EC Number   |
Protein Variants |
Reference |
|---|
   2.4.1.90 | I285Y |
site-directed mutagenesis, the mutation converts the betaGALNAcT1 enzyme into an efficient beta4Gal-T1, the N-acetylgalactosaminyltransferase activity is reduced by nearly 1000fold, while the galactosyltransferase activity is enhanced by 80fold |
675421 |
| 2.4.1.90 | more |
the enzyme transfected 7721 hepatocarcinoma cell line is more susceptible to cycloheximide-induced apoptosis than the wild-type cells, cells show increased enzyme activity and expression of Galbeta1,4GlcNAc groups on the cell surface |
659925 |
| 2.4.1.90 | M340H |
the mutant shows about 10% of wild type activity |
758173 |
| 2.4.1.90 | H343A |
the mutant shows about 3% of wild type activity |
758173 |
| 2.4.1.90 | M340E |
the mutant shows about 85% of wild type activity |
758173 |
| 2.4.1.90 | D315A |
the mutant shows about 90% of wild type activity |
758173 |
| 2.4.1.90 | D320A |
when partially activated by Mn2+ binding to the primary site, can be further activated by Co2+ or inhibited by Ca2+, an effect that is the opposite of what is observed with the wild-type enzyme |
489556 |
| 2.4.1.90 | D320E |
when partially activated by Mn2+ binding to the primary site, can be further activated by Co2+ or inhibited by Ca2+, an effect that is the opposite of what is observed with the wild-type enzyme |
489556 |
| 2.4.1.90 | D320N |
when partially activated by Mn2+ binding to the primary site, can be further activated by Co2+ or inhibited by Ca2+, an effect that is the opposite of what is observed with the wild-type enzyme |
489556 |
| 2.4.1.90 | E317A |
when partially activated by Mn2+ binding to the primary site, can be further activated by Co2+ or inhibited by Ca2+, an effect that is the opposite of what is observed with the wild-type enzyme |
489556 |
