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Literature summary for 3.2.1.62 extracted from

  • Caines, M.E.; Vaughan, M.D.; Tarling, C.A.; Hancock, S.M.; Warren, R.A.; Withers, S.G.; Strynadka, N.C.
    Structural and mechanistic analyses of endo-glycoceramidase II, a membrane-associated family 5 glycosidase in the Apo and GM3 ganglioside-bound forms (2007), J. Biol. Chem., 282, 14300-14308.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of the gene lacking the 30-residue N-terminal secretion signal sequence in Escherichia coli, selenomethionine-derivatized enzyme Rhodococcus sp.

Crystallization (Commentary)

Crystallization (Comment) Organism
by vapor diffusion in the presence of detergents like Triton X-100, octyl beta-D-glucopyranoside, decyl beta-D-maltopyranoside, and dodecyl beta-D-maltopyranoside, form, in complex with the ganglioside GM3 Rhodococcus sp.

Protein Variants

Protein Variants Comment Organism
E233A site directed mutagenesis Rhodococcus sp.
E351S site directed mutagenesis Rhodococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
D-erythro-sphingosine
-
Rhodococcus sp.
0.3
-
D-erythro-dihydrosphingosine
-
Rhodococcus sp.

Organism

Organism UniProt Comment Textmining
Rhodococcus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
of the recombinant protein by by Ni(II) affinity chromatography Rhodococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-erythro-dihydrosphingosine + a sugar
-
Rhodococcus sp. ?
-
?
D-erythro-sphingosine + a sugar
-
Rhodococcus sp. ?
-
?

Synonyms

Synonyms Comment Organism
EGC
-
Rhodococcus sp.
endo-glycoceramidase II membrane-associated family 5 glycosidase Rhodococcus sp.
glycosylceramidase
-
Rhodococcus sp.