BRENDA - Enzyme Database show
show all sequences of 2.5.1.27

Structural insight into the reaction mechanism and evolution of cytokinin biosynthesis

Sugawara, H.; Ueda, N.; Kojima, M.; Makita, N.; Yamaya, T.; Sakakibara, H.; Proc. Natl. Acad. Sci. USA 105, 2734-2739 (2008)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
isoform Tzs in complex with AMP and dimethylallyl S-thiodiphosphate. The carbon-nitrogen-based prenylation proceeds by the SN2-reaction mechanism
Agrobacterium tumefaciens
Engineering
Amino acid exchange
Commentary
Organism
D173G
decrease in activity with substrate 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate. Specificity ratio for substrate dimethylallyl diphosphate over 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate is 208 compared with 2.3 for wild-type
Agrobacterium tumefaciens
E210N
slight decrease in activity with substrate 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate. Specificity ratio for substrate dimethylallyl diphosphate over 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate is 15 compared with 2.3 for wild-type
Agrobacterium tumefaciens
E213Q
slight decrease in activity with substrate 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate. Specificity ratio for substrate dimethylallyl diphosphate over 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate is 5.1 compared with 2.3 for wild-type
Agrobacterium tumefaciens
H214L
decrease in activity with substrate 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate. Specificity ratio for substrate dimethylallyl diphosphate over 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate is 1163 compared with 2.3 for wild-type
Agrobacterium tumefaciens
Y211T
slight decrease in activity with substrate 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate. Specificity ratio for substrate dimethylallyl diphosphate over 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate is 9 compared with 2.3 for wild-type
Agrobacterium tumefaciens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00023
-
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate
mutant Y211T
Agrobacterium tumefaciens
0.0026
-
dimethylallyl diphosphate
mutant D173G
Agrobacterium tumefaciens
0.0042
-
dimethylallyl diphosphate
mutant E210N
Agrobacterium tumefaciens
0.0075
-
dimethylallyl diphosphate
mutant E213Q
Agrobacterium tumefaciens
0.0079
-
dimethylallyl diphosphate
wild-type
Agrobacterium tumefaciens
0.0082
-
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate
wild-type
Agrobacterium tumefaciens
0.0108
-
dimethylallyl diphosphate
mutant H214L
Agrobacterium tumefaciens
0.0226
-
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate
mutant D173G
Agrobacterium tumefaciens
0.0261
-
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate
mutant E210N
Agrobacterium tumefaciens
0.0335
-
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate
mutant E213Q
Agrobacterium tumefaciens
0.035
-
AMP
wild-type
Agrobacterium tumefaciens
0.0354
-
dimethylallyl diphosphate
mutant Y211T
Agrobacterium tumefaciens
0.0805
-
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate
mutant H214L
Agrobacterium tumefaciens
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Zn2+
two Zn2+ ions are present in the crystal. One is coordinated with the hydroxyl group of Thr-15. The other is located near AMP and is coordinated to the side chains of Asp-173, Glu-213, and His-214
Agrobacterium tumefaciens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Agrobacterium tumefaciens
P58758
isoform Tzs
-
Reaction
Reaction
Commentary
Organism
dimethylallyl diphosphate + AMP = diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
the carbon-nitrogen-based prenylation proceeds by the SN2-reaction mechanism
Agrobacterium tumefaciens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate + AMP
-
689799
Agrobacterium tumefaciens
diphosphate + N6-(4-hydroxy-3-methyl-2-(E)-butyl)adenosine 5'-phosphate
-
-
-
?
dimethylallyl diphosphate + AMP
-
689799
Agrobacterium tumefaciens
diphosphate + N-(dimethylallyl)adenosine 5'-phosphate
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.00023
-
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate
mutant Y211T
Agrobacterium tumefaciens
0.00058
-
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate
mutant H214L
Agrobacterium tumefaciens
0.001
-
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate
mutant D173G
Agrobacterium tumefaciens
0.002
-
dimethylallyl diphosphate
mutant Y211T
Agrobacterium tumefaciens
0.01
-
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate
mutant E210N
Agrobacterium tumefaciens
0.023
-
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate
mutant E213Q
Agrobacterium tumefaciens
0.023
-
dimethylallyl diphosphate
mutant D173G; mutant E210N
Agrobacterium tumefaciens
0.025
-
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate
wild-type
Agrobacterium tumefaciens
0.033
-
dimethylallyl diphosphate
mutant E213Q
Agrobacterium tumefaciens
0.053
-
dimethylallyl diphosphate
wild-type
Agrobacterium tumefaciens
0.09
-
dimethylallyl diphosphate
mutant H214L
Agrobacterium tumefaciens
Crystallization (Commentary) (protein specific)
Crystallization
Organism
isoform Tzs in complex with AMP and dimethylallyl S-thiodiphosphate. The carbon-nitrogen-based prenylation proceeds by the SN2-reaction mechanism
Agrobacterium tumefaciens
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
D173G
decrease in activity with substrate 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate. Specificity ratio for substrate dimethylallyl diphosphate over 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate is 208 compared with 2.3 for wild-type
Agrobacterium tumefaciens
E210N
slight decrease in activity with substrate 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate. Specificity ratio for substrate dimethylallyl diphosphate over 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate is 15 compared with 2.3 for wild-type
Agrobacterium tumefaciens
E213Q
slight decrease in activity with substrate 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate. Specificity ratio for substrate dimethylallyl diphosphate over 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate is 5.1 compared with 2.3 for wild-type
Agrobacterium tumefaciens
H214L
decrease in activity with substrate 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate. Specificity ratio for substrate dimethylallyl diphosphate over 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate is 1163 compared with 2.3 for wild-type
Agrobacterium tumefaciens
Y211T
slight decrease in activity with substrate 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate. Specificity ratio for substrate dimethylallyl diphosphate over 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate is 9 compared with 2.3 for wild-type
Agrobacterium tumefaciens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00023
-
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate
mutant Y211T
Agrobacterium tumefaciens
0.0026
-
dimethylallyl diphosphate
mutant D173G
Agrobacterium tumefaciens
0.0042
-
dimethylallyl diphosphate
mutant E210N
Agrobacterium tumefaciens
0.0075
-
dimethylallyl diphosphate
mutant E213Q
Agrobacterium tumefaciens
0.0079
-
dimethylallyl diphosphate
wild-type
Agrobacterium tumefaciens
0.0082
-
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate
wild-type
Agrobacterium tumefaciens
0.0108
-
dimethylallyl diphosphate
mutant H214L
Agrobacterium tumefaciens
0.0226
-
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate
mutant D173G
Agrobacterium tumefaciens
0.0261
-
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate
mutant E210N
Agrobacterium tumefaciens
0.0335
-
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate
mutant E213Q
Agrobacterium tumefaciens
0.035
-
AMP
wild-type
Agrobacterium tumefaciens
0.0354
-
dimethylallyl diphosphate
mutant Y211T
Agrobacterium tumefaciens
0.0805
-
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate
mutant H214L
Agrobacterium tumefaciens
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Zn2+
two Zn2+ ions are present in the crystal. One is coordinated with the hydroxyl group of Thr-15. The other is located near AMP and is coordinated to the side chains of Asp-173, Glu-213, and His-214
Agrobacterium tumefaciens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate + AMP
-
689799
Agrobacterium tumefaciens
diphosphate + N6-(4-hydroxy-3-methyl-2-(E)-butyl)adenosine 5'-phosphate
-
-
-
?
dimethylallyl diphosphate + AMP
-
689799
Agrobacterium tumefaciens
diphosphate + N-(dimethylallyl)adenosine 5'-phosphate
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.00023
-
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate
mutant Y211T
Agrobacterium tumefaciens
0.00058
-
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate
mutant H214L
Agrobacterium tumefaciens
0.001
-
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate
mutant D173G
Agrobacterium tumefaciens
0.002
-
dimethylallyl diphosphate
mutant Y211T
Agrobacterium tumefaciens
0.01
-
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate
mutant E210N
Agrobacterium tumefaciens
0.023
-
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate
mutant E213Q
Agrobacterium tumefaciens
0.023
-
dimethylallyl diphosphate
mutant D173G; mutant E210N
Agrobacterium tumefaciens
0.025
-
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate
wild-type
Agrobacterium tumefaciens
0.033
-
dimethylallyl diphosphate
mutant E213Q
Agrobacterium tumefaciens
0.053
-
dimethylallyl diphosphate
wild-type
Agrobacterium tumefaciens
0.09
-
dimethylallyl diphosphate
mutant H214L
Agrobacterium tumefaciens
Other publictions for EC 2.5.1.27
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739429
Frebortova
Biochemical characterization o ...
Nostoc sp.
PLoS ONE
10
e0138468
2015
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1
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5
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1
2
2
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7
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1
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1
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6
1
1
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2
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1
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6
2
1
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2
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2
2
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721952
Yeh
-
Molecular characterization of ...
Bambusa oldhamii
Bot. Stud.
52
249-256
2011
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1
1
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723389
Qin
Regulated expression of an iso ...
Arachis hypogaea
Plant Cell Physiol.
52
1904-1914
2011
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1
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1
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6
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1
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705984
Chu
Crystal structure and substrat ...
Humulus lupulus
Nucleic Acids Res.
38
1738-1748
2010
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1
1
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2
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5
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2
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1
1
-
-
-
721466
Wagiran
-
Agrobacterium tumefaciens-medi ...
Oryza sativa
Aust. J. Crop Sci.
4
421-429
2010
-
-
1
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1
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1
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1
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1
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722266
Chu
Binding and catalysis of Humul ...
Humulus lupulus
FEBS Lett.
584
4083-4088
2010
-
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1
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3
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7
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1
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1
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1
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7
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1
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1
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2
2
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722898
Zhang
Senescence-inducible expressio ...
Manihot esculenta
J. Integr. Plant Biol.
52
653-669
2010
-
-
1
-
1
-
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-
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1
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3
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2
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1
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1
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1
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1
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2
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1
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-
1
2
2
1
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-
688633
Ma
-
Expression of isopentenyl tran ...
Agrobacterium tumefaciens
J. Plant Growth Regul.
27
68-76
2008
-
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1
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1
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1
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1
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1
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689564
Brugiere
A member of the maize isopente ...
Zea mays
Plant Mol. Biol.
67
215-229
2008
-
-
1
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1
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3
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6
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4
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1
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1
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6
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4
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-
-
-
-
-
-
689799
Sugawara
Structural insight into the re ...
Agrobacterium tumefaciens
Proc. Natl. Acad. Sci. USA
105
2734-2739
2008
-
-
-
1
5
-
-
13
-
1
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-
5
-
-
-
1
-
-
-
-
2
-
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11
-
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1
5
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-
13
-
1
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-
2
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11
-
-
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-
706291
Galichet
Farnesylation directs AtIPT3 s ...
Arabidopsis thaliana
Plant Physiol.
146
1155-1164
2008
-
-
1
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-
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3
-
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3
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1
1
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1
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1
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3
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1
1
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
671796
Abe
Enzymatic formation of unnatur ...
Morus alba
Biochem. Biophys. Res. Commun.
355
795-800
2007
-
-
1
-
10
-
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2
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2
1
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3
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1
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1
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12
-
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2
1
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3
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-
1
3
-
10
-
-
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2
-
2
1
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1
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1
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-
12
-
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2
1
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-
-
676481
Ballester
Efficient production of transg ...
Citrus sinensis, Citrus sinensis x Citrus trifoliata
Plant Cell Rep.
26
39-45
2007
-
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-
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7
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2
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