EC Number   |
Protein Variants |
Reference |
|---|
    6.2.1.13 | E218alphaD |
site-directed mutagenesis, comparison of the wild-type CD spectrum with the mutant CD spectrum, structure and reaction kinetics, overview. The mutant shows 1-10% of the wild-type activity, phopshorylation of the mutant is reduced |
693061 |
| 6.2.1.13 | E218alphaQ |
site-directed mutagenesis, comparison of the wild-type CD spectrum with the mutant CD spectrum, structure and reaction kinetics, overview. Inactive mutant, phopshorylation of the mutant is reduced |
693061 |
| 6.2.1.13 | E218Dalpha |
1-10% of wild-type activity |
693061 |
| 6.2.1.13 | G266S |
the Acs mutant does not cause growth arrest in contrast to the wild-type enzyme |
716267 |
| 6.2.1.13 | H252A |
site-directed mutagenesis, alteration of His252 in EhACD effectively eliminate overall enzymatic activity as well as phosphorylation in either direction of the reaction |
744911 |
| 6.2.1.13 | H252A |
the variant has no detectable activity in either direction of the reaction |
744911 |
| 6.2.1.13 | H257alphaD |
site-directed mutagenesis, comparison of the wild-type CD spectrum with the mutant CD spectrum, structure and reaction kinetics, overview. Inactive mutant, which is not phosphorylated at both the alpha- and beta-subunit |
693061 |
| 6.2.1.13 | H257Dalpha |
mutant shows no activity in either direction |
693061 |
| 6.2.1.13 | H533D |
the variant retains about 3% activity compared to wild type enzyme |
744911 |
| 6.2.1.13 | H533E |
the variant retains about 1% activity compared to wild type enzyme |
744911 |
