EC Number   |
Protein Variants |
Reference |
|---|
    2.3.1.51 | 140delF |
34.8% of wild-type activity |
657783 |
| 2.3.1.51 | 221delGT |
5% of wild-type activity |
657783 |
| 2.3.1.51 | 252delMRT |
3.2% of wild-type activity |
657783 |
| 2.3.1.51 | A239V |
90% of wild-type activity |
657783 |
| 2.3.1.51 | C247Y |
loss of the ability of the plastidial enzyme to restore growth to JC201, suggests that C247 is essential for activity |
487209 |
| 2.3.1.51 | D168R |
site-directed mutagenesis, the mutant shows slightly increased activity compared to the wild-type AGPAT8 |
671597 |
| 2.3.1.51 | D180fsX251 |
7.7% of wild-type activity |
657783 |
| 2.3.1.51 | D199A |
the mutant fails to grow beyond 0.04 D600 after transfer to the non-permissive temperature. D199 is essential for the ability of the plastidial enzyme to complement the defective acyltransferase activity of JC201 |
487209 |
| 2.3.1.51 | E260K |
site-directed mutagenesis, mutation found in Chanarin-Dorfman syndrome patients, mutation without significant effect on acyltransferase activity |
687752 |
| 2.3.1.51 | E266A |
the plastidial enzyme containing this mutation is unable to complement the temperature-sensitive phenotype of JC201, indicating an essential role for this acidic residue |
487209 |
