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Information on EC 2.5.1.27 - adenylate dimethylallyltransferase
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EC Tree
2.5.1.27 adenylate dimethylallyltransferaseIUBMB Comments
Involved in the biosynthesis of cytokinins in plants. Some isoforms from the plant Arabidopsis thaliana are specific for AMP while others also have the activity of EC 2.5.1.112, adenylate dimethylallyltransferase (ADP/ATP-dependent).
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Word Map
- 2.5.1.27
- tumefaciens
- trans-zeatin
-
marker-free
- zeatin
- isopentenyladenine
-
tz-type
-
bentgrass
- stolonifera
-
senescence-activated
- cis-zeatin
-
agrostis
- synthesis
The enzyme appears in viruses and cellular organisms
Reaction Schemes
Synonyms
isopentenyl transferase, isopentenyltransferase, sag12-ipt, atipt3, atipt5, adenosine phosphate-isopentenyltransferase, ipt5b, atipt7, adenylate isopentenyltransferase, atipt4, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
2-isopentenyl-diphosphate:AMP DELTA2-isopentenyltransferase
-
-
-
-
adenylate dimethylallyltransferase
adenylate IPT
adenylate isopentenyl transferase
adenylate isopentenyltransferase
adenylate-type isopentenyl transferase
AIPT
AtIPT1
AtIPT4
cytokinin synthase
-
-
-
-
dimethylallyl-diphosphate:AMP DELTA2-isopentenyltransferase
-
-
-
-
dimethylallylpyrophosphate:5'-AMP transferase
-
-
-
-
DMA transferase
-
-
-
-
DMAPP:AMP isopentenyltransferase
HlAIPT
adenylate isopentenyltransferase-ATP complex from Humulus lupulus
IPT
IPT1
isopentenyl transferase
isopentenyltransferase
isopentenyltransferase, adenylate
-
-
-
-
NoIPT1
isopentenyltransferase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
dimethylallyl diphosphate + AMP = diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
-
-
-
-
dimethylallyl diphosphate + AMP = diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
the carbon-nitrogen-based prenylation proceeds by the SN2-reaction mechanism
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
alkenyl group transfer
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
dimethylallyl-diphosphate:AMP dimethylallyltransferase
Involved in the biosynthesis of cytokinins in plants. Some isoforms from the plant Arabidopsis thaliana are specific for AMP while others also have the activity of EC 2.5.1.112, adenylate dimethylallyltransferase (ADP/ATP-dependent).
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CAS REGISTRY NUMBER
COMMENTARY
72840-95-0
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(E)-4-hydroxy-3-methyl-but-2-enyl diphosphate + AMP
diphosphate + trans-zeatin riboside 5'phosphate
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate + AMP
diphosphate + N6-(4-hydroxy-3-methyl-2-(E)-butyl)adenosine 5'-phosphate
4-hydroxy-3-methyl-2-(E)-butenyl diphosphate + ADP
diphosphate + N6-(4-hydroxy-3-methyl-2-(E)-butenyl)ADP
4-hydroxy-3-methyl-2-(E)-butenyl diphosphate is a very poor substrate
-
-
?
4-hydroxy-3-methyl-2-(E)-butenyl diphosphate + AMP
diphosphate + N6-(4-hydroxy-3-methyl-2-(E)-butyl)adenosine 5'-phosphate
-
-
-
-
?
4-hydroxy-3-methyl-2-(E)-butenyl diphosphate + ATP
trans-zeatin riboside 5'-triphosphate + diphosphate
4-hydroxy-3-methyl-2-(E)-butenyl diphosphate is a very poor substrate
-
-
?
dimethylallyl diphosphate + ADP
dimethylallyl-ADP + diphosphate
the enzyme preferrs ADP and ATP rather than AMP
-
-
?
dimethylallyl diphosphate + ADP
diphosphate + N-(dimethylallyl)adenosine 5'-diphosphate
ADP and ATP are preferred over AMP
-
-
?
dimethylallyl diphosphate + ATP
dimethylallyl-ATP + diphosphate
the enzyme preferrs ADP and ATP rather than AMP
-
-
?
dimethylallyl diphosphate + ATP
diphosphate + N-(dimethylallyl)adenosine 5'-triphosphate
ADP and ATP are preferred over AMP
-
-
?
dimethylallyl diphosphate + ATP
diphosphate + N6-(dimethylallyl)adenosine triphosphate
-
-
-
-
?
dimethylallyl diphosphate + ATP
N6-(dimethylallyl) adenosine 5'-triphosphate + diphosphate
-
-
-
ir
dimethylallyl diphosphate + dADP
diphosphate + N6-(dimethylallyl)dADP
-
-
-
ir
dimethylallyl diphosphate + dATP
diphosphate + N6-(dimethylallyl)dATP
-
-
-
ir
geranyl diphosphate + ADP
diphosphate + N6-geranyl-ADP
geranyl diphosphate is a very poor substrate
-
-
?
isopentenyl diphosphate + ADP
diphosphate + N6-isopentyl-ADP
isopentyl diphosphate is a very poor substrate
-
-
?
(E)-4-hydroxy-3-methyl-but-2-enyl diphosphate + AMP
diphosphate + trans-zeatin riboside 5'phosphate
HMBPP, the enzyme uses the hydroxylated side chain precursor to form trans-zeatin-5'-monophosphate in the reaction with AMP, reaction rate is 0.5% of the rate with dimethylallyl díphosphate
-
-
?
(E)-4-hydroxy-3-methyl-but-2-enyl diphosphate + AMP
diphosphate + trans-zeatin riboside 5'phosphate
HMBPP, the enzyme uses the hydroxylated side chain precursor to form trans-zeatin-5'-monophosphate in the reaction with AMP, reaction rate is 0.5% of the rate with dimethylallyl díphosphate
-
-
?
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate + AMP
diphosphate + N6-(4-hydroxy-3-methyl-2-(E)-butyl)adenosine 5'-phosphate
-
-
-
-
?
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate + AMP
diphosphate + N6-(4-hydroxy-3-methyl-2-(E)-butyl)adenosine 5'-phosphate
-
-
-
?
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate + AMP
diphosphate + N6-(4-hydroxy-3-methyl-2-(E)-butyl)adenosine 5'-phosphate
-
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate is an intermediate in the methylerythritol phosphate pathway. Agrobacterium tumefaciens modifies cytokinin biosynthesis by sending a key enzyme into plastids of the host plant to promote tumorigenesis
-
-
?
dimethylallyl diphosphate + ADP
?
-
the enzyme prefers ADP and ATP rather than AMP
-
-
?
dimethylallyl diphosphate + ADP
?
the enzyme prefers ADP and ATP rather than AMP
-
-
?
dimethylallyl diphosphate + ADP
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
-
-
-
ir
dimethylallyl diphosphate + ADP
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
-
-
-
?
dimethylallyl diphosphate + AMP
diphosphate + N-(dimethylallyl)adenosine 5'-phosphate
-
-
-
?
dimethylallyl diphosphate + AMP
diphosphate + N-(dimethylallyl)adenosine 5'-phosphate
-
-
-
ir
dimethylallyl diphosphate + AMP
diphosphate + N-(dimethylallyl)adenosine 5'-phosphate
ADP and ATP are preferred over AMP
-
-
?
dimethylallyl diphosphate + AMP
diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
-
-
-
-
?
dimethylallyl diphosphate + AMP
diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
-
ATP, ADP and cAMP do not function as isopentenyl acceptors
-
-
?
dimethylallyl diphosphate + AMP
diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
-
-
-
-
?
dimethylallyl diphosphate + AMP
diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
-
-
-
?
dimethylallyl diphosphate + AMP
diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
biosynthesis of cytokinins
-
-
?
dimethylallyl diphosphate + AMP
diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
the enzyme preferrs ADP and ATP rather than AMP
-
-
?
dimethylallyl diphosphate + AMP
diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
-
the enzyme prefers ADP and ATP rather than AMP
-
-
?
dimethylallyl diphosphate + AMP
diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
-
-
-
?
dimethylallyl diphosphate + AMP
diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
-
ATP, ADP and cAMP do not function as isopentenyl acceptors
-
-
?
dimethylallyl diphosphate + AMP
diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
-
-
-
-
?
dimethylallyl diphosphate + AMP
diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
the enzyme prefers ADP and ATP rather than AMP
-
-
?
dimethylallyl diphosphate + AMP
diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
-
-
-
-
?
dimethylallyl diphosphate + AMP
diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
AMP is a poor substrate
-
-
ir
dimethylallyl diphosphate + AMP
diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
-
-
-
?
dimethylallyl diphosphate + AMP
diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
-
-
-
-
?
dimethylallyl diphosphate + AMP
diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
-
biosynthesis of cytokinins
-
-
?
dimethylallyl diphosphate + AMP
diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
-
-
-
?
dimethylallyl diphosphate + AMP
diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
-
-
-
?
dimethylallyl diphosphate + AMP
diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
-
-
-
?
dimethylallyl diphosphate + ATP
?
-
the enzyme prefers ADP and ATP rather than AMP
-
-
?
dimethylallyl diphosphate + ATP
?
the enzyme prefers ADP and ATP rather than AMP
-
-
?
dimethylallyl diphosphate + ATP
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
-
-
-
ir
dimethylallyl diphosphate + ATP
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
-
-
-
?
additional information
?
-
no substrate: adenine, adenosine, isopentenyldiphosphate
-
-
?
additional information
?
-
-
no substrate: adenine, adenosine, isopentenyldiphosphate
-
-
?
additional information
?
-
-
AtIPT3 is upregulated within 1 h after an application of nitrate zo mineral-strved Arabidopsis plants
-
-
?
additional information
?
-
-
no activity with 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate
-
-
?
additional information
?
-
no activity with GMP, IMP, CMP and UMP
-
-
?
additional information
?
-
-
the plant AIPT predominantly uses ATP or ADP as the isopentenyl acceptor
-
-
?
additional information
?
-
farnesyl diphosphate, geranylgeranyl diphosphate, IDP and UDP are no substrates for Morus alba AIPT
-
-
?
additional information
?
-
-
farnesyl diphosphate, geranylgeranyl diphosphate, IDP and UDP are no substrates for Morus alba AIPT
-
-
?
additional information
?
-
-
strict requirement for AMP, no substrate: adenine, adenosine
-
-
?
additional information
?
-
-
less than 0.5% activity with (E)-4-hydroxy-3-methyl-but-2-enyl diphosphate plus AMP
-
-
?
additional information
?
-
less than 0.5% activity with (E)-4-hydroxy-3-methyl-but-2-enyl diphosphate plus AMP
-
-
?
additional information
?
-
-
no activity with ADP and ATP. Isopentenyl diphosphate, an isomer of DMAPP, is no substrate with either of the side chain acceptor substrates. The enzyme does not show tRNA IPT activity, as it does not prenylate synthetic tRNA-stem-loop minihelix substrate
-
-
?
additional information
?
-
no activity with ADP and ATP. Isopentenyl diphosphate, an isomer of DMAPP, is no substrate with either of the side chain acceptor substrates. The enzyme does not show tRNA IPT activity, as it does not prenylate synthetic tRNA-stem-loop minihelix substrate
-
-
?
additional information
?
-
-
the enzyme shows no activity with ATP and ADP and no tRNA isopentenyl transferase activity
-
-
?
additional information
?
-
the enzyme shows no activity with ATP and ADP and no tRNA isopentenyl transferase activity
-
-
?
additional information
?
-
-
less than 0.5% activity with (E)-4-hydroxy-3-methyl-but-2-enyl diphosphate plus AMP
-
-
?
additional information
?
-
less than 0.5% activity with (E)-4-hydroxy-3-methyl-but-2-enyl diphosphate plus AMP
-
-
?
additional information
?
-
-
the enzyme shows no activity with ATP and ADP and no tRNA isopentenyl transferase activity
-
-
?
additional information
?
-
the enzyme shows no activity with ATP and ADP and no tRNA isopentenyl transferase activity
-
-
?
additional information
?
-
-
no activity with ADP and ATP. Isopentenyl diphosphate, an isomer of DMAPP, is no substrate with either of the side chain acceptor substrates. The enzyme does not show tRNA IPT activity, as it does not prenylate synthetic tRNA-stem-loop minihelix substrate
-
-
?
additional information
?
-
no activity with ADP and ATP. Isopentenyl diphosphate, an isomer of DMAPP, is no substrate with either of the side chain acceptor substrates. The enzyme does not show tRNA IPT activity, as it does not prenylate synthetic tRNA-stem-loop minihelix substrate
-
-
?
additional information
?
-
no substrate: 1-hydroxy-2-methyl-2-buten-4-yl 4-diphosphate
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate + AMP
diphosphate + N6-(4-hydroxy-3-methyl-2-(E)-butyl)adenosine 5'-phosphate
-
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate is an intermediate in the methylerythritol phosphate pathway. Agrobacterium tumefaciens modifies cytokinin biosynthesis by sending a key enzyme into plastids of the host plant to promote tumorigenesis
-
-
?
dimethylallyl diphosphate + AMP
diphosphate + N-(dimethylallyl)adenosine 5'-phosphate
-
-
-
ir
dimethylallyl diphosphate + ATP
N6-(dimethylallyl) adenosine 5'-triphosphate + diphosphate
-
-
-
ir
dimethylallyl diphosphate + AMP
diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
-
-
-
-
?
dimethylallyl diphosphate + AMP
diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
biosynthesis of cytokinins
-
-
?
dimethylallyl diphosphate + AMP
diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
-
-
-
?
dimethylallyl diphosphate + AMP
diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
-
-
-
-
?
dimethylallyl diphosphate + AMP
diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
-
biosynthesis of cytokinins
-
-
?
dimethylallyl diphosphate + AMP
diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
-
-
-
?
dimethylallyl diphosphate + AMP
diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
-
-
-
?
additional information
?
-
-
AtIPT3 is upregulated within 1 h after an application of nitrate zo mineral-strved Arabidopsis plants
-
-
?
additional information
?
-
-
the plant AIPT predominantly uses ATP or ADP as the isopentenyl acceptor
-
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
MgCl2
dependent on presence of divalent cation, addition of 2 mM MnCl2 is 1.3 times more effective than that of 10 mM MgCl2
MnCl2
dependent on presence of divalent cation, addition of 2 mM MnCl2 is 1.3 times more effective than that of 10 mM MgCl2
Zn2+
two Zn2+ ions are present in the crystal. One is coordinated with the hydroxyl group of Thr-15. The other is located near AMP and is coordinated to the side chains of Asp-173, Glu-213, and His-214
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Dehydration
Photosynthesis, water use, and root viability under water stress as affected by expression of SAG12-ipt controlling cytokinin synthesis in Agrostis stolonifera.
Dehydration
Protein accumulation in leaves and roots associated with improved drought tolerance in creeping bentgrass expressing an ipt gene for cytokinin synthesis.
Hypotension
Systematic review and meta-analysis of propofol versus barbiturates for controlling refractory status epilepticus.
Infections
Agrobacterium tumefaciens tumor morphology root plastid localization and preferential usage of hydroxylated prenyl donor is important for efficient gall formation.
Lung Neoplasms
Ethnic differences in frequencies of gene polymorphisms in the MYCL1 region and modulation of lung cancer patients' survival.
Neoplasms
Agrobacterium tumefaciens tumor morphology root plastid localization and preferential usage of hydroxylated prenyl donor is important for efficient gall formation.
Neoplasms
Deoxyribonuclease I sensitivity of the T-DNA ipt gene is associated with gene expression.
Neoplasms
Tobacco plants carrying a tms locus of Ti-plasmid origin and the Hl-1 allele are tumor prone.
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.00023
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate
mutant Y211T
0.0082
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate
wild-type
0.0226
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate
mutant D173G
0.0261
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate
mutant E210N
0.0335
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate
mutant E213Q
0.0805
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate
mutant H214L
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.00023
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate
mutant Y211T
0.00058
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate
mutant H214L
0.001
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate
mutant D173G
0.01
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate
mutant E210N
0.023
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate
mutant E213Q
0.025
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate
wild-type
0.0235
ADP