCAT activity (EC 2.6.1.42) is observed for enzyme R-omegaAT_Sery, no activity with L-leucine
Products: -
Products: -
-
additional information
?
-
Substrates: besides R-omegaAT activity, the enzyme R-omegaAT_Sery also possesses D-alanine aminotransferase (DAT, EC 2.6.1.21) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. No BCAT activity (EC 2.6.1.42) is observed for enzyme R-omegaAT_Sery, no activity with L-leucine
Products: -
Products: -
-
additional information
?
-
Substrates: besides R-omegaAT activity, the enzyme R-omegaAT_Sery also possesses D-alanine aminotransferase (DAT, EC 2.6.1.21) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. No BCAT activity (EC 2.6.1.42) is observed for enzyme R-omegaAT_Sery, no activity with L-leucine
Products: -
Products: -
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(R)-alpha-methylbenzylamine + pyruvate
acetophenone + L-alanine
-
Substrates: -
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + pyruvate
acetophenone + D-alanine
Substrates: -
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + pyruvate
acetophenone + D-alanine
Substrates: -
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + pyruvate
acetophenone + D-alanine
Evansella cellulosilytica ATCC 21833
Substrates: -
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + pyruvate
acetophenone + D-alanine
Substrates: -
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + pyruvate
acetophenone + D-alanine
Evansella cellulosilytica FERM P-1141
Substrates: -
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + pyruvate
acetophenone + D-alanine
Evansella cellulosilytica JCM 9156
Substrates: -
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + pyruvate
acetophenone + D-alanine
Substrates: -
Products: -
Products: -
r
(R)-alpha-methylbenzylamine + pyruvate
acetophenone + D-alanine
Substrates: -
Products: -
Products: -
r
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE