BRENDA - Enzyme Database show
show all sequences of 2.1.1.179

Structural bases for 16 S rRNA methylation catalyzed by ArmA and RmtB methyltransferases

Schmitt, E.; Galimand, M.; Panvert, M.; Courvalin, P.; Mechulam, Y.; J. Mol. Biol. 388, 570-582 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Escherichia coli
Crystallization (Commentary)
Crystallization
Organism
RmtB containing S-adenosyl-L-homocysteine in the active site
Escherichia coli
Engineering
Amino acid exchange
Commentary
Organism
E182A
tobramycin MIC is identical with that of wild-type RmtB
Escherichia coli
H50A
tobramycin MIC is drastically reduced compared to wild-type enzyme
Escherichia coli
H81A
tobramycin MIC is identical with that of wild-type RmtB. No change in methylation activity compared to wild-type enzyme
Escherichia coli
K14A
tobramycin MIC is identical with that of wild-type RmtB. 36% of the methylation activity compared to wild-type enzyme
Escherichia coli
K174A
tobramycin MIC is drastically reduced compared to wild-type enzyme. 0.7% of the methylation activity compared to wild-type enzyme
Escherichia coli
R17A
tobramycin MIC is identical with that of wild-type RmtB
Escherichia coli
R181A
tobramycin MIC is drastically reduced compared to wild-type enzyme
Escherichia coli
R48A
tobramycin MIC is identical with that of wild-type RmtB
Escherichia coli
S83A
tobramycin MIC is identical with that of wild-type RmtB. 18% of the methylation activity compared to wild-type enzyme
Escherichia coli
Y56F
tobramycin MIC is identical with that of wild-type RmtB
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + guanine1405 in 16S rRNA
Escherichia coli
post-transcriptional methylation of N7-G1405 in 16S rRNA of 30S ribosomal subunits
S-adenosyl-L-homocysteine + 7-methylguanine1405 in 16S rRNA
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Purification (Commentary)
Commentary
Organism
-
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + guanine1405 in 16S rRNA
-
712741
Escherichia coli
S-adenosyl-L-homocysteine + 7-methylguanine1405 in 16S rRNA
-
-
-
?
S-adenosyl-L-methionine + guanine1405 in 16S rRNA
post-transcriptional methylation of N7-G1405 in 16S rRNA of 30S ribosomal subunits
712741
Escherichia coli
S-adenosyl-L-homocysteine + 7-methylguanine1405 in 16S rRNA
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Crystallization (Commentary) (protein specific)
Crystallization
Organism
RmtB containing S-adenosyl-L-homocysteine in the active site
Escherichia coli
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
E182A
tobramycin MIC is identical with that of wild-type RmtB
Escherichia coli
H50A
tobramycin MIC is drastically reduced compared to wild-type enzyme
Escherichia coli
H81A
tobramycin MIC is identical with that of wild-type RmtB. No change in methylation activity compared to wild-type enzyme
Escherichia coli
K14A
tobramycin MIC is identical with that of wild-type RmtB. 36% of the methylation activity compared to wild-type enzyme
Escherichia coli
K174A
tobramycin MIC is drastically reduced compared to wild-type enzyme. 0.7% of the methylation activity compared to wild-type enzyme
Escherichia coli
R17A
tobramycin MIC is identical with that of wild-type RmtB
Escherichia coli
R181A
tobramycin MIC is drastically reduced compared to wild-type enzyme
Escherichia coli
R48A
tobramycin MIC is identical with that of wild-type RmtB
Escherichia coli
S83A
tobramycin MIC is identical with that of wild-type RmtB. 18% of the methylation activity compared to wild-type enzyme
Escherichia coli
Y56F
tobramycin MIC is identical with that of wild-type RmtB
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + guanine1405 in 16S rRNA
Escherichia coli
post-transcriptional methylation of N7-G1405 in 16S rRNA of 30S ribosomal subunits
S-adenosyl-L-homocysteine + 7-methylguanine1405 in 16S rRNA
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + guanine1405 in 16S rRNA
-
712741
Escherichia coli
S-adenosyl-L-homocysteine + 7-methylguanine1405 in 16S rRNA
-
-
-
?
S-adenosyl-L-methionine + guanine1405 in 16S rRNA
post-transcriptional methylation of N7-G1405 in 16S rRNA of 30S ribosomal subunits
712741
Escherichia coli
S-adenosyl-L-homocysteine + 7-methylguanine1405 in 16S rRNA
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Escherichia coli
Other publictions for EC 2.1.1.179
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733120
Correa
Heterologous expression and fu ...
Escherichia coli, Micromonospora zionensis
Antimicrob. Agents Chemother.
60
699-702
2015
-
-
1
-
-
-
-
-
-
-
3
2
-
3
-
-
1
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
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-
-
1
-
-
-
-
-
-
-
-
-
-
3
2
-
-
-
1
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
735241
Lioy
Aminoglycoside resistance 16S ...
Escherichia coli, Escherichia coli MM294
RNA
20
382-391
2014
-
-
-
-
-
-
1
-
-
-
-
3
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
735238
Gutierrez
Indigenous and acquired modifi ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa BB1285
RNA Biol.
10
1324-1332
2013
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
2
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-
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-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
718596
Gutierrez
Fitness cost and interference ...
Escherichia coli, Escherichia coli BW25113
Antimicrob. Agents Chemother.
56
2335-2341
2012
-
-
1
-
1
-
-
-
-
-
-
4
-
31
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
718603
Galimand
RmtF, a new member of the amin ...
Klebsiella pneumoniae, Klebsiella pneumoniae BM4686
Antimicrob. Agents Chemother.
56
3960-3962
2012
-
-
1
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
719353
Wachino
Exogenously acquired 16S rRNA ...
Citrobacter freundii, Micromonospora echinospora, Micromonospora inyonensis, Micromonospora olivasterospora, Micromonospora rosea, Micromonospora zionensis, Proteus mirabilis, Proteus mirabilis ARS68, Pseudomonas aeruginosa, Serratia marcescens, Streptoalloteichus hindustanus, Streptomyces kanamyceticus
Drug Resist. Updat.
15
133-148
2012
-
11
11
-
-
-
-
-
-
-
-
13
-
21
-
-
-
-
-
-
-
-
13
-
-
-
-
-
-
-
-
11
-
-
-
-
12
12
12
-
-
-
-
-
-
-
-
-
-
13
-
-
-
-
-
-
-
-
13
-
-
-
-
-
-
-
-
-
-
22
24
-
-
-
705988
Husain
Structural basis for the methy ...
Micromonospora zionensis
Nucleic Acids Res.
38
4120-4132
2010
-
-
1
1
-
-
-
-
-
-
-
1
-
4
-
-
1
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
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-
1
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
712038
Wachino
RmtC introduces G1405 methylat ...
Proteus mirabilis, Proteus mirabilis ARS68
FEMS Microbiol. Lett.
311
56-60
2010
-
-
1
-
-
-
-
-
-
-
-
2
-
4
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
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-
2
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
705972
Savic
Determination of the target nu ...
Frankia sp., Frankia sp. CcI3, Micromonospora echinospora, Micromonospora zionensis
Nucleic Acids Res.
37
5420-5431
2009
-
-
-
-
-
-
-
-
-
-
-
4
-
4
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
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-
-
4
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
706724
Cubrilo
The aminoglycoside resistance ...
Micromonospora zionensis
RNA
15
1492-1497
2009
-
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
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-
1
-
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-
-
-
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-
1
-
-
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-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
712741
Schmitt
Structural bases for 16 S rRNA ...
Escherichia coli
J. Mol. Biol.
388
570-582
2009
-
-
1
1
10
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
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-
1
-
1
10
-
-
-
-
-
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-
1
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-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
702415
Maravic Vlahovicek
Modeling and experimental anal ...
Micromonospora zionensis
Biochim. Biophys. Acta
1784
582-590
2008
-
-
1
-
14
-
-
-
-
-
-
-
-
2
-
-
1
1
-
-
-
-
1
-
-
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-
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-
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-
1
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-
14
-
-
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-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
704280
Savic
Critical residues for cofactor ...
Micromonospora zionensis
J. Bacteriol.
190
5855-5861
2008
-
-
1
-
7
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
706701
Tomic
Aminoglycoside resistance gene ...
Micromonospora zionensis
Res. Microbiol.
159
658-662
2008
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
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-
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1
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1
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
710881
Périchon
Transferable resistance to ami ...
plasmid pIP1206
Antimicrob. Agents Chemother.
51
2464-2469
2007
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
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-
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1
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1
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1
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
710880
Wachino
Novel plasmid-mediated 16S rRN ...
Proteus mirabilis, Proteus mirabilis ARS68
Antimicrob. Agents Chemother.
50
178-184
2006
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
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1
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1
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-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
712738
Liou
Aminoglycoside resistance by A ...
plasmid pAT780
J. Mol. Biol.
359
358-364
2006
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
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-
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-
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-
-
-
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-
-
-
-
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-
-
1
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
704254
Kojic
Translational autoregulation o ...
Micromonospora zionensis
J. Bacteriol.
178
5493-5498
1996
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
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2
-
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-
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-
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-
-
-
-
-
-
-
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-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
704248
Kojic
Cloning and characterization o ...
Micromonospora zionensis
J. Bacteriol.
174
7868-7872
1992
-
-
1
-
-
-
-
-
-
-
1
-
-
5
-
-
-
-
-
-
-
-
-
1
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-
-
-
-
-
-
-
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1
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1
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-
1
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-
-
-
-
-
-
-
-
-
-
-
-
-
705105
Beauclerk
Sites of action of two ribosom ...
Micromonospora echinospora
J. Mol. Biol.
193
661-671
1987
-
-
-
-
-
-
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1
-
1
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2
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1
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-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-