EC Number |
Protein Variants |
Reference |
---|
2.5.1.27 | D49A |
almost complete loss of activity compared to the wild type enzyme |
671796 |
2.5.1.27 | F120A |
almost complete loss of activity compared to the wild type enzyme |
671796 |
2.5.1.27 | F157A |
almost complete loss of activity compared to the wild type enzyme |
671796 |
2.5.1.27 | F93A |
almost complete loss of activity compared to the wild type enzyme |
671796 |
2.5.1.27 | Q255A |
almost complete loss of activity compared to the wild type enzyme |
671796 |
2.5.1.27 | W159A |
almost complete loss of activity compared to the wild type enzyme |
671796 |
2.5.1.27 | Y153A |
almost complete loss of activity compared to the wild type enzyme |
671796 |
2.5.1.27 | Y217A |
almost complete loss of activity compared to the wild type enzyme |
671796 |
2.5.1.27 | Y54A |
almost complete loss of activity compared to the wild type enzyme |
671796 |
2.5.1.27 | H214L |
decrease in activity with substrate 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate. Specificity ratio for substrate dimethylallyl diphosphate over 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate is 1163 compared with 2.3 for wild-type |
689799 |