EC Number |
Protein Variants |
Reference |
---|
2.7.4.14 | more |
two types of chimeric enzymes have been constructed by genetic engineering of chicken cytosolic adenylate kinase and porcine brain UMP/CMP kinase. One designated as UAU carries an AMP-binding domain of AK in the remaining body of UMP/CMP kinase, and the other, designated as AUA, carries a UMP/CMP-binding domain in the remaining body of adenylate kinase. UAU is 4fold more active with AMP, 40fold less active with UMP, and 4fold less active with CMP than the parental UMP/CMP kinase, although AUA has considerably lowered reactivity for both AMP and UMP. AUA has a Tm-value 11°C lower than adenylate kinase, whereas UAU has a Tm-value similar to that of UMP/CMP kinase. Expression in Escherichia coli JM109 |
645157 |
2.7.4.14 | G21A |
mutant enzyme is degraded during the purification phase |
645158 |
2.7.4.14 | G22A |
mutant enzyme with decreased turnover-number/KmATP value. Turnover-number is 59% of that of the wild-type enzyme |
645158 |
2.7.4.14 | G24A |
mutant enzyme with decreased turnover-number/KmATP value. Turnover-number is 48% of that of the wild-type enzyme |
645158 |
2.7.4.14 | G26A |
mutant enzyme with decreased turnover-number/KmATP value |
645158 |
2.7.4.14 | G27R |
mutant enzyme is degraded during the purification phase. Turnover-number is 45% of that of the wild-type enzyme |
645158 |
2.7.4.14 | K27E |
mutant enzyme with 2600fold decreased turnover-number/KmATP value. Turnover-number is 21% of that of the wild-type enzyme |
645158 |
2.7.4.14 | K27M |
mutant enzyme with 1000fold decreased turnover-number/KmATP value. Turnover-number is 22% of that of the wild-type enzyme |
645158 |
2.7.4.14 | more |
determination and analysis of 28 polymorphisms in DCK/CMPK enzymes, genotyping with humans of different ethnic origins, overview. Variant allozyme enzyme activities range from 32 to 105% of the wild-type enzyme for DCK and from 78 to 112% of wild-type enzyme for CMPK, with no significant differences in apparent Km values for either enzyme except for a DCK Val24/Ser122 double variant allozyme, overview |
692040 |
2.7.4.14 | E448G |
site-directed mutagenesis |
693063 |