EC Number |
Protein Variants |
Reference |
---|
2.5.1.27 | D173G |
decrease in activity with substrate 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate. Specificity ratio for substrate dimethylallyl diphosphate over 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate is 208 compared with 2.3 for wild-type |
689799 |
2.5.1.27 | D49A |
almost complete loss of activity compared to the wild type enzyme |
671796 |
2.5.1.27 | E210N |
slight decrease in activity with substrate 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate. Specificity ratio for substrate dimethylallyl diphosphate over 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate is 15 compared with 2.3 for wild-type |
689799 |
2.5.1.27 | E213Q |
slight decrease in activity with substrate 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate. Specificity ratio for substrate dimethylallyl diphosphate over 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate is 5.1 compared with 2.3 for wild-type |
689799 |
2.5.1.27 | F120A |
almost complete loss of activity compared to the wild type enzyme |
671796 |
2.5.1.27 | F157A |
almost complete loss of activity compared to the wild type enzyme |
671796 |
2.5.1.27 | F93A |
almost complete loss of activity compared to the wild type enzyme |
671796 |
2.5.1.27 | H214L |
decrease in activity with substrate 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate. Specificity ratio for substrate dimethylallyl diphosphate over 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate is 1163 compared with 2.3 for wild-type |
689799 |
2.5.1.27 | more |
ectopic expression of isoform IPT2 in calli and in planta of Arabidopsis thaliana creates phenotypes consistent with cytokinin overproduction |
689564 |
2.5.1.27 | more |
generation of SAG12-IPT transgenic cassava, analysis of the level of leaf retention and altered cytokinin metabolism of transgenic plants via the autoregulatory senescence inhibition system, phenotypes overview |
722898 |