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EC Number Protein Variants Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 2.6.1.B21more creation of an (R)-amine transaminase activity within an alpha-amino acid transaminase scaffold via one to six amino acid substitutions in the enzyme's active site. The final sextuple variant M2-6 shows almost complete depletion of the native DATA activity but high activity toward (R)-phenylethylamine ((R)-PEA) and has a high specific activity in the conversion of (R)-PEA and pyruvate to acetophenone and D-alanine. The crystal structure of the D-amino acid aminotransferase (DATA, UniProt ID P19938, EC 2.6.1.21) from Bacillus sp. strain YM-1 (PDB entry 3DAA) is used as a scaffold for the computational enzyme redesign. Molecular dynamics simulations and molecular mechanics Poisson-Boltzmann surface area (MM/PBSA) calculations 758564
Display the word mapDisplay the reaction diagram Show all sequences 2.6.1.B21more mutational analysis of enzyme-substrate interactions and substrate specificity, overview. The evolved ATAs perform asymmetric synthesis of the respective R-amine with high conversions by using either alanine or isopropylamine 759050
Display the word mapDisplay the reaction diagram Show all sequences 2.6.1.B21H106Y/Y108R site-directed mutagenesis -, 758848
Display the word mapDisplay the reaction diagram Show all sequences 2.6.1.B21Y59W/Y87V/T231A site-directed mutagenesis 759050
Display the word mapDisplay the reaction diagram Show all sequences 2.6.1.B21Y59W/T231A site-directed mutagenesis, inactive mutant 759050
Display the word mapDisplay the reaction diagram Show all sequences 2.6.1.B21Y59W/Y87F/T231A site-directed mutagenesis, inactive mutant 759050
Display the word mapDisplay the reaction diagram Show all sequences 2.6.1.B21Y59W/Y87L/T231A site-directed mutagenesis, specific and enantioimeric conversion of 2,2-dimethyl-1-phenylpropan-1-amine compared to wild-type 759050
Display the word mapDisplay the reaction diagram Show all sequences 2.6.1.B21Y59W/Y87L/T231A/G429A site-directed mutagenesis, specific and enantioimeric conversion of 2,2-dimethyl-1-phenylpropan-1-amine compared to wild-type 759050
Display the word mapDisplay the reaction diagram Show all sequences 2.6.1.B21Y59W/Y87L/T231A/L382M site-directed mutagenesis, specific and enantioimeric conversion of 2,2-dimethyl-1-phenylpropan-1-amine compared to wild-type 759050
Display the word mapDisplay the reaction diagram Show all sequences 2.6.1.B21Y59W/Y87L/T231A/L382M/G429A site-directed mutagenesis, specific and enantioimeric conversion of 2,2-dimethyl-1-phenylpropan-1-amine compared to wild-type 759050
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