EC Number |
Protein Variants |
Reference |
---|
2.4.1.20 | A397V |
random, site-saturation, and site-directed mutagenesis, the mutant enzyme shows reduced activity with lactose compared to the wild-type enzyme |
706588 |
2.4.1.20 | A397V/T508A/A512T/D557N/N667T/G681S |
random, site-saturation, and site-directed mutagenesis, the mutant enzyme shows 3fold increased activity with lactose and 6fold decreased activity with cellobiose compared to the wild-type enzyme |
706588 |
2.4.1.20 | A423S |
mutation introduced to increase thermostability, mutant displays a half-life of 11.3 min at 70°C |
720434 |
2.4.1.20 | A512T |
random, site-saturation, and site-directed mutagenesis, the mutant enzyme shows similar activity with lactose compared to the wild-type enzyme |
706588 |
2.4.1.20 | A781K |
mutation introduced to increase thermostability, mutant displays a half-life of 15.3 min at 70°C |
720434 |
2.4.1.20 | C485A |
the mutant shows 43% of wild type activity with cellobiose. The mutant shows higher preference for D-glucosamine than the wild type enzyme |
735893 |
2.4.1.20 | D557N |
random, site-saturation, and site-directed mutagenesis, the mutant enzyme shows similar activity with lactose compared to the wild-type enzyme |
706588 |
2.4.1.20 | E649C |
mutant accepts methyl beta-D-glucoside and ethyl beta-D-glucoside as substrates |
719136 |
2.4.1.20 | E654A |
inactive |
735893 |
2.4.1.20 | E654D |
inactive |
735893 |