3.1.1.6 additional information - 4-nitrophenyl-beta-D-xylopyranoside monoacetate initial rate ratio between 2-, 3-, and 4-O-acetyl 4-nitrophenyl-beta-D-xylopyranoside is 1:19:17.7 695758 3.1.1.6 0.15 - 4-nitrophenyl-2-O-acetyl-alpha-L-arabinofuranoside pH 5.5, 45°C 680967 3.1.1.6 0.82 - 4-nitrophenyl acetate at pH 7.0 and 39°C 752328 3.1.1.6 0.91 - 4-nitrophenyl-4-O-acetyl-beta-D-xylopyranoside pH 5.5, 45°C 680967 3.1.1.6 1.77 - 4-nitrophenyl-5-O-acetyl-alpha-L-arabinofuranoside pH 5.5, 45°C 680967 3.1.1.6 1.9 - 4-nitrophenyl-3-O-acetyl-beta-D-xylopyranoside pH 5.5, 45°C 680967 3.1.1.6 3 - phenyl acetate recombinant enzyme, pH 6.4 652394 3.1.1.6 3.23 - 4-nitrophenyl-2-O-acetyl-beta-D-xylopyranoside pH 5.5, 45°C 680967 3.1.1.6 5.5 - 4-nitrophenyl acetate at pH 8.0 and 20°C 750119 3.1.1.6 6.2 - tributyrin wild type enzyme, in 20 mM phosphate buffer (pH 7.1), at 30°C 715950 3.1.1.6 6.9 - tributyrin mutant enzyme R48K, in 20 mM phosphate buffer (pH 7.1), at 30°C 715950 3.1.1.6 7 - 2-t-butylcyclohex-1-en-1-yl acetate isoenzyme Est I, pH 7.0, 35°C 666089 3.1.1.6 9.3 - tributyrin mutant enzyme R48E, in 20 mM phosphate buffer (pH 7.1), at 30°C 715950 3.1.1.6 12.6 - 6-acetylmorphine recombinant enzyme, pH 8.0 652394 3.1.1.6 14 - 2-isopropylcyclohex-1-en-1-yl acetate isoenzyme Est I, pH 7.0, 35°C 666089 3.1.1.6 17 - 2-t-butylcyclohex-1-en-1-yl acetate isoenzyme Est II, pH 7.0, 35°C 666089 3.1.1.6 17.4 - tributyrin mutant enzyme R48A, in 20 mM phosphate buffer (pH 7.1), at 30°C 715950 3.1.1.6 17.5 - tributyrin mutant enzyme R48S, in 20 mM phosphate buffer (pH 7.1), at 30°C 715950 3.1.1.6 20 - 2-benzylcyclohex-1-en-1-yl acetate isoenzyme Est I, pH 7.0, 35°C 666089 3.1.1.6 21 - 4-nitrophenyl-butanoate pH 7.1, 25°C, recombinant wild-type enzyme 682718 3.1.1.6 21.8 - 4-nitrophenyl acetate mutant enzyme S10A, at pH 5.5 and 50°C 750537 3.1.1.6 26 - 2-n-pentylcyclohex-1-en-1-yl acetate isoenzyme Est II, pH 7.0, 35°C 666089 3.1.1.6 26.2 - 4-nitrophenyl acetate mutant enzyme C16A, at pH 5.5 and 50°C 750537 3.1.1.6 29 - 4-nitrophenyl butyrate mutant enzyme R48K, in 20 mM phosphate buffer (pH 7.1), at 30°C 715950 3.1.1.6 29 - 4-nitrophenyl butyrate wild type enzyme, in 20 mM phosphate buffer (pH 7.1), at 30°C 715950 3.1.1.6 29 - 4-nitrophenyl butyrate wild type enzyme, in 20 mM phosphate buffer, at pH 7.1 and 30°C 729428 3.1.1.6 29.8 - 4-nitrophenyl butyrate wild type enzyme, at pH 5.5 and 50°C 750537 3.1.1.6 33 - 2-benzylcyclohex-1-en-1-yl acetate isoenzyme Est II, pH 7.0, 35°C 666089 3.1.1.6 34.5 - 4-nitrophenyl hexanoate pH 7.1, 65°C, recombinant wild-type enzyme 682718 3.1.1.6 36.3 - 4-nitrophenyl acetate mutant enzyme D179A, at pH 5.5 and 50°C 750537 3.1.1.6 37 - 4-nitrophenyl butyrate mutant enzyme L209F, in 20 mM phosphate buffer, at pH 7.1 and 30°C 729428 3.1.1.6 38.8 - 4-nitrophenyl acetate mutant enzyme H182A, at pH 5.5 and 50°C 750537 3.1.1.6 41.3 - 4-nitrophenyl propionate in 50 mM citrate-phosphate (pH 6) at 70°C 730907 3.1.1.6 41.6 - 2-O-acetyl 4-nitrophenyl beta-D-xylopyranoside isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C 715029 3.1.1.6 42 - 2-n-pentylcyclohex-1-en-1-yl acetate isoenzyme Est I, pH 7.0, 35°C 666089 3.1.1.6 48.8 - 4-nitrophenyl hexanoate pH 7.1, 65°C, recombinant mutant T74A 682718 3.1.1.6 49.2 - 4-nitrophenyl acetate wild type enzyme, at pH 5.5 and 50°C 750537 3.1.1.6 57.5 - 4-nitrophenyl acetate in 50 mM citrate-phosphate (pH 6) at 70°C 730907 3.1.1.6 63 - 2-ethylcyclohex-1-en-1-yl acetate isoenzyme Est I, pH 7.0, 35°C 666089 3.1.1.6 70.1 - 3-O-acetyl-4-nitrophenyl beta-D-xylopyranoside in 50 mM citrate-phosphate (pH 6) at 70°C 730907 3.1.1.6 76.1 - 2-O-acetyl-4-nitrophenyl beta-D-xylopyranoside in 50 mM citrate-phosphate (pH 6) at 70°C 730907 3.1.1.6 77 - 2-methylcyclohex-1-en-1-yl acetate isoenzyme Est I, pH 7.0, 35°C 666089 3.1.1.6 78 - 4-nitrophenyl butyrate mutant enzyme R48E, in 20 mM phosphate buffer (pH 7.1), at 30°C 715950 3.1.1.6 78.6 - 4-O-acetyl-4-nitrophenyl beta-D-xylopyranoside in 50 mM citrate-phosphate (pH 6) at 70°C 730907 3.1.1.6 79 - 2-n-propylcyclohex-1-en-1-yl acetate isoenzyme Est I, pH 7.0, 35°C 666089 3.1.1.6 86 - 4-nitrophenyl butyrate mutant enzyme R48S, in 20 mM phosphate buffer (pH 7.1), at 30°C 715950 3.1.1.6 113 - 4-nitrophenyl butyrate mutant enzyme R48A, in 20 mM phosphate buffer (pH 7.1), at 30°C 715950 3.1.1.6 145 - 4-nitrophenyl-butanoate pH 7.1, 65°C, recombinant wild-type enzyme 682718 3.1.1.6 155 - 4-nitrophenyl-butanoate pH 7.1, 65°C, recombinant mutant T74A 682718 3.1.1.6 240 - 4-nitrophenyl butyrate mutant enzyme L97F, in 20 mM phosphate buffer, at pH 7.1 and 30°C 729428 3.1.1.6 241.6 - 4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside isozyme CE2C, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C 715029 3.1.1.6 340 - 4-nitrophenyl butyrate mutant enzyme L97F/L209F, in 20 mM phosphate buffer, at pH 7.1 and 30°C 729428 3.1.1.6 1331 - 4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside isozyme CE2, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C 715029 3.1.1.6 2101 - 3-O-acetyl 4-nitrophenyl beta-D-xylopyranoside isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C 715029 3.1.1.6 3210 - 4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C 715029