Protein Variants | Comment | Organism |
---|---|---|
E649C | mutant accepts methyl beta-D-glucoside and ethyl beta-D-glucoside as substrates | Cellulomonas uda |
N156D/N163D/T508I/E649G/N667A | mutant with very broad acceptor specificity | Cellulomonas uda |
T508I/E649C/N667A | triple mutation broadens acceptor specificity | Cellulomonas uda |
T508I/N667A | mutant accepts methyl beta-D-glucoside and ethyl beta-D-glucoside as substrates | Cellulomonas uda |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.8 | - |
cellobiose | mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C | Cellulomonas uda | |
17.2 | - |
D-glucose | mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C | Cellulomonas uda | |
28.9 | - |
D-glucose | mutant T508I/E649C/N667A, pH 6.6, 37°C | Cellulomonas uda | |
31.2 | - |
phenyl beta-D-glucoside | mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C | Cellulomonas uda | |
33.7 | - |
butyl beta-D-glucoside | mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C | Cellulomonas uda | |
56.6 | - |
methyl beta-D-glucoside | mutant T508I/E649C/N667A, pH 6.6, 37°C | Cellulomonas uda | |
79.8 | - |
methyl beta-D-glucoside | mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C | Cellulomonas uda | |
138 | - |
ethyl beta-D-glucoside | mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C | Cellulomonas uda | |
155 | - |
ethyl beta-D-glucoside | mutant T508I/E649C/N667A, pH 6.6, 37°C | Cellulomonas uda | |
1009 | - |
methyl alpha-D-glucoside | mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C | Cellulomonas uda |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cellulomonas uda | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-glucose 1-phosphate + butyl beta-D-glucoside | no substrate for wild-type | Cellulomonas uda | ? + phosphate | - |
r | |
alpha-D-glucose 1-phosphate + cellobiose | no substrate for wilalpha-D-type | Cellulomonas uda | ? + phosphate | - |
r | |
alpha-D-glucose 1-phosphate + D-glucose | - |
Cellulomonas uda | ? + phosphate | - |
r | |
alpha-D-glucose 1-phosphate + ethyl beta-D-glucoside | no substrate for wild-type | Cellulomonas uda | ? + phosphate | - |
r | |
alpha-D-glucose 1-phosphate + methyl alpha-D-glucoside | no substrate for wild-type | Cellulomonas uda | ? + phosphate | - |
r | |
alpha-D-glucose 1-phosphate + methyl beta-D-glucoside | no substrate for wild-type | Cellulomonas uda | ? + phosphate | - |
r | |
alpha-D-glucose 1-phosphate + phenyl beta-D-glucoside | no substrate for wild-type | Cellulomonas uda | ? + phosphate | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.6 | - |
cellobiose | mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C | Cellulomonas uda | |
4 | - |
phenyl beta-D-glucoside | mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C | Cellulomonas uda | |
5.1 | - |
ethyl beta-D-glucoside | mutant T508I/E649C/N667A, pH 6.6, 37°C | Cellulomonas uda | |
5.3 | - |
D-glucose | mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C | Cellulomonas uda | |
6 | - |
methyl alpha-D-glucoside | mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C | Cellulomonas uda | |
6.3 | - |
D-glucose | mutant T508I/E649C/N667A, pH 6.6, 37°C | Cellulomonas uda | |
6.8 | - |
methyl beta-D-glucoside | mutant T508I/E649C/N667A, pH 6.6, 37°C | Cellulomonas uda | |
7.9 | - |
methyl beta-D-glucoside | mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C | Cellulomonas uda | |
8.7 | - |
ethyl beta-D-glucoside | mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C | Cellulomonas uda | |
9 | - |
butyl beta-D-glucoside | mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C | Cellulomonas uda |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
methyl alpha-D-glucoside | mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C | Cellulomonas uda | |
0.031 | - |
D-glucose | mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C | Cellulomonas uda | |
0.033 | - |
ethyl beta-D-glucoside | mutant T508I/E649C/N667A, pH 6.6, 37°C | Cellulomonas uda | |
0.063 | - |
ethyl beta-D-glucoside | mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C | Cellulomonas uda | |
0.099 | - |
methyl beta-D-glucoside | mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C | Cellulomonas uda | |
0.12 | - |
methyl beta-D-glucoside | mutant T508I/E649C/N667A, pH 6.6, 37°C | Cellulomonas uda | |
0.129 | - |
phenyl beta-D-glucoside | mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C | Cellulomonas uda | |
0.217 | - |
D-glucose | mutant T508I/E649C/N667A, pH 6.6, 37°C | Cellulomonas uda | |
0.24 | - |
cellobiose | mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C | Cellulomonas uda | |
0.266 | - |
butyl beta-D-glucoside | mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C | Cellulomonas uda |