Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 0.2 M potassium nitrate, 20% (w/v) PEG 3350 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D315A | the mutant shows about 90% of wild type activity | Homo sapiens |
H343A | the mutant shows about 3% of wild type activity | Homo sapiens |
M340E | the mutant shows about 85% of wild type activity | Homo sapiens |
M340H | the mutant shows about 10% of wild type activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0455 | - |
UDP-alpha-D-galactose | at pH 6.3 and 37°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi apparatus | - |
Homo sapiens | 5794 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | 5 mM used in assay conditions | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72600 | - |
gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-galactose + N-acetyl-D-glucosamine | Homo sapiens | - |
UDP + N-acetyllactosamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Bio-Scale mini profinity nickel cartridge chromatography and Superdex S200 gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-galactose + N-acetyl-D-glucosamine | - |
Homo sapiens | UDP + N-acetyllactosamine | - |
? | |
UDP-alpha-D-galactose + ovalbumin | - |
Homo sapiens | UDP + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 36300, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
B4GALT1 | - |
Homo sapiens |
beta-1,4-galactosyltransferase 1 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.059 | - |
UDP-alpha-D-galactose | at pH 6.3 and 37°C | Homo sapiens |