Any feedback?
Information on EC 3.1.1.6 - acetylesterase
for references in articles please use BRENDA:EC3.1.1.6Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
3.1.1.6 acetylesteraseSpecify your search results
Word Map
- 3.1.1.6
- esterases
- xylans
-
deacetylation
-
saccharification
-
carbohydrate-active
-
glucuronoyl
- isavs
-
receptor-destroying
- acetylxylan
-
cazymes
- glucuronoxylans
-
alpha-naphthyl
- arabinoxylans
-
methylesterase
-
lignin-carbohydrate
- arylesterase
-
feruloylated
-
hemicellulolytic
-
4-methylumbelliferyl
- hemagglutinin-esterase
- xylooligosaccharides
-
beta-naphthyl
-
9-o-acetylated
-
xylopyranosyl
- orthomyxoviridae
- molecular biology
- synthesis
- industry
- paper production
- analysis
The enzyme appears in viruses and cellular organisms
Synonyms
carbohydrate esterase, acetylesterase, est-4, haemagglutinin-esterase, pectin acetylesterase, est24, acetyl-esterase, tm0077, c20orf3, p-nitrophenyl acetate esterase, 
more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
6-O-deacetylase
ACE
acetate ester-hydrolysing esterase
Acetic ester hydrolase
-
-
-
-
Acetyl esterase
acetyl xylan esterase
acetylesterase CE16
AE
Aes1
Axe3
C-esterase
-
-
-
-
carbohydrate acetylesterase
carbohydrate esterase
CE16 acetyl esterase
CE16A
Chloroesterase
-
-
-
-
Citrus acetylesterase
-
-
-
-
Est1
EST2
HerE
p-nitrophenyl acetate esterase
-
-
-
-
PAE1
pectin acetylesterase
ybaC
additional information
-
the enzyme belongs to the carbohydrate esterase family 1
acetate ester-hydrolysing esterase
-
ethyl acetate- and isoamyl acetate-hydrolysing esterase activities
carbohydrate esterase
new family, less than 30% identity to carbohydrate esterases of known families
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
an acetic ester + H2O = an alcohol + acetate
-
-
-
-
an acetic ester + H2O = an alcohol + acetate
active site serine, active site structure, reaction and ligand binding mechanism
-
an acetic ester + H2O = an alcohol + acetate
active site serine, active site structure, reaction and ligand binding mechanism
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
acetylation
hydrolysis of carboxylic ester
transacetylation
hydrolysis of carboxylic ester
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PATHWAY SOURCE
PATHWAYS
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
acetic-ester acetylhydrolase
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CAS REGISTRY NUMBER
COMMENTARY
9000-82-2
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(acetyloxy)(phenyl)acetic acid + H2O
(S)-mandelic acid + (2R)-(acetyloxy)(phenyl)ethanoic acid
(R)-1,2-O-isopropylidene glycol acetate + H2O
(R)-1,2-O-isopropylidene glycol + acetate
-
-
-
-
?
(R,S)-1,2-O-isopropylidene glycol butyrate + H2O
(R)-1,2-O-isopropylidene glycol + butyrate
-
fresh bacterial cells or purified EST1 (0.01 micromol p-nitrophenol/min), 28°C, pH 8, 5% (v/v) glycerol, 1 mM EDTA, 1 mM dithiothreitol
low enantioselectivity (enantiomeric ratio E: 2.5 for EST1, E: 2.5 for whole cells)
-
?
(R,S)-1,2-O-isopropylidene glycol caproate + H2O
(R)-1,2-O-isopropylidene glycol + caproate
-
fresh bacterial cells or purified EST1 (0.01 micromol p-nitrophenol/min), 28°C, pH 8, 5% (v/v) glycerol, 1 mM EDTA, 1 mM dithiothreitol
no enantioselectivity by EST1 (enantiomeric ratio E: 1) compared to E: 2.8 for whole cells
-
?
1-naphthyl butyrate + H2O
1-naphthol + butyrate
36% activity compared to 1-naphthyl acetate
-
-
?
1-phenyl-1-(trifluoromethyl)prop-2-yn-1-yl acetate + H2O
1-phenyl-1-(trifluoromethyl)prop-2-yn-1-ol + acetate
-
determination of enantiopreference, wild-type R enantioselectivity is 42
spectrophotometric assay coupling released acetate to NADH generation
-
?
2-ethylcyclohex-1-en-1-yl acetate + H2O
2-ethylcyclohexanone + acetate
-
isoenzyme Est I, 99% conversion of substrate with 99% S-configuration of product
-
-
?
2-isopropylcyclohex-1-en-1-yl acetate + H2O
2-isopropylcyclohexanone + acetate
-
isoenzyme Est I, 10% conversion of substrate with R-configuration of product
-
-
?
2-methylcyclohex-1-en-1-yl acetate + H2O
(S)-2-methylcyclohexanone + acetate
-
isoenzyme Est I, 99% conversion of substrate with 99% S-configuration of product
-
-
?
2-n-propylcyclohex-1-en-1-yl acetate + H2O
2-n-propylcyclohexanone + acetate
-
isoenzyme Est I, 99% conversion of substrate with 99% R-configuration of product
-
-
?
3-O-methyl-D-glucopyranoside + vinyl acetate
6-O-acetyl-3-O-methyl-D-glucopyranoside + vinyl alcohol
4-methylumbelliferyl acetate + H2O
4-methylumbelliferone + acetate
-
-
-
?
4-nitrophenyl 2-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl-beta-D-xylopyranoside + acetate
4-nitrophenyl 3-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl-beta-D-xylopyranoside + acetate
-
-
-
-
?
4-nitrophenyl 4-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl-beta-D-xylopyranoside + acetate
-
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + vinyl acetate
4-nitrophenyl 3-O-acetyl-beta-D-glucopyranoside + vinyl alcohol
-
yield is 70.2%
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenyl D-xylopyranose
4 mM substrate, position-specificity, hydrolysis activity is specific for 3-, and 4-O-acetyl 4-nitrophenyl-beta-D-xylopyranoside, 2-O-acetyl 4-nitrophenyl-beta-D-xylopyranoside is a bad substrate
beta-xylosidase-coupled assay
-
?
4-nitrophenyl formate + H2O
4-nitrophenol + formate
5 min, 30°C, pH 5.8
-
-
?
4-nitrophenyl-2-O-acetyl-alpha-L-arabinofuranoside + H2O
4-nitrophenyl-alpha-L-arabinofuranoside + acetate
-
-
-
-
?
4-nitrophenyl-2-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl-beta-D-xylopyranoside + acetate
-
preferred substrate
-
-
?
4-nitrophenyl-3-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl-beta-D-xylopyranoside + acetate
-
-
-
-
?
4-nitrophenyl-4-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl-beta-D-xylopyranoside + acetate
-
-
-
-
?
4-nitrophenyl-5-O-acetyl-alpha-L-arabinofuranoside + H2O
4-nitrophenyl-alpha-L-arabinofuranoside + acetate
-
-
-
-
?
7-aminocephalosporanic acid + H2O
deacetylated 7-aminocephalosporanic acid + acetate
-
-
-
-
?
acetyl xylan + H2O
xylan + acetate
-
from beech wood or oat spelt
-
?
acetylated hemicellulose + H2O
? + acetate
-
the best substrate of the enzyme is 3-O-acetylated Xylp residue followed by 4-O-acetylated Xylp residue with a free vicinal hydroxyl group
-
-
?
acetylglucuronoxylan + H2O
glucuronoxylan + acetate
-
the enzyme deacetylates singly and doubly acetylated xylopyranosyl residues in the polymer to such an extent that it results in the polymer precipitation
-
-
?
acetylxylan + H2O
xylan + acetate
-
60°C, pH 6, acetyl xylan esterase (acetate-releasing) activity on birchwood xylan
-
-
?
bovine submaxillary mucin + H2O
deacetylated bovine submaxillary mucin + acetate
-
-
-
?
geraniol acetate + H2O
geraniol + acetate
-
enzyme plays a role in geraniol production and improving palmarosa oil quality during florescence development
-
?
isoamyl acetate + H2O
isoamyl alcohol + acetate
-
25°C, 1 h, pH 7, 7 mM MgCl2, cell extracts
-
-
?
kojic acid + vinyl acetate
7-O-acetyl-kojic acid + vinyl alcohol
-
yield is 30.9%
-
-
?
methyl 2,3,4-O-triacetyl-beta-D-xylopyranoside + 3 H2O
methyl beta-D-xylopyranoside + 3 acetate
-
all four acetylated derivatives of methyl beta-xylopyranoside are completely deacetylated after 24 h
-
-
?
methyl 2,3,4-O-triacetyl-beta-D-xylopyranoside + H2O
methyl 3,4-O-diacetyl-beta-D-xylopyranoside + acetate
-
-
-
-
?
methyl 2,3-O-diacetyl-beta-D-xylopyranoside + 2 H2O
methyl beta-D-xylopyranoside + 2 acetate
-
all four acetylated derivatives of methyl beta-xylopyranoside are completely deacetylated after 24 h
-
-
?
methyl 2,3-O-diacetyl-beta-D-xylopyranoside + H2O
methyl 2-O-acetyl-beta-D-xylopyranoside + acetate
-
-
-
-
?
methyl 2,4-O-diacetyl-beta-D-xylopyranoside + 2 H2O
methyl beta-D-xylopyranoside + 2 acetate
-
all four acetylated derivatives of methyl beta-xylopyranoside are completely deacetylated after 24 h
-
-
?
methyl 2-O-acetyl-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranoside + H2O
methyl xylotriose + acetate
-
-
-
-
?
methyl 3,4-O-diacetyl-beta-D-xylopyranoside + 2 H2O
methyl beta-D-xylopyranoside + 2 acetate
-
all four acetylated derivatives of methyl beta-xylopyranoside are completely deacetylated after 24 h
-
-
?
methyl 3-O-acetyl-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranoside + H2O
methyl xylotriose + acetate
-
-
-
-
?
methyl 4-O-acetyl-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranoside + H2O
methyl xylotriose + acetate
-
-
-
-
?
methyl beta-D-glucopyranoside + vinyl acetate
methyl 3-O-acetyl-beta-D-glucopyranoside + vinyl alcohol
-
yield is 56.4%
-
-
?
methyl-beta-D-xylopyranoside + vinylacetate
methyl-beta-D-xylopyranoside monoacetate + vinyl alcohol
transacetylation of methylxyloside in aqueous solution (40°C, pH 6, 25 mM methyl-beta-D-xylopyranoside)
analysis by thin-layer chromatography
-
?
O-acetyl-4-O-methyl-D-glucurono-D-xylan + H2O
4-O-methyl-D-glucurono-D-xylan + acetic acid
penta-O-acetyl-alpha-D-glucose + H2O
?
-
complete and regioselective removal of primary acetyl-group in 6-position
-
-
?
racemic 1-phenylethyl acetate + H2O
(1R)-phenylethanol + (1S)-phenylethanol + acetate
-
-
-
-
?
triacetin + H2O
?
-
EST1, pH 8, 20°C, 0.5% (w/v) Triton X-100, 0.125% (w/v) Arabic gum
titration assay
-
?
[(4S)-2,2-dimethyl-1,3-dioxolan-4-yl]methyl acetate + H2O
[(4R)-2,2-dimethyl-1,3-dioxolan-4-yl]methanol + acetate
-
fresh bacterial cells or purified enzyme (EST1 or EST2, 0.01 micromol p-nitrophenol/min), 20°C, pH 8, 5% (v/v) glycerol, 1 mM EDTA, 1 mM dithiothreitol
high enantioselectivity (enantiomeric ratio E: 20 for EST1, E: 15 for whole cells), thin-layer chromatography, enantiomeric composition of reactants controlled by gas chromatography, acetate detection spectrophotometrically through NADH absorbance at 340 nm
-
?
(4R)-hydroxy-3-methyl-2-(2-propynyl)-cyclopent-2-enone acetate + H2O
?
-
highly enantioselective enzyme, enantioselectivity raises by 1.2fold from 16 to 20 when reaction temperature is raised from 30°C to 60°C. Both enantioselectivity and activity are raised by addition of the cationic detergent benzethonium chloride
-
-
?
(4R)-hydroxy-3-methyl-2-(2-propynyl)-cyclopent-2-enone acetate + H2O
?
-
highly enantioselective enzyme, enantioselectivity raises by 1.2fold from 16 to 20 when reaction temperature is raised from 30°C to 60°C. Both enantioselectivity and activity are raised by addition of the cationic detergent benzethonium chloride
-
-
?
(acetyloxy)(phenyl)acetic acid + H2O
(S)-mandelic acid + (2R)-(acetyloxy)(phenyl)ethanoic acid