EC Number |
Protein Variants |
Reference |
---|
3.2.1.62 | D1711N |
introduction of a glycosylation site, reduced transport rate to the plasma membrane |
208744 |
3.2.1.62 | D1711N |
introduction of potential N-glycosylation site, no enzymic activity, probably due to altered protein folding pattern and reduced dimerization efficiency |
208744 |
3.2.1.62 | D311Y |
the mutant enzyme is characterized by a lowered KM value for the hydrolysis of 2-chloro-4-nitrophenyl beta-cellobioside and increased transglycosylation when using aliphatic 1,3-diols or alcohols bearing a delta-hydroxy ketone function as acceptors. The transglycosylation/hydrolysis ratio in reactions catalyzed by the mutant is completely inversed and weak secondary hydrolysis is postponed, thus providing the basis for high transglycosylation yields (between 68 and 93%) |
749451 |
3.2.1.62 | D311Y |
the mutant shows increased catalytic efficiency for the hydrolysis of 2-chloro-4-nitrophenyl beta-cellobioside compared to the wild type enzyme. The transglycosylation ability of the mutant is improved in comparison to the wild type enzyme |
749451 |
3.2.1.62 | E165D |
reduced activity |
680835 |
3.2.1.62 | E165Q |
almost no activity |
680835 |
3.2.1.62 | E233A |
site directed mutagenesis |
680770 |
3.2.1.62 | E254Q |
inactive |
-, 732073 |
3.2.1.62 | E351S |
site directed mutagenesis |
680770 |
3.2.1.62 | E373D |
reduced activity |
680835 |