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(4R)-hydroxy-3-methyl-2-(2-propynyl)-cyclopent-2-enone acetate + H2O
?
(acetyloxy)(phenyl)acetic acid + H2O
(S)-mandelic acid + (2R)-(acetyloxy)(phenyl)ethanoic acid
(R)-1,2-O-isopropylidene glycol acetate + H2O
(R)-1,2-O-isopropylidene glycol + acetate
-
-
-
-
?
(R,S)-1,2-O-isopropylidene glycol butyrate + H2O
(R)-1,2-O-isopropylidene glycol + butyrate
-
fresh bacterial cells or purified EST1 (0.01 micromol p-nitrophenol/min), 28°C, pH 8, 5% (v/v) glycerol, 1 mM EDTA, 1 mM dithiothreitol
low enantioselectivity (enantiomeric ratio E: 2.5 for EST1, E: 2.5 for whole cells)
-
?
(R,S)-1,2-O-isopropylidene glycol caproate + H2O
(R)-1,2-O-isopropylidene glycol + caproate
-
fresh bacterial cells or purified EST1 (0.01 micromol p-nitrophenol/min), 28°C, pH 8, 5% (v/v) glycerol, 1 mM EDTA, 1 mM dithiothreitol
no enantioselectivity by EST1 (enantiomeric ratio E: 1) compared to E: 2.8 for whole cells
-
?
1-naphthol acetate + H2O
1-naphthol + acetate
1-naphthyl acetate + H2O
1-naphthol + acetate
1-naphthyl butyrate + H2O
1-naphthol + butyrate
36% activity compared to 1-naphthyl acetate
-
-
?
1-phenyl-1-(trifluoromethyl)prop-2-yn-1-yl acetate + H2O
1-phenyl-1-(trifluoromethyl)prop-2-yn-1-ol + acetate
-
determination of enantiopreference, wild-type R enantioselectivity is 42
spectrophotometric assay coupling released acetate to NADH generation
-
?
13-(6,6'-diacetyl)sophorosyloxydocosanoic acid + H2O
?
Candida bororiensis
-
-
-
-
?
2-benzylcyclohex-1-en-1-yl acetate + H2O
2-benzylcyclohexanone + acetate
2-ethylcyclohex-1-en-1-yl acetate + H2O
2-ethylcyclohexanone + acetate
-
isoenzyme Est I, 99% conversion of substrate with 99% S-configuration of product
-
-
?
2-isopropylcyclohex-1-en-1-yl acetate + H2O
2-isopropylcyclohexanone + acetate
-
isoenzyme Est I, 10% conversion of substrate with R-configuration of product
-
-
?
2-methylcyclohex-1-en-1-yl acetate + H2O
(S)-2-methylcyclohexanone + acetate
-
isoenzyme Est I, 99% conversion of substrate with 99% S-configuration of product
-
-
?
2-n-pentylcyclohex-1-en-1-yl acetate + H2O
2-n-pentylcyclohexanone + acetate
2-n-propylcyclohex-1-en-1-yl acetate + H2O
2-n-propylcyclohexanone + acetate
-
isoenzyme Est I, 99% conversion of substrate with 99% R-configuration of product
-
-
?
2-naphthol acetate + H2O
2-naphthol + acetate
2-naphthyl acetate + H2O
2-naphthol + acetate
2-O-acetyl 4-nitrophenyl beta-D-xylopyranoside + H2O
?
2-O-acetyl-4-nitrophenyl beta-D-xylopyranoside + H2O
?
-
-
-
-
?
2-t-butylcyclohex-1-en-1-yl acetate + H2O
2-t-butylcyclohexanone + acetate
3,6-diacetylmorphine + H2O
morphine + acetate
3-O-acetyl 4-nitrophenyl beta-D-xylopyranoside + H2O
?
3-O-acetyl-4-nitrophenyl beta-D-xylopyranoside + H2O
?
-
-
-
-
?
3-O-methyl-D-glucopyranoside + vinyl acetate
6-O-acetyl-3-O-methyl-D-glucopyranoside + vinyl alcohol
4-acetoxybenzoic acid + H2O
4-hydroxybenzoate + acetate
-
-
-
-
?
4-methylumbelliferone + H2O
?
-
-
-
-
?
4-methylumbelliferyl acetate + H2O
4-methylumbelliferone + acetate
-
-
-
?
4-methylumbelliferyl acetate + H2O
?
4-nitrophenyl 2-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl-beta-D-xylopyranoside + acetate
4-nitrophenyl 3-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl-beta-D-xylopyranoside + acetate
-
-
-
-
?
4-nitrophenyl 4-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl-beta-D-xylopyranoside + acetate
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
4-nitrophenyl beta-D-glucopyranoside + vinyl acetate
4-nitrophenyl 3-O-acetyl-beta-D-glucopyranoside + vinyl alcohol
-
yield is 70.2%
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenyl D-xylopyranose
4 mM substrate, position-specificity, hydrolysis activity is specific for 3-, and 4-O-acetyl 4-nitrophenyl-beta-D-xylopyranoside, 2-O-acetyl 4-nitrophenyl-beta-D-xylopyranoside is a bad substrate
beta-xylosidase-coupled assay
-
?
4-nitrophenyl butyrate + H2O
4-nitrophenol + butyrate
4-nitrophenyl formate + H2O
4-nitrophenol + formate
5 min, 30°C, pH 5.8
-
-
?
4-nitrophenyl hexanoate + H2O
4-nitrophenol + hexanoate
-
-
-
-
?
4-nitrophenyl propionate + H2O
4-nitrophenol + propionate
4-nitrophenyl-2-O-acetyl-alpha-L-arabinofuranoside + H2O
4-nitrophenyl-alpha-L-arabinofuranoside + acetate
-
-
-
-
?
4-nitrophenyl-2-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl-beta-D-xylopyranoside + acetate
-
preferred substrate
-
-
?
4-nitrophenyl-3-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl-beta-D-xylopyranoside + acetate
-
-
-
-
?
4-nitrophenyl-4-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl-beta-D-xylopyranoside + acetate
-
-
-
-
?
4-nitrophenyl-5-O-acetyl-alpha-L-arabinofuranoside + H2O
4-nitrophenyl-alpha-L-arabinofuranoside + acetate
-
-
-
-
?
4-nitrophenyl-butanoate + H2O
4-nitrophenol + butanoate
-
-
-
-
?
4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside + H2O
?
4-O-acetyl-4-nitrophenyl beta-D-xylopyranoside + H2O
?
-
-
-
-
?
6-acetylmorphine + H2O
morphine + acetate
6-O-acetyl-D-mannopyranose + H2O
?
-
-
-
-
?
6-O-acetyl-D-mannopyranoside + H2O
?
-
-
-
-
?
7-aminocephalosporanic acid + H2O
?
-
-
-
-
?
7-aminocephalosporanic acid + H2O
deacetylated 7-aminocephalosporanic acid + acetate
-
-
-
-
?
9-O-acetyl sialic acid + H2O
sialic acid + acetate
-
-
-
?
acetyl alginate + H2O
alginate + acetate
-
-
-
?
acetyl xylan + H2O
xylan + acetate
-
from beech wood or oat spelt
-
?
acetylated hemicellulose + H2O
? + acetate
-
the best substrate of the enzyme is 3-O-acetylated Xylp residue followed by 4-O-acetylated Xylp residue with a free vicinal hydroxyl group
-
-
?
acetylglucuronoxylan + H2O
glucuronoxylan + acetate
-
the enzyme deacetylates singly and doubly acetylated xylopyranosyl residues in the polymer to such an extent that it results in the polymer precipitation
-
-
?
acetylhemicellulose + H2O
hemicellulose + acetate
-
-
-
-
?
acetylxylan + H2O
xylan + acetate
-
60°C, pH 6, acetyl xylan esterase (acetate-releasing) activity on birchwood xylan
-
-
?
alpha,alpha-trehalose + vinyl acetate
? + vinyl alcohol
-
-
-
-
?
alpha-naphthyl acetate + H2O
alpha-naphthol + acetate
an acetic ester + H2O
an alcohol + acetate
-
-
-
-
?
apigenin 7-O-(6-O-malonylglucoside) + H2O
?
-
-
-
-
?
arabinogalactan + H2O
?
-
-
-
-
?
astragaloside I + H2O
astragaloside IV + ?
astragaloside II + H2O
astragaloside IV + ?
beechwood xylan + H2O
?
-
-
-
-
?
beta-naphthyl acetate + H2O
beta-naphthol + acetate
birchwood xylan + H2O
?
-
-
-
-
?
bovine submaxillary mucin + H2O
deacetylated bovine submaxillary mucin + acetate
-
-
-
?
cellobiose + vinyl acetate
? + vinyl alcohol
cellobiose octaacetate + H2O
?
-
low activity
-
?
cellulose acetate + H2O
cellulose + acetate
cephalosporin C + H2O
deacetylated cephalosporin C + acetate
-
-
-
-
?
cephalosporin C + H2O
deacetylcephalosporin C + acetate
-
-
-
-
?
chlorogenic acid + H2O
?
-
-
-
-
?
D-galactose + vinyl acetate
? + vinyl alcohol
D-glucopyranosyl beta-(1,3)-xylopyranoside + vinyl acetate
? + vinyl alcohol
D-glucopyranosyl beta-(1,4)-xylopyranoside + vinyl acetate
? + vinyl alcohol
D-glucose + vinyl acetate
6-O-acetyl-D-glucopyranose + vinyl alcohol
D-glucose penta-acetate + H2O
?
D-mannose + vinyl acetate
6-O-acetyl-D-mannopyranose + vinyl alcohol
D-xylopyranosyl beta-(1,4)-D-glucopyranoside + vinyl acetate
? + vinyl alcohol
diacetin + H2O
?
-
-
-
-
?
ethyl acetate + H2O
ethanol + acetate
geraniol acetate + H2O
geraniol + acetate
-
enzyme plays a role in geraniol production and improving palmarosa oil quality during florescence development
-
?
geranyl acetate + H2O
geraniol + acetate
-
-
-
?
glucose penta-acetate + H2O
?
-
high activity
-
-
?
glucose pentaacetate + H2O
?
glycerol triacetate + H2O
?
isoamyl acetate + H2O
isoamyl alcohol + acetate
-
25°C, 1 h, pH 7, 7 mM MgCl2, cell extracts
-
-
?
isoastragaloside I + H2O
astragaloside IV + ?
isoastragaloside II + H2O
astragaloside IV + ?
kojic acid + vinyl acetate
7-O-acetyl-kojic acid + vinyl alcohol
-
yield is 30.9%
-
-
?
malonylated isorhamnetin 3-O-glucoside + H2O
?
-
-
-
-
?
methyl 2,3,4-O-triacetyl-beta-D-xylopyranoside + 3 H2O
methyl beta-D-xylopyranoside + 3 acetate
-
all four acetylated derivatives of methyl beta-xylopyranoside are completely deacetylated after 24 h
-
-
?
methyl 2,3,4-O-triacetyl-beta-D-xylopyranoside + H2O
methyl 3,4-O-diacetyl-beta-D-xylopyranoside + acetate
-
-
-
-
?
methyl 2,3-O-diacetyl-beta-D-xylopyranoside + 2 H2O
methyl beta-D-xylopyranoside + 2 acetate
-
all four acetylated derivatives of methyl beta-xylopyranoside are completely deacetylated after 24 h
-
-
?
methyl 2,3-O-diacetyl-beta-D-xylopyranoside + H2O
?
methyl 2,3-O-diacetyl-beta-D-xylopyranoside + H2O
methyl 2-O-acetyl-beta-D-xylopyranoside + acetate
-
-
-
-
?
methyl 2,4-O-diacetyl-beta-D-xylopyranoside + 2 H2O
methyl beta-D-xylopyranoside + 2 acetate
-
all four acetylated derivatives of methyl beta-xylopyranoside are completely deacetylated after 24 h
-
-
?
methyl 2,4-O-diacetyl-beta-D-xylopyranoside + H2O
?
methyl 2-O-acetyl-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranoside + H2O
methyl xylotriose + acetate
-
-
-
-
?
methyl 2-O-methyl-beta-D-glucopyranoside + vinyl acetate
? + vinyl alcohol
methyl 3,4-O-diacetyl-beta-D-xylopyranoside + 2 H2O
methyl beta-D-xylopyranoside + 2 acetate
-
all four acetylated derivatives of methyl beta-xylopyranoside are completely deacetylated after 24 h
-
-
?
methyl 3,4-O-diacetyl-beta-D-xylopyranoside + H2O
?
methyl 3-O-acetyl-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranoside + H2O
methyl xylotriose + acetate
-
-
-
-
?
methyl 4-O-acetyl-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranoside + H2O
methyl xylotriose + acetate
-
-
-
-
?
methyl 4-O-methyl-alpha-D-glucopyranoside + vinyl acetate
? + vinyl alcohol
methyl 6-O-acetyl-beta-D-glucopyranoside + H2O
?
methyl acetate + H2O
methanol + acetate
methyl alpha-D-glucopyranoside + vinyl acetate
? + vinyl alcohol
methyl beta-D-glucopyranoside + vinyl acetate
? + vinyl alcohol
methyl beta-D-glucopyranoside + vinyl acetate
methyl 3-O-acetyl-beta-D-glucopyranoside + vinyl alcohol
-
yield is 56.4%
-
-
?
methyl-beta-D-xylopyranoside + vinylacetate
methyl-beta-D-xylopyranoside monoacetate + vinyl alcohol
transacetylation of methylxyloside in aqueous solution (40°C, pH 6, 25 mM methyl-beta-D-xylopyranoside)
analysis by thin-layer chromatography
-
?
monoacetin + H2O
glycerol + acetate
-
-
-
-
?
naphthyl acetate + H2O
naphthol + acetate
-
-
-
-
?
naphthyl butyrate + H2O
naphthol + butyrate
-
-
-
-
?
naphthyl propionate + H2O
naphthol + propionate
-
-
-
-
?
O-acetyl-1,4-beta-xylooligosaccharide + H2O
1,4-beta-xylooligosaccharide + acetic acid
O-acetyl-4-O-methyl-D-glucurono-D-xylan + H2O
4-O-methyl-D-glucurono-D-xylan + acetic acid
O-acetyl-galactoglucomannan + H2O
?
-
-
-
-
?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
p-nitrophenyl butyrate + H2O
p-nitrophenol + butyrate
-
-
-
-
?
p-nitrophenyl decanoate + H2O
p-nitrophenol + decanoate
-
-
-
-
?
p-nitrophenyl hexanoate + H2O
p-nitrophenol + hexanoate
p-nitrophenyl laurate + H2O
p-nitrophenol + laurate
p-nitrophenyl propionate + H2O
p-nitrophenol + propionate
penta-O-acetyl-alpha-D-glucose + H2O
?
-
complete and regioselective removal of primary acetyl-group in 6-position
-
-
?
phenyl acetate + H2O
phenol + acetate
poly(vinylacetate) + H2O
? + acetate
-
-
-
-
?
polygalacturonan + H2O
?
-
-
-
-
?
potato pectin + H2O
? + acetate
-
-
-
-
?
racemic 1-phenylethyl acetate + H2O
(1R)-phenylethanol + (1S)-phenylethanol + acetate
-
-
-
-
?
sugar beet pectin + H2O
? + acetate
-
highest activity
-
-
?
tert-butyl acetate + H2O
2-methylpropane + acetate
-
-
-
?
tobacco hemicellulose + H2O
?
-
lowest activity
-
-
?
triacetin + H2O
?
-
EST1, pH 8, 20°C, 0.5% (w/v) Triton X-100, 0.125% (w/v) Arabic gum
titration assay
-
?
triacetine + H2O
?
-
-
-
-
?
tripropionin + H2O
?
-
-
-
-
?
xylobiose + vinyl acetate
? + vinyl alcohol
xylose tetraacetate + H2O
?
[(4S)-2,2-dimethyl-1,3-dioxolan-4-yl]methyl acetate + H2O
[(4R)-2,2-dimethyl-1,3-dioxolan-4-yl]methanol + acetate
-
fresh bacterial cells or purified enzyme (EST1 or EST2, 0.01 micromol p-nitrophenol/min), 20°C, pH 8, 5% (v/v) glycerol, 1 mM EDTA, 1 mM dithiothreitol
high enantioselectivity (enantiomeric ratio E: 20 for EST1, E: 15 for whole cells), thin-layer chromatography, enantiomeric composition of reactants controlled by gas chromatography, acetate detection spectrophotometrically through NADH absorbance at 340 nm
-
?
additional information
?
-
(4R)-hydroxy-3-methyl-2-(2-propynyl)-cyclopent-2-enone acetate + H2O

?
-
highly enantioselective enzyme, enantioselectivity raises by 1.2fold from 16 to 20 when reaction temperature is raised from 30°C to 60°C. Both enantioselectivity and activity are raised by addition of the cationic detergent benzethonium chloride
-
-
?
(4R)-hydroxy-3-methyl-2-(2-propynyl)-cyclopent-2-enone acetate + H2O
?
-
highly enantioselective enzyme, enantioselectivity raises by 1.2fold from 16 to 20 when reaction temperature is raised from 30°C to 60°C. Both enantioselectivity and activity are raised by addition of the cationic detergent benzethonium chloride
-
-
?
(acetyloxy)(phenyl)acetic acid + H2O

(S)-mandelic acid + (2R)-(acetyloxy)(phenyl)ethanoic acid