EC Number |
Protein Variants |
Reference |
---|
2.1.1.179 | R187S/G212S |
mutant with strongly reduced activity |
702415 |
2.1.1.179 | R236A |
mutant retains S-adenosyl-L-methionine binding |
704280 |
2.1.1.179 | R236A |
mutant with drastically increased sensitivity to kanamycin |
702415 |
2.1.1.179 | R433A |
mutant with drastically increased sensitivity to kanamycin |
702415 |
2.1.1.179 | R48A |
tobramycin MIC is identical with that of wild-type RmtB |
712741 |
2.1.1.179 | R50E |
mutation reduces 30S binding affinity about 11-13fold. Mutant strain is sensitive to kanamycin and gentamicin |
757224 |
2.1.1.179 | R68E |
mutation reduces 30S binding affinity about 11-13fold, resistance to kanamycin and gentamcin is reduced |
757224 |
2.1.1.179 | S83A |
tobramycin MIC is identical with that of wild-type RmtB. 18% of the methylation activity compared to wild-type enzyme |
712741 |
2.1.1.179 | Y56F |
tobramycin MIC is identical with that of wild-type RmtB |
712741 |