EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
3.1.1.6 | 6.9 |
- |
tributyrin |
mutant enzyme R48K, in 20 mM phosphate buffer (pH 7.1), at 30°C |
715950 |
3.1.1.6 | 7 |
- |
2-t-butylcyclohex-1-en-1-yl acetate |
isoenzyme Est I, pH 7.0, 35°C |
666089 |
3.1.1.6 | 9.3 |
- |
tributyrin |
mutant enzyme R48E, in 20 mM phosphate buffer (pH 7.1), at 30°C |
715950 |
3.1.1.6 | 12.6 |
- |
6-acetylmorphine |
recombinant enzyme, pH 8.0 |
652394 |
3.1.1.6 | 14 |
- |
2-isopropylcyclohex-1-en-1-yl acetate |
isoenzyme Est I, pH 7.0, 35°C |
666089 |
3.1.1.6 | 17 |
- |
2-t-butylcyclohex-1-en-1-yl acetate |
isoenzyme Est II, pH 7.0, 35°C |
666089 |
3.1.1.6 | 17.4 |
- |
tributyrin |
mutant enzyme R48A, in 20 mM phosphate buffer (pH 7.1), at 30°C |
715950 |
3.1.1.6 | 17.5 |
- |
tributyrin |
mutant enzyme R48S, in 20 mM phosphate buffer (pH 7.1), at 30°C |
715950 |
3.1.1.6 | 20 |
- |
2-benzylcyclohex-1-en-1-yl acetate |
isoenzyme Est I, pH 7.0, 35°C |
666089 |
3.1.1.6 | 21 |
- |
4-nitrophenyl-butanoate |
pH 7.1, 25°C, recombinant wild-type enzyme |
682718 |