EC Number   |
Protein Variants |
Reference |
|---|
   2.1.1.179 | G135A |
mutant with drastically increased sensitivity to kanamycin |
702415 |
| 2.1.1.179 | G135A |
no binding of S-adenosyl-L-methionine |
704280 |
| 2.1.1.179 | H50A |
tobramycin MIC is drastically reduced compared to wild-type enzyme |
712741 |
| 2.1.1.179 | H54A |
inactive. Mutation dos not impact 30S binding affinity. Mutant strain is sensitive to kanamycin and gentamicin |
757224 |
| 2.1.1.179 | H54E |
inactive. Mutation dos not impact 30S binding affinity. Mutant strain is sensitive to kanamycin and gentamicin |
757224 |
| 2.1.1.179 | H81A |
tobramycin MIC is identical with that of wild-type RmtB. No change in methylation activity compared to wild-type enzyme |
712741 |
| 2.1.1.179 | K14A |
tobramycin MIC is identical with that of wild-type RmtB. 36% of the methylation activity compared to wild-type enzyme |
712741 |
| 2.1.1.179 | K174A |
tobramycin MIC is drastically reduced compared to wild-type enzyme. 0.7% of the methylation activity compared to wild-type enzyme |
712741 |
| 2.1.1.179 | K199A |
mutant retains S-adenosyl-L-methionine binding |
704280 |
| 2.1.1.179 | K199A |
mutant with drastically increased sensitivity to kanamycin |
702415 |
