Literature summary for 2.1.1.179 extracted from

Nosrati, M.; Dey, D.; Mehrani, A.; Strassler, S.E.; Zelinskaya, N.; Hoffer, E.D.; Stagg, S.M.; Dunham, C.M.; Conn, G.L.
Functionally critical residues in the aminoglycoside resistance-associated methyltransferase RmtC play distinct roles in 30S substrate recognition (2019), J. Biol. Chem., 294, 17642-17653 .

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Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with S-adenosylhomocysteine. An N-terminal domain surface within RmtC, comprising basic residues from both the N1 and N2 subdomains, directly contributes to 30S-binding affinity. Additional residues lining a contiguous adjacent surface on the C-terminal domain are critical for 16S rRNA modification but do not directly contribute to the binding affinity	Proteus mirabilis

Protein Variants

Protein Variants	Comment	Organism
H54A	inactive. Mutation dos not impact 30S binding affinity. Mutant strain is sensitive to kanamycin and gentamicin	Proteus mirabilis
H54E	inactive. Mutation dos not impact 30S binding affinity. Mutant strain is sensitive to kanamycin and gentamicin	Proteus mirabilis
K20E	mutation eliminates 30S binding affinity, mutant strain is sensitive to kanamycin and gentamicin	Proteus mirabilis
K72E	mutation reduces 30S binding affinity about 5fold, resistance to kanamycin and gentamcin is reduced	Proteus mirabilis
additional information	replacement of the RmtC loop with four Ala residues (Loop237-246 ->A4) ablates the enzyme's ability to confer resistance to kanamycin and gentamicin. Conserved C-terminal domain residues surrounding the SAM-binding pocket are functionally critical but do not contribute to 30S binding affinity	Proteus mirabilis
R50E	mutation reduces 30S binding affinity about 11-13fold. Mutant strain is sensitive to kanamycin and gentamicin	Proteus mirabilis
R68E	mutation reduces 30S binding affinity about 11-13fold, resistance to kanamycin and gentamcin is reduced	Proteus mirabilis

Organism

Organism	UniProt	Comment	Textmining
Proteus mirabilis	Q33DX5	-	-

Synonyms

Synonyms	Comment	Organism
RmtC	-	Proteus mirabilis

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Release 2023.1 (January 2023)