Cloned (Comment) | Organism |
---|---|
transformation of a modified plasmid into Escherichia coli JC201 enzyme-deficient strain and transformation of the plasmid DNA containing the cDNA BAT2 encoding the plastidial enzyme into the Escherichia coli XL-1 red mutator strain | Brassica napus |
Protein Variants | Comment | Organism |
---|---|---|
C247Y | loss of the ability of the plastidial enzyme to restore growth to JC201, suggests that C247 is essential for activity | Brassica napus |
D199A | the mutant fails to grow beyond 0.04 D600 after transfer to the non-permissive temperature. D199 is essential for the ability of the plastidial enzyme to complement the defective acyltransferase activity of JC201 | Brassica napus |
E266A | the plastidial enzyme containing this mutation is unable to complement the temperature-sensitive phenotype of JC201, indicating an essential role for this acidic residue | Brassica napus |
G140S | loss of the ability of the plastidial enzyme to restore growth to JC201, suggests that G140 is essential for activity | Brassica napus |
H194A | the mutant fails to grow beyond 0.04 D600 after transfer to the non-permissive temperature. H194 is essential for the ability of the plastidial enzyme to complement the defective acyltransferase activity of JC201 | Brassica napus |
K84Q | greatly impaired growth rate, possibly indicating an importance of the N-terminus of the mature protein in determining enzyme activity | Brassica napus |
L203P | loss of the ability of the plastidial enzyme to restore growth to JC201, suggests that L203 is essential for activity | Brassica napus |
P187L | loss of the ability of the plastidial enzyme to restore growth to JC201, suggests that P187 is essential for activity | Brassica napus |
R92G | greatly impaired growth rate, possibly indicating an importance of the N-terminus of the mature protein in determining enzyme activity | Brassica napus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plastid | - |
Brassica napus | 9536 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica napus | Q9XFW4 | - |
- |