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Literature summary for 2.3.1.51 extracted from
- Functional characterization of human 1-acylglycerol-3-phosphate acyltransferase isoform 8: cloning, tissue distribution, gene structure, and enzymatic activity (2006), Arch. Biochem. Biophys., 449, 64-76.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene AGPAT8, DNA and amino acid sequence determination and analysis, localization on chromosome 17, genetic structure, expression analysis | Mus musculus |
| gene AGPAT8, DNA and amino acid sequence determination and analysis, localization on chromosome 2p23.1, genetic structure, expression analysis, stable expression of wild-type and mutant enzymes in CHO cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D168R | site-directed mutagenesis, the mutant shows slightly increased activity compared to the wild-type AGPAT8 | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | Mus musculus | - |
CoA + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? |  |
| acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | Homo sapiens | - |
CoA + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
| additional information | Homo sapiens | AGPAT8 shows moderate acyltransferase activity with oleoyl-CoA but lacks acyl-CoA:lysocardiolipin acyltransferase activity, human AGPAT2, acylation at the sn-2 position with wild-type AGPAT8 is similar for the LPA species containing oleoyl, linoleoyl, linolenoyl, and palmitoyl fatty acids at the sn-1 position, LPA species containing myristoyl, arachidoyl, arachidonoyl are not preferred, substrate specificity in vivo, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
gene AGPAT8 | - |
| Mus musculus | - |
gene AGPAT8 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adipocyte | - |
Homo sapiens | - |
| brain | - |
Homo sapiens | - |
| colon | - |
Homo sapiens | - |
| heart | - |
Homo sapiens | - |
| kidney | - |
Homo sapiens | - |
| leukocyte | - |
Homo sapiens | - |
| liver | - |
Mus musculus | - |
| liver | - |
Homo sapiens | - |
| lung | - |
Homo sapiens | - |
| additional information | tissue-specific expression analysis of AGPAT8 | Mus musculus | - |
| additional information | tissue-specific expression analysis of AGPAT8 | Homo sapiens | - |
| ovary | - |
Homo sapiens | - |
| pancreas | - |
Homo sapiens | - |
| placenta | - |
Homo sapiens | - |
| prostate | - |
Homo sapiens | - |
| skeletal muscle | - |
Mus musculus | - |
| skeletal muscle | - |
Homo sapiens | - |
| small intestine | - |
Homo sapiens | - |
| spleen | - |
Homo sapiens | - |
| testis | - |
Homo sapiens | - |
| thymus | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
AGPAT8 has moderate acyltransferase activity, only 18.4% when compared to the other isoform, AGPAT2, using oleoyl-CoA as the acyl donor and 1-oleoyl-3-phosphate-glycerol as the acceptor | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | - |
Mus musculus | CoA + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
| acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | - |
Homo sapiens | CoA + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
| acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | the enzyme catalyzes the transfer of the fatty acid from an acyl donor to the sn-2-position of lysophosphatidic acid | Mus musculus | CoA + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
| acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | the enzyme catalyzes the transfer of the fatty acid from an acyl donor to the sn-2-position of lysophosphatidic acid | Homo sapiens | CoA + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
| additional information | AGPAT8 shows moderate acyltransferase activity with oleoyl-CoA but lacks acyl-CoA:lysocardiolipin acyltransferase activity, human AGPAT2, acylation at the sn-2 position with wild-type AGPAT8 is similar for the LPA species containing oleoyl, linoleoyl, linolenoyl, and palmitoyl fatty acids at the sn-1 position, LPA species containing myristoyl, arachidoyl, arachidonoyl are not preferred, substrate specificity in vivo, overview | Homo sapiens | ? | - |
? | |
| additional information | sn-1-acyl-lysophosphatidic acid and acly-CoA specficity, AGPAT8 is unable to use arachidonoyl-CoA as the acyl donor, overview | Homo sapiens | ? | - |
? | |
| oleoyl-CoA + 1-oleoyl-sn-glycerol-3-phosphate | - |
Homo sapiens | CoA + 1,2-dioleoyl-sn-glycerol 3-phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 1-acylglycerol-3-phosphate acyltransferase | - |
Mus musculus |
| 1-acylglycerol-3-phosphate acyltransferase | - |
Homo sapiens |
| AGPAT | - |
Mus musculus |
| AGPAT | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at room temperature | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
assay at | Mus musculus |
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