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(4R)-hydroxy-3-methyl-2-(2-propynyl)-cyclopent-2-enone acetate + H2O
?
(acetyloxy)(phenyl)acetic acid + H2O
(S)-mandelic acid + (2R)-(acetyloxy)(phenyl)ethanoic acid
(R)-1,2-O-isopropylidene glycol acetate + H2O
(R)-1,2-O-isopropylidene glycol + acetate
-
-
-
-
?
(R,S)-1,2-O-isopropylidene glycol butyrate + H2O
(R)-1,2-O-isopropylidene glycol + butyrate
-
fresh bacterial cells or purified EST1 (0.01 micromol p-nitrophenol/min), 28ưC, pH 8, 5% (v/v) glycerol, 1 mM EDTA, 1 mM dithiothreitol
low enantioselectivity (enantiomeric ratio E: 2.5 for EST1, E: 2.5 for whole cells)
-
?
(R,S)-1,2-O-isopropylidene glycol caproate + H2O
(R)-1,2-O-isopropylidene glycol + caproate
-
fresh bacterial cells or purified EST1 (0.01 micromol p-nitrophenol/min), 28ưC, pH 8, 5% (v/v) glycerol, 1 mM EDTA, 1 mM dithiothreitol
no enantioselectivity by EST1 (enantiomeric ratio E: 1) compared to E: 2.8 for whole cells
-
?
1-naphthol acetate + H2O
1-naphthol + acetate
1-naphthyl acetate + H2O
1-naphthol + acetate
1-naphthyl butyrate + H2O
1-naphthol + butyrate
36% activity compared to 1-naphthyl acetate
-
-
?
1-phenyl-1-(trifluoromethyl)prop-2-yn-1-yl acetate + H2O
1-phenyl-1-(trifluoromethyl)prop-2-yn-1-ol + acetate
-
determination of enantiopreference, wild-type R enantioselectivity is 42
spectrophotometric assay coupling released acetate to NADH generation
-
?
13-(6,6'-diacetyl)sophorosyloxydocosanoic acid + H2O
?
Candida bororiensis
-
-
-
-
?
2-benzylcyclohex-1-en-1-yl acetate + H2O
2-benzylcyclohexanone + acetate
2-ethylcyclohex-1-en-1-yl acetate + H2O
2-ethylcyclohexanone + acetate
-
isoenzyme Est I, 99% conversion of substrate with 99% S-configuration of product
-
-
?
2-isopropylcyclohex-1-en-1-yl acetate + H2O
2-isopropylcyclohexanone + acetate
-
isoenzyme Est I, 10% conversion of substrate with R-configuration of product
-
-
?
2-methylcyclohex-1-en-1-yl acetate + H2O
(S)-2-methylcyclohexanone + acetate
-
isoenzyme Est I, 99% conversion of substrate with 99% S-configuration of product
-
-
?
2-n-pentylcyclohex-1-en-1-yl acetate + H2O
2-n-pentylcyclohexanone + acetate
2-n-propylcyclohex-1-en-1-yl acetate + H2O
2-n-propylcyclohexanone + acetate
-
isoenzyme Est I, 99% conversion of substrate with 99% R-configuration of product
-
-
?
2-naphthol acetate + H2O
2-naphthol + acetate
2-naphthyl acetate + H2O
2-naphthol + acetate
2-O-acetyl 4-nitrophenyl beta-D-xylopyranoside + H2O
?
2-O-acetyl-4-nitrophenyl beta-D-xylopyranoside + H2O
?
-
-
-
-
?
2-t-butylcyclohex-1-en-1-yl acetate + H2O
2-t-butylcyclohexanone + acetate
3,6-diacetylmorphine + H2O
morphine + acetate
3-O-acetyl 4-nitrophenyl beta-D-xylopyranoside + H2O
?
3-O-acetyl-4-nitrophenyl beta-D-xylopyranoside + H2O
?
-
-
-
-
?
3-O-methyl-D-glucopyranoside + vinyl acetate
6-O-acetyl-3-O-methyl-D-glucopyranoside + vinyl alcohol
4-acetoxybenzoic acid + H2O
4-hydroxybenzoate + acetate
-
-
-
-
?
4-methylumbelliferone + H2O
?
-
-
-
-
?
4-methylumbelliferyl acetate + H2O
4-methylumbelliferone + acetate
-
-
-
?
4-methylumbelliferyl acetate + H2O
?
4-nitrophenyl 2-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl-beta-D-xylopyranoside + acetate
4-nitrophenyl 3-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl-beta-D-xylopyranoside + acetate
-
-
-
-
?
4-nitrophenyl 4-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl-beta-D-xylopyranoside + acetate
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
4-nitrophenyl beta-D-glucopyranoside + vinyl acetate
4-nitrophenyl 3-O-acetyl-beta-D-glucopyranoside + vinyl alcohol
-
yield is 70.2%
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenyl D-xylopyranose
4 mM substrate, position-specificity, hydrolysis activity is specific for 3-, and 4-O-acetyl 4-nitrophenyl-beta-D-xylopyranoside, 2-O-acetyl 4-nitrophenyl-beta-D-xylopyranoside is a bad substrate
beta-xylosidase-coupled assay
-
?
4-nitrophenyl butyrate + H2O
4-nitrophenol + butyrate
4-nitrophenyl formate + H2O
4-nitrophenol + formate
5 min, 30ưC, pH 5.8
-
-
?
4-nitrophenyl hexanoate + H2O
4-nitrophenol + hexanoate
-
-
-
-
?
4-nitrophenyl propionate + H2O
4-nitrophenol + propionate
4-nitrophenyl-2-O-acetyl-alpha-L-arabinofuranoside + H2O
4-nitrophenyl-alpha-L-arabinofuranoside + acetate
-
-
-
-
?
4-nitrophenyl-2-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl-beta-D-xylopyranoside + acetate
-
preferred substrate
-
-
?
4-nitrophenyl-3-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl-beta-D-xylopyranoside + acetate
-
-
-
-
?
4-nitrophenyl-4-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl-beta-D-xylopyranoside + acetate
-
-
-
-
?
4-nitrophenyl-5-O-acetyl-alpha-L-arabinofuranoside + H2O
4-nitrophenyl-alpha-L-arabinofuranoside + acetate
-
-
-
-
?
4-nitrophenyl-butanoate + H2O
4-nitrophenol + butanoate
-
-
-
-
?
4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside + H2O
?
4-O-acetyl-4-nitrophenyl beta-D-xylopyranoside + H2O
?
-
-
-
-
?
6-acetylmorphine + H2O
morphine + acetate
6-O-acetyl-D-mannopyranose + H2O
?
-
-
-
-
?
6-O-acetyl-D-mannopyranoside + H2O
?
-
-
-
-
?
7-aminocephalosporanic acid + H2O
?
-
-
-
-
?
7-aminocephalosporanic acid + H2O
deacetylated 7-aminocephalosporanic acid + acetate
-
-
-
-
?
9-O-acetyl sialic acid + H2O
sialic acid + acetate
-
-
-
?
acetyl alginate + H2O
alginate + acetate
-
-
-
?
acetyl xylan + H2O
xylan + acetate
-
from beech wood or oat spelt
-
?
acetylated hemicellulose + H2O
? + acetate
-
the best substrate of the enzyme is 3-O-acetylated Xylp residue followed by 4-O-acetylated Xylp residue with a free vicinal hydroxyl group
-
-
?
acetylglucuronoxylan + H2O
glucuronoxylan + acetate
-
the enzyme deacetylates singly and doubly acetylated xylopyranosyl residues in the polymer to such an extent that it results in the polymer precipitation
-
-
?
acetylhemicellulose + H2O
hemicellulose + acetate
-
-
-
-
?
acetylxylan + H2O
xylan + acetate
-
60ưC, pH 6, acetyl xylan esterase (acetate-releasing) activity on birchwood xylan
-
-
?
alpha,alpha-trehalose + vinyl acetate
? + vinyl alcohol
-
-
-
-
?
alpha-naphthyl acetate + H2O
alpha-naphthol + acetate
an acetic ester + H2O
an alcohol + acetate
-
-
-
-
?
apigenin 7-O-(6-O-malonylglucoside) + H2O
?
-
-
-
-
?
arabinogalactan + H2O
?
-
-
-
-
?
astragaloside I + H2O
astragaloside IV + ?
astragaloside II + H2O
astragaloside IV + ?
beechwood xylan + H2O
?
-
-
-
-
?
beta-naphthyl acetate + H2O
beta-naphthol + acetate
birchwood xylan + H2O
?
-
-
-
-
?
bovine submaxillary mucin + H2O
deacetylated bovine submaxillary mucin + acetate
-
-
-
?
cellobiose + vinyl acetate
? + vinyl alcohol
cellobiose octaacetate + H2O
?
-
low activity
-
?
cellulose acetate + H2O
cellulose + acetate
cephalosporin C + H2O
deacetylated cephalosporin C + acetate
-
-
-
-
?
cephalosporin C + H2O
deacetylcephalosporin C + acetate
-
-
-
-
?
chlorogenic acid + H2O
?
-
-
-
-
?
D-galactose + vinyl acetate
? + vinyl alcohol
D-glucopyranosyl beta-(1,3)-xylopyranoside + vinyl acetate
? + vinyl alcohol
D-glucopyranosyl beta-(1,4)-xylopyranoside + vinyl acetate
? + vinyl alcohol
D-glucose + vinyl acetate
6-O-acetyl-D-glucopyranose + vinyl alcohol
D-glucose penta-acetate + H2O
?
D-mannose + vinyl acetate
6-O-acetyl-D-mannopyranose + vinyl alcohol
D-xylopyranosyl beta-(1,4)-D-glucopyranoside + vinyl acetate
? + vinyl alcohol
diacetin + H2O
?
-
-
-
-
?
ethyl acetate + H2O
ethanol + acetate
geraniol acetate + H2O
geraniol + acetate
-
enzyme plays a role in geraniol production and improving palmarosa oil quality during florescence development
-
?
geranyl acetate + H2O
geraniol + acetate
-
-
-
?
glucose penta-acetate + H2O
?
-
high activity
-
-
?
glucose pentaacetate + H2O
?
glycerol triacetate + H2O
?
isoamyl acetate + H2O
isoamyl alcohol + acetate
-
25ưC, 1 h, pH 7, 7 mM MgCl2, cell extracts
-
-
?
isoastragaloside I + H2O
astragaloside IV + ?
isoastragaloside II + H2O
astragaloside IV + ?
kojic acid + vinyl acetate
7-O-acetyl-kojic acid + vinyl alcohol
-
yield is 30.9%
-
-
?
malonylated isorhamnetin 3-O-glucoside + H2O
?
-
-
-
-
?
methyl 2,3,4-O-triacetyl-beta-D-xylopyranoside + H2O
methyl 3,4-O-diacetyl-beta-D-xylopyranoside + acetate
-
-
-
-
?
methyl 2,3,4-O-triacetyl-beta-D-xylopyranoside + H2O
methyl beta-D-xylopyranoside + acetate
-
all four acetylated derivatives of methyl beta-xylopyranoside are completely deacetylated after 24 h
-
-
?
methyl 2,3-O-diacetyl-beta-D-xylopyranoside + H2O
?
methyl 2,3-O-diacetyl-beta-D-xylopyranoside + H2O
methyl 2-O-acetyl-beta-D-xylopyranoside + acetate
-
-
-
-
?
methyl 2,3-O-diacetyl-beta-D-xylopyranoside + H2O
methyl beta-D-xylopyranoside + acetate
-
all four acetylated derivatives of methyl beta-xylopyranoside are completely deacetylated after 24 h
-
-
?
methyl 2,4-O-diacetyl-beta-D-xylopyranoside + 2 H2O
methyl beta-D-xylopyranoside + 2 acetate
-
all four acetylated derivatives of methyl beta-xylopyranoside are completely deacetylated after 24 h
-
-
?
methyl 2,4-O-diacetyl-beta-D-xylopyranoside + H2O
?
methyl 2-O-acetyl-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranoside + H2O
methyl xylotriose + acetate
-
-
-
-
?
methyl 2-O-methyl-beta-D-glucopyranoside + vinyl acetate
? + vinyl alcohol
methyl 3,4-O-diacetyl-beta-D-xylopyranoside + 2 H2O
methyl beta-D-xylopyranoside + 2 acetate
-
all four acetylated derivatives of methyl beta-xylopyranoside are completely deacetylated after 24 h
-
-
?
methyl 3,4-O-diacetyl-beta-D-xylopyranoside + H2O
?
methyl 3-O-acetyl-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranoside + H2O
methyl xylotriose + acetate
-
-
-
-
?
methyl 4-O-acetyl-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranoside + H2O
methyl xylotriose + acetate
-
-
-
-
?
methyl 4-O-methyl-alpha-D-glucopyranoside + vinyl acetate
? + vinyl alcohol
methyl 6-O-acetyl-beta-D-glucopyranoside + H2O
?
methyl acetate + H2O
methanol + acetate
methyl alpha-D-glucopyranoside + vinyl acetate
? + vinyl alcohol
methyl beta-D-glucopyranoside + vinyl acetate
? + vinyl alcohol
methyl beta-D-glucopyranoside + vinyl acetate
methyl 3-O-acetyl-beta-D-glucopyranoside + vinyl alcohol
-
yield is 56.4%
-
-
?
methyl-beta-D-xylopyranoside + vinylacetate
methyl-beta-D-xylopyranoside monoacetate + vinyl alcohol
transacetylation of methylxyloside in aqueous solution (40ưC, pH 6, 25 mM methyl-beta-D-xylopyranoside)
analysis by thin-layer chromatography
-
?
monoacetin + H2O
glycerol + acetate
-
-
-
-
?
naphthyl acetate + H2O
naphthol + acetate
-
-
-
-
?
naphthyl butyrate + H2O
naphthol + butyrate
-
-
-
-
?
naphthyl propionate + H2O
naphthol + propionate
-
-
-
-
?
O-acetyl-1,4-beta-xylooligosaccharide + H2O
1,4-beta-xylooligosaccharide + acetic acid
O-acetyl-4-O-methyl-D-glucurono-D-xylan + H2O
4-O-methyl-D-glucurono-D-xylan + acetic acid
O-acetyl-galactoglucomannan + H2O
?
-
-
-
-
?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
p-nitrophenyl butyrate + H2O
p-nitrophenol + butyrate
-
-
-
-
?
p-nitrophenyl decanoate + H2O
p-nitrophenol + decanoate
-
-
-
-
?
p-nitrophenyl hexanoate + H2O
p-nitrophenol + hexanoate
p-nitrophenyl laurate + H2O
p-nitrophenol + laurate
p-nitrophenyl propionate + H2O
p-nitrophenol + propionate
penta-O-acetyl-alpha-D-glucose + H2O
?
-
complete and regioselective removal of primary acetyl-group in 6-position
-
-
?
phenyl acetate + H2O
phenol + acetate
poly(vinylacetate) + H2O
? + acetate
-
-
-
-
?
polygalacturonan + H2O
?
-
-
-
-
?
potato pectin + H2O
? + acetate
-
-
-
-
?
racemic 1-phenylethyl acetate + H2O
(1R)-phenylethanol + (1S)-phenylethanol + acetate
-
-
-
-
?
sugar beet pectin + H2O
? + acetate
-
highest activity
-
-
?
tert-butyl acetate + H2O
2-methylpropane + acetate
-
-
-
?
tobacco hemicellulose + H2O
?
-
lowest activity
-
-
?
triacetin + H2O
?
-
EST1, pH 8, 20ưC, 0.5% (w/v) Triton X-100, 0.125% (w/v) Arabic gum
titration assay
-
?
triacetine + H2O
?
-
-
-
-
?
tripropionin + H2O
?
-
-
-
-
?
xylobiose + vinyl acetate
? + vinyl alcohol
xylose tetraacetate + H2O
?
[(4S)-2,2-dimethyl-1,3-dioxolan-4-yl]methyl acetate + H2O
[(4R)-2,2-dimethyl-1,3-dioxolan-4-yl]methanol + acetate
-
fresh bacterial cells or purified enzyme (EST1 or EST2, 0.01 micromol p-nitrophenol/min), 20ưC, pH 8, 5% (v/v) glycerol, 1 mM EDTA, 1 mM dithiothreitol
high enantioselectivity (enantiomeric ratio E: 20 for EST1, E: 15 for whole cells), thin-layer chromatography, enantiomeric composition of reactants controlled by gas chromatography, acetate detection spectrophotometrically through NADH absorbance at 340 nm
-
?
additional information
?
-
(4R)-hydroxy-3-methyl-2-(2-propynyl)-cyclopent-2-enone acetate + H2O

?
-
highly enantioselective enzyme, enantioselectivity raises by 1.2fold from 16 to 20 when reaction temperature is raised from 30ưC to 60ưC. Both enantioselectivity and activity are raised by addition of the cationic detergent benzethonium chloride
-
-
?
(4R)-hydroxy-3-methyl-2-(2-propynyl)-cyclopent-2-enone acetate + H2O
?
-
highly enantioselective enzyme, enantioselectivity raises by 1.2fold from 16 to 20 when reaction temperature is raised from 30ưC to 60ưC. Both enantioselectivity and activity are raised by addition of the cationic detergent benzethonium chloride
-
-
?
(acetyloxy)(phenyl)acetic acid + H2O

(S)-mandelic acid + (2R)-(acetyloxy)(phenyl)ethanoic acid
-
-
-
?
(acetyloxy)(phenyl)acetic acid + H2O
(S)-mandelic acid + (2R)-(acetyloxy)(phenyl)ethanoic acid
-
-
-
?
1-naphthol acetate + H2O

1-naphthol + acetate
-
-
-
-
?
1-naphthol acetate + H2O
1-naphthol + acetate
-
-
-
-
?
1-naphthol acetate + H2O
1-naphthol + acetate
-
-
-
-
?
1-naphthol acetate + H2O
1-naphthol + acetate
-
-
-
-
?
1-naphthyl acetate + H2O

1-naphthol + acetate
-
-
product determination by NMR and mass spectroscopy
-
?
1-naphthyl acetate + H2O
1-naphthol + acetate
-
-
-
?
1-naphthyl acetate + H2O
1-naphthol + acetate
-
-
-
?
1-naphthyl acetate + H2O
1-naphthol + acetate
highest activity (100%)
-
-
?
1-naphthyl acetate + H2O
1-naphthol + acetate
-
-
-
-
?
1-naphthyl acetate + H2O
1-naphthol + acetate
-
-
-
-
?
2-benzylcyclohex-1-en-1-yl acetate + H2O

2-benzylcyclohexanone + acetate
-
isoenzyme Est I, 99% conversion of substrate with 99% S-configuration of product
-
-
?
2-benzylcyclohex-1-en-1-yl acetate + H2O
2-benzylcyclohexanone + acetate
-
isoenzyme Est II, 11% conversion of substrate with R-configuration of product
-
-
?
2-n-pentylcyclohex-1-en-1-yl acetate + H2O

2-n-pentylcyclohexanone + acetate
-
isoenzyme Est I, 20% conversion of substrate with R-configuration of product
-
-
?
2-n-pentylcyclohex-1-en-1-yl acetate + H2O
2-n-pentylcyclohexanone + acetate
-
isoenzyme Est II, 16% conversion of substrate with S-configuration of product
-
-
?
2-naphthol acetate + H2O

2-naphthol + acetate
-
-
-
-
?
2-naphthol acetate + H2O
2-naphthol + acetate
-
-
-
-
?
2-naphthyl acetate + H2O

2-naphthol + acetate
-
-
-
-
?
2-naphthyl acetate + H2O
2-naphthol + acetate
about 40% activity compared to 1-naphthyl acetate
-
-
?
2-naphthyl acetate + H2O
2-naphthol + acetate
-
-
-
-
?
2-O-acetyl 4-nitrophenyl beta-D-xylopyranoside + H2O

?
-
-
-
-
?
2-O-acetyl 4-nitrophenyl beta-D-xylopyranoside + H2O
?
-
-
-
-
?
2-t-butylcyclohex-1-en-1-yl acetate + H2O

2-t-butylcyclohexanone + acetate
-
isoenzyme Est I, 21% conversion of substrate with S-configuration of product
-
-
?
2-t-butylcyclohex-1-en-1-yl acetate + H2O
2-t-butylcyclohexanone + acetate
-
isoenzyme Est II, 20% conversion of substrate with R-configuration of product
-
-
?
3,6-diacetylmorphine + H2O

morphine + acetate
-
i.e. heroin, rapid spontaneous hydrolysis of the instable 3-acetyl group
-
?
3,6-diacetylmorphine + H2O
morphine + acetate
-
i.e. heroin, rapid spontaneous hydrolysis of the instable 3-acetyl group
-
?
3-O-acetyl 4-nitrophenyl beta-D-xylopyranoside + H2O

?
-
-
-
-
?
3-O-acetyl 4-nitrophenyl beta-D-xylopyranoside + H2O
?
-
-
-
-
?
3-O-methyl-D-glucopyranoside + vinyl acetate

6-O-acetyl-3-O-methyl-D-glucopyranoside + vinyl alcohol
-
-
-
-
?
3-O-methyl-D-glucopyranoside + vinyl acetate
6-O-acetyl-3-O-methyl-D-glucopyranoside + vinyl alcohol
-
-
-
-
?
4-methylumbelliferyl acetate + H2O

?
-
-
-
-
?
4-methylumbelliferyl acetate + H2O
?
-
-
-
-
?
4-methylumbelliferyl acetate + H2O
?
-
-
-
-
?
4-nitrophenyl 2-O-acetyl-beta-D-xylopyranoside + H2O

4-nitrophenyl-beta-D-xylopyranoside + acetate
-
-
-
-
?
4-nitrophenyl 2-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl-beta-D-xylopyranoside + acetate
-
-
-
-
?
4-nitrophenyl acetate + H2O

4-nitrophenol + acetate
-
serine esterase activity
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
determination of specific activity
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
product determination by NMR and mass spectroscopy
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
highest activity
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
highest activity
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
highest activity
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
5 min, 30ưC, pH 5.8, for kinetic analyses: 0.05-5 mM substrate in 100 mM sodium phosphate buffer
analysis by spectrophotometry at 410 nm
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
-
?
4-nitrophenyl butyrate + H2O

4-nitrophenol + butyrate
-
-
-
-
?
4-nitrophenyl butyrate + H2O
4-nitrophenol + butyrate
-
-
-
?
4-nitrophenyl butyrate + H2O
4-nitrophenol + butyrate
-
lowest activity
-
-
?
4-nitrophenyl butyrate + H2O
4-nitrophenol + butyrate
-
lowest activity
-
-
?
4-nitrophenyl butyrate + H2O
4-nitrophenol + butyrate
-
-
-
-
?
4-nitrophenyl butyrate + H2O
4-nitrophenol + butyrate
5 min, 30ưC, pH 5.8
-
-
?
4-nitrophenyl propionate + H2O

4-nitrophenol + propionate
-
-
-
-
?
4-nitrophenyl propionate + H2O
4-nitrophenol + propionate
-
-
-
-
?
4-nitrophenyl propionate + H2O
4-nitrophenol + propionate
-
-
-
-
?
4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside + H2O

?
-
-
-
-
?
4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside + H2O
?
-
-
-
-
?
6-acetylmorphine + H2O

morphine + acetate
-
substrate specificity
-
?
6-acetylmorphine + H2O
morphine + acetate
-
substrate specificity
-
?
acetylxylan + H2O

?
-
-
-
-
?
acetylxylan + H2O
?
-
-
-
-
?
acetylxylan + H2O
?
-
-
-
-
?
acetylxylan + H2O
?
-
-
-
-
?
acetylxylan + H2O
?
-
-
-
-
?
acetylxylan + H2O
?
-
-
-
?
acetylxylan + H2O
?
-
-
-
?
acetylxylan + H2O
?
-
-
-
-
?
acetylxylan + H2O
?
-
-
-
-
?
acetylxylan + H2O
?
-
-
-
-
?
acetylxylan + H2O
?
-
-
-
-
?
acetylxylan + H2O
?
-
-
-
-
?
acetylxylan + H2O
?
-
-
-
-
?
alpha-naphthyl acetate + H2O

alpha-naphthol + acetate
-
-
-
-
?
alpha-naphthyl acetate + H2O
alpha-naphthol + acetate
-
-
-
-
?
alpha-naphthyl acetate + H2O
alpha-naphthol + acetate
-
EST1, non-denaturating PAGE, pH7.4
zymogram analyses using Fast Blue PR salt
-
?
alpha-naphthyl acetate + H2O
alpha-naphthol + acetate
-
EST1, non-denaturating PAGE, pH7.4
zymogram analyses using Fast Blue PR salt
-
?
alpha-naphthyl acetate + H2O
alpha-naphthol + acetate
-
15 min, 50ưC, pH 6.5
-
-
?
alpha-naphthyl acetate + H2O
alpha-naphthol + acetate
-
-
-
-
?
alpha-naphthyl acetate + H2O
alpha-naphthol + acetate
-
-
-
-
?
astragaloside I + H2O

astragaloside IV + ?
-
-
i.e. 3-O-beta-D-xylopyranosyl-6-O-beta-D-glucopyranosyl-cycloastragenol
-
?
astragaloside I + H2O
astragaloside IV + ?
-
-
i.e. 3-O-beta-D-xylopyranosyl-6-O-beta-D-glucopyranosyl-cycloastragenol
-
?
astragaloside II + H2O

astragaloside IV + ?
-
-
i.e. 3-O-beta-D-xylopyranosyl-6-O-beta-D-glucopyranosyl-cycloastragenol
-
?
astragaloside II + H2O
astragaloside IV + ?
-
-
i.e. 3-O-beta-D-xylopyranosyl-6-O-beta-D-glucopyranosyl-cycloastragenol
-
?
beta-naphthyl acetate + H2O

beta-naphthol + acetate
-
-
-
?
beta-naphthyl acetate + H2O
beta-naphthol + acetate
-
sucrose density gradient cell fractions from Hep-G2 and RINm5F cells, pH 8
SDS-PAGE and in gel-zymography
-
?
cellobiose + vinyl acetate

? + vinyl alcohol
-
-
-
-
?
cellobiose + vinyl acetate
? + vinyl alcohol
-
-
-
-
?
cellulose acetate + H2O

cellulose + acetate
-
also in form of acetylated starch, water-soluble and water-insoluble substrate, specific for acetyl substituents at the C2- and C3-positions, C6-acetyl is not hydrolyzed
-
?
cellulose acetate + H2O
cellulose + acetate
-
-
-
-
?
cellulose acetate + H2O
cellulose + acetate
-
-
-
?
D-galactose + vinyl acetate

? + vinyl alcohol
-
isozyme CE2B shows 22% transacetylation conversion, isozyme CE2A shows 48% transacetylation conversion
-
-
?
D-galactose + vinyl acetate
? + vinyl alcohol
-
isozyme CE2B shows 89% transacetylation conversion
-
-
?
D-glucopyranosyl beta-(1,3)-xylopyranoside + vinyl acetate

? + vinyl alcohol
-
-
-
-
?
D-glucopyranosyl beta-(1,3)-xylopyranoside + vinyl acetate
? + vinyl alcohol
-
-
-
-
?
D-glucopyranosyl beta-(1,4)-xylopyranoside + vinyl acetate

? + vinyl alcohol
-
-
-
-
?
D-glucopyranosyl beta-(1,4)-xylopyranoside + vinyl acetate
? + vinyl alcohol
-
-
-
-
?
D-glucose + vinyl acetate

6-O-acetyl-D-glucopyranose + vinyl alcohol
-
isozyme CE2B shows 31% transacetylation conversion, isozyme CE2A shows 25% transacetylation conversion
-
-
?
D-glucose + vinyl acetate
6-O-acetyl-D-glucopyranose + vinyl alcohol
-
isozyme CE2B shows 86% transacetylation conversion
-
-
?
D-glucose penta-acetate + H2O

?
-
-
-
-
?
D-glucose penta-acetate + H2O
?
-
-
-
?
D-mannose + vinyl acetate

6-O-acetyl-D-mannopyranose + vinyl alcohol
-
isozyme CE2B shows 46% transacetylation conversion, isozyme CE2A shows 26% transacetylation conversion
-
-
?
D-mannose + vinyl acetate
6-O-acetyl-D-mannopyranose + vinyl alcohol
-
isozyme CE2B shows 71% transacetylation conversion
-
-
?
D-xylopyranosyl beta-(1,4)-D-glucopyranoside + vinyl acetate

? + vinyl alcohol
-
-
-
-
?
D-xylopyranosyl beta-(1,4)-D-glucopyranoside + vinyl acetate
? + vinyl alcohol
-
-
-
-
?
ethyl acetate + H2O

ethanol + acetate
-
-
-
-
?
ethyl acetate + H2O
ethanol + acetate
-
-
-
-
?
ethyl acetate + H2O
ethanol + acetate
-
25ưC, 1 h, pH 7, 7 mM MgCl2, cell extracts
-
-
?
glucose pentaacetate + H2O

?
-
good substrate
-
?
glucose pentaacetate + H2O
?
-
-
-
-
?
glucose pentaacetate + H2O
?
-
-
-
-
?
glycerol triacetate + H2O

?
-
-
-
-
?
glycerol triacetate + H2O
?
-
-
-
-
?
isoastragaloside I + H2O

astragaloside IV + ?
-
-
i.e. 3-O-beta-D-xylopyranosyl-6-O-beta-D-glucopyranosyl-cycloastragenol
-
?
isoastragaloside I + H2O
astragaloside IV + ?
-
-
i.e. 3-O-beta-D-xylopyranosyl-6-O-beta-D-glucopyranosyl-cycloastragenol
-
?
isoastragaloside II + H2O

astragaloside IV + ?
-
-
i.e. 3-O-beta-D-xylopyranosyl-6-O-beta-D-glucopyranosyl-cycloastragenol
-
?
isoastragaloside II + H2O
astragaloside IV + ?
-
-
i.e. 3-O-beta-D-xylopyranosyl-6-O-beta-D-glucopyranosyl-cycloastragenol
-
?
methyl 2,3-O-diacetyl-beta-D-xylopyranoside + H2O

?
-
-
-
-
?
methyl 2,3-O-diacetyl-beta-D-xylopyranoside + H2O
?
-
-
-
-
?
methyl 2,4-O-diacetyl-beta-D-xylopyranoside + H2O

?
-
-
-
-
?
methyl 2,4-O-diacetyl-beta-D-xylopyranoside + H2O
?
-
-
-
-
?
methyl 2-O-methyl-beta-D-glucopyranoside + vinyl acetate

? + vinyl alcohol
-
-
-
-
?
methyl 2-O-methyl-beta-D-glucopyranoside + vinyl acetate
? + vinyl alcohol
-
-
-
-
?
methyl 3,4-O-diacetyl-beta-D-xylopyranoside + H2O

?
-
-
-
-
?
methyl 3,4-O-diacetyl-beta-D-xylopyranoside + H2O
?
-
-
-
-
?
methyl 4-O-methyl-alpha-D-glucopyranoside + vinyl acetate

? + vinyl alcohol
-
-
-
-
?
methyl 4-O-methyl-alpha-D-glucopyranoside + vinyl acetate
? + vinyl alcohol
-
-
-
-
?
methyl 6-O-acetyl-beta-D-glucopyranoside + H2O

?
-
-
-
-
?
methyl 6-O-acetyl-beta-D-glucopyranoside + H2O
?
-
-
-
-
?
methyl acetate + H2O

methanol + acetate
-
-
-
-
?
methyl acetate + H2O
methanol + acetate
-
-
-
-
?
methyl alpha-D-glucopyranoside + vinyl acetate

? + vinyl alcohol
-
-
-
-
?
methyl alpha-D-glucopyranoside + vinyl acetate
? + vinyl alcohol
-
-
-
-
?
methyl beta-D-glucopyranoside + vinyl acetate

? + vinyl alcohol
-
-
-
-
?
methyl beta-D-glucopyranoside + vinyl acetate
? + vinyl alcohol
-
-
-
-
?
O-acetyl-1,4-beta-xylooligosaccharide + H2O

1,4-beta-xylooligosaccharide + acetic acid
-
-
-
ir
O-acetyl-1,4-beta-xylooligosaccharide + H2O
1,4-beta-xylooligosaccharide + acetic acid
-
-
-
-
ir
O-acetyl-4-O-methyl-D-glucurono-D-xylan + H2O

4-O-methyl-D-glucurono-D-xylan + acetic acid
-
-
-
-
?
O-acetyl-4-O-methyl-D-glucurono-D-xylan + H2O
4-O-methyl-D-glucurono-D-xylan + acetic acid
-
-
-
ir
p-nitrophenyl acetate + H2O

p-nitrophenol + acetate
-
-
-
-
?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
-
-
-
-
?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
-
-
-
-
?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
-
-
-
-
?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
-
-
-
-
?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
Candida bororiensis
-
-
-
-
?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
-
EST1, pH 7.4, 20ưC, 0.5% (w/v) Triton X-100, 0.125% (w/v) Arabic gum
spectrophotometry, 400 nm
-
?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
-
-
-
-
?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
-
-
-
-
?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
-
-
-
-
?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
-
-
-
-
?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
-
10 min, 50ưC, pH 6.5
-
-
?
p-nitrophenyl hexanoate + H2O

p-nitrophenol + hexanoate
-
-
-
-
?
p-nitrophenyl hexanoate + H2O
p-nitrophenol + hexanoate
-
-
-
-
?
p-nitrophenyl laurate + H2O

p-nitrophenol + laurate
-
-
-
-
?
p-nitrophenyl laurate + H2O
p-nitrophenol + laurate
-
-
-
-
?
p-nitrophenyl propionate + H2O

p-nitrophenol + propionate
-
-
-
-
?
p-nitrophenyl propionate + H2O
p-nitrophenol + propionate
-
-
-
-
?
phenyl acetate + H2O

phenol + acetate
-
sucrose density gradient cell fractions from Hep-G2 and RINm5F cells, pH 8, 3 min, 1 mM CaCl2 or 5 mM EDTA
spectrophotometry, absorbance at 270 nm
-
?
phenyl acetate + H2O
phenol + acetate
-
-
-
?
phenyl acetate + H2O
phenol + acetate
-
-
-
?
tributyrin + H2O

?
-
-
-
?
tributyrin + H2O
?
-
EST1, pH 8, 20ưC, 0.5% (w/v) Triton X-100, 0.125% (w/v) Arabic gum
titration assay
-
?
xanthan + H2O

?
the enzyme removes 57% of all acetyl groups present in xanthan after a 72 h incubation which corresponds to the removal of about 70% of all acetyl groups positioned on the inner mannose unit
-
-
?
xanthan + H2O
?
the enzyme removes 57% of all acetyl groups present in xanthan after a 72 h incubation which corresponds to the removal of about 70% of all acetyl groups positioned on the inner mannose unit
-
-
?
xylobiose + vinyl acetate

? + vinyl alcohol
-
-
-
-
?
xylobiose + vinyl acetate
? + vinyl alcohol
-
-
-
-
?
xylose tetraacetate + H2O

?
-
-
-
-
?
xylose tetraacetate + H2O
?
-
-
-
-
?
additional information

?
-
-
enzyme has receptor-destroying enzyme function
-
?
additional information
?
-
-
substrate specificity and regioselectivity, deacetylation activity of the enzyme is not restricted to polymers with beta-1,4 glycosidic bonds, no activity with cellulose sulfate, the enzyme enhances endoglucanase, but is itself not influenced by endoglucanase
-
?
additional information
?
-
-
the enzyme, feruloyl esterase AoFae, behaves as a non-specific acetyl esterase rather than a feruloyl esterase, with a preference for 2-O-acetyl-beta-D-xylopyranoside, AoFae shows only a very weak activity on feruloyl esters
-
-
?
additional information
?
-
-
1-(4-fluorophenyl)-1-(trifluoromethyl)prop-2-yn-1-yl acetate, 1-(4-chlorophenyl)-1-(trifluoromethyl)prop-2-yn-1-yl acetate, 1-(4-methylphenyl)-1-(trifluoromethyl)prop-2-yn-1-yl acetate, 1-(3-fluorophenyl)-1-(trifluoromethyl)prop-2-yn-1-yl acetate, 1-(3,4-difluorophenyl)-1-(trifluoromethyl)prop-2-yn-1-yl acetate are substrates of double mutant E188W/M193C, conversion with inversed enantioselectivity
-
-
?
additional information
?
-
-
1-phenyl-1-(trifluoromethyl)but-2-yn-1-yl acetate and 1-phenyl-1-(trifluoromethyl)prop-2-en-1-yl acetate are no substrates of neither wild-type nor any mutant
-
-
?
additional information
?
-
-
size of residue at position 188 (from Asp to Trp) determines enantioselectivity and -preference
-
-
?
additional information
?
-
-
no transacetylation occurs with vinyl acetate and D-xylose, xylobiose, methyl 6-O-methyl-alpha-D-glucopyranoside, 6-O-methyl D-glucopyranose, and methyl beta-D-xylopyranoside
-
-
?
additional information
?
-
-
the enzyme shows a strong preference for deacetylation of the 6-position in aldohexoses. Xylose and xylooligosaccharides do not serve as acetyl group acceptors
-
-
?
additional information
?
-
-
removal of acetyl group in 3-position of beta-lactams with 98% conversion and 91-93% product yield
-
-
?
additional information
?
-
-
the enzyme deacetylates cereal products like water soluble polysaccharides such as ragi, wheat, larch wood xylan, and gum karaya, the enzyme cleaves the acetyl groups substituted at O-2/O-3 of the xylan backbone of arabinoxylans and is known to modulate their functional properties
-
-
?
additional information
?
-
-
activated Sepharose CL6B-bound activity, pH 8
-
-
?
additional information
?
-
-
no transacetylation occurs with vinyl acetate and D-xylose, xylobiose, methyl 6-O-methyl-alpha-D-glucopyranoside, 6-O-methyl D-glucopyranose, and methyl beta-D-xylopyranoside
-
-
?
additional information
?
-
-
the enzyme shows a strong preference for deacetylation of the 6-position in aldohexoses. Xylose and xylooligosaccharides do not serve as acetyl group acceptors
-
-
?
additional information
?
-
-
activity of EST1 only on short chain triglyceride substrates
-
-
?
additional information
?
-
-
EST2 shows lower activity and enantioselectivity towards (R,S)-1,2-O-isopropylidene glycol acetate as substrate than EST1
-
-
?
additional information
?
-
-
no activity of EST1 on p-nitrophenyl butyrate, p-nitrophenyl caproate, p-nitrophenyl caprylate, p-nitrophenyl laurate and p-nitrophenyl palmitate
-
-
?
additional information
?
-
-
no activity of EST1 on triacylglyceride esters tricaprylin and triolein
-
-
?
additional information
?
-
-
activity of EST1 only on short chain triglyceride substrates
-
-
?
additional information
?
-
-
EST2 shows lower activity and enantioselectivity towards (R,S)-1,2-O-isopropylidene glycol acetate as substrate than EST1
-
-
?
additional information
?
-
-
no activity of EST1 on p-nitrophenyl butyrate, p-nitrophenyl caproate, p-nitrophenyl caprylate, p-nitrophenyl laurate and p-nitrophenyl palmitate
-
-
?
additional information
?
-
-
no activity of EST1 on triacylglyceride esters tricaprylin and triolein
-
-
?
additional information
?
-
-
the enzyme does not attack the 3-O-acetyl group of xylopyranosyl residues carrying 4-O-methyl-D-glucuronic acid at position 2
-
-
?
additional information
?
-
-
the enzyme does not attack the 3-O-acetyl group of xylopyranosyl residues carrying 4-O-methyl-D-glucuronic acid at position 2
-
-
?
additional information
?
-
-
the enzyme has no activity toward 4-nitrophenyl valerate, 4-nitrophenyl caproate, 4-nitrophenyl palmitate, 4-nitrophenyl benzoate, 4-nitrophenyl phosphate, methyl benzoate, methyl 4-hydroxybenzoate, or methyl cyclohexane-1-carboxylate
-
-
?
additional information
?
-
no activity with 1-naphthyl phosphate, 4-methylumbelliferyl phosphate, linalyl acetate, alpha-terpinyl acetate, glyceryl trioleate, fish oil, or olive oil
-
-
?
additional information
?
-
-
no activity with 1-naphthyl phosphate, 4-methylumbelliferyl phosphate, linalyl acetate, alpha-terpinyl acetate, glyceryl trioleate, fish oil, or olive oil
-
-
?
additional information
?
-
-
no activity on p-nitrophenyl-beta-D-xylopyranoside and p-nitrophenyl-alpha-L-arabinofuranoside and no xylanolytic activity
-
-
?
additional information
?
-
-
no activity with 4-nitrophenyl octanoate. The enzyme does not exhibit de-esterification activity toward an acetylated xylooligosaccharide extracted from hot-water treated eucalyptus
-
-
?
additional information
?
-
-
the enzyme has no activity with acetylated xylans
-
-
?
additional information
?
-
-
no activity is detected on xylan or acetylated xylan or N-acetyl-D-glucosamine
-
-
?
additional information
?
-
new family of microbial esterases with hemicellulase activity for biodegradation of lignocellulose, complementary activity to acetylxylan esterase Axe1
-
-
?
additional information
?
-
-
acetylesterases generally prefer aliphatic substrates involving acetic acid
-
-
?
additional information
?
-
-
isoamyl acetate and ethyl acetate-synthesizing esterase activities in mutant strain 100fold less than respective acetate ester-hydrolysing esterase activities
-
-
?
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0.07
(R)-1,2-O-isopropylidene glycol acetate
-
EST1, after preparative electrophoresis
2.63
1-acetoxynaphthalene
-
pH 7.8, 45ưC
1.54
1-naphthol acetate
-
-
0.06 - 0.97
2-benzylcyclohex-1-en-1-yl acetate
0.11
2-ethylcyclohex-1-en-1-yl acetate
-
isoenzyme Est I, pH 7.0, 35ưC
1.87
2-isopropylcyclohex-1-en-1-yl acetate
-
isoenzyme Est I, pH 7.0, 35ưC
0.09
2-methylcyclohex-1-en-1-yl acetate
-
isoenzyme Est I, pH 7.0, 35ưC
0.3 - 0.72
2-n-pentylcyclohex-1-en-1-yl acetate
0.54
2-n-propylcyclohex-1-en-1-yl acetate
-
isoenzyme Est I, pH 7.0, 35ưC
4.42
2-O-acetyl 4-nitrophenyl beta-D-xylopyranoside
-
isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40ưC
3.6
2-O-acetyl-4-nitrophenyl beta-D-xylopyranoside
-
in 50 mM citrate-phosphate (pH 6) at 70ưC
2.01 - 2.54
2-t-butylcyclohex-1-en-1-yl acetate
1.55
3-O-acetyl 4-nitrophenyl beta-D-xylopyranoside
-
isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40ưC
4.2
3-O-acetyl-4-nitrophenyl beta-D-xylopyranoside
-
in 50 mM citrate-phosphate (pH 6) at 70ưC
0.185 - 5.1
4-nitrophenyl acetate
0.057 - 7.1
4-nitrophenyl butyrate
0.245 - 0.355
4-nitrophenyl hexanoate
0.137
4-nitrophenyl propionate
-
in 50 mM citrate-phosphate (pH 6) at 70ưC
44.6
4-nitrophenyl-2-O-acetyl-alpha-L-arabinofuranoside
-
pH 5.5, 45ưC
3.28
4-nitrophenyl-2-O-acetyl-beta-D-xylopyranoside
-
pH 5.5, 45ưC
9.44
4-nitrophenyl-3-O-acetyl-beta-D-xylopyranoside
-
pH 5.5, 45ưC
6.78
4-nitrophenyl-4-O-acetyl-beta-D-xylopyranoside
-
pH 5.5, 45ưC
16.4
4-nitrophenyl-5-O-acetyl-alpha-L-arabinofuranoside
-
pH 5.5, 45ưC
0.75 - 2
4-nitrophenyl-butanoate
1.24 - 4.31
4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside
4
4-O-acetyl-4-nitrophenyl beta-D-xylopyranoside
-
in 50 mM citrate-phosphate (pH 6) at 70ưC
0.5
6-acetylmorphine
-
recombinant enzyme, pH 8.0
2.7 - 7.8
Alpha-naphthyl acetate
9.1
Cellulose acetate
-
pH 5.0, 45ưC
-
90
glyceryl triacetate
-
-
0.15
methylumbelliferone acetate
-
-
0.0067 - 385
p-nitrophenyl acetate
0.07
phenyl acetate
-
recombinant enzyme, pH 6.4
0.06
2-benzylcyclohex-1-en-1-yl acetate

-
isoenzyme Est II, pH 7.0, 35ưC
0.97
2-benzylcyclohex-1-en-1-yl acetate
-
isoenzyme Est I, pH 7.0, 35ưC
0.3
2-n-pentylcyclohex-1-en-1-yl acetate

-
isoenzyme Est II, pH 7.0, 35ưC
0.72
2-n-pentylcyclohex-1-en-1-yl acetate
-
isoenzyme Est I, pH 7.0, 35ưC
2.01
2-t-butylcyclohex-1-en-1-yl acetate

-
isoenzyme Est II, pH 7.0, 35ưC
2.54
2-t-butylcyclohex-1-en-1-yl acetate
-
isoenzyme Est I, pH 7.0, 35ưC
0.185
4-nitrophenyl acetate

-
in 50 mM citrate-phosphate (pH 6) at 70ưC
0.23
4-nitrophenyl acetate
30ưC, pH 5.8
0.23
4-nitrophenyl acetate
purified recombinant enzyme, 30ưC, pH 5.8
0.285
4-nitrophenyl acetate
-
at pH 8.0 and 20ưC
0.84
4-nitrophenyl acetate
at pH 7.0 and 39ưC
1.1
4-nitrophenyl acetate
-
wild type enzyme, at pH 5.5 and 50ưC
1.4
4-nitrophenyl acetate
-
mutant enzyme D179A, at pH 5.5 and 50ưC
1.8
4-nitrophenyl acetate
-
mutant enzyme H182A, at pH 5.5 and 50ưC
3.76
4-nitrophenyl acetate
-
at pH 8.0 and 35ưC
4.7
4-nitrophenyl acetate
-
mutant enzyme C16A, at pH 5.5 and 50ưC
5.1
4-nitrophenyl acetate
-
mutant enzyme S10A, at pH 5.5 and 50ưC
0.057
4-nitrophenyl butyrate

-
mutant enzyme L209F, in 20 mM phosphate buffer, at pH 7.1 and 30ưC
0.069
4-nitrophenyl butyrate
-
mutant enzyme L97F/L209F, in 20 mM phosphate buffer, at pH 7.1 and 30ưC
0.13
4-nitrophenyl butyrate
mutant enzyme R48E, in 20 mM phosphate buffer (pH 7.1), at 30ưC
0.14
4-nitrophenyl butyrate
mutant enzyme R48K, in 20 mM phosphate buffer (pH 7.1), at 30ưC
0.16
4-nitrophenyl butyrate
wild type enzyme, in 20 mM phosphate buffer (pH 7.1), at 30ưC
0.17
4-nitrophenyl butyrate
-
wild type enzyme, in 20 mM phosphate buffer, at pH 7.1 and 30ưC
0.18
4-nitrophenyl butyrate
mutant enzyme R48A, in 20 mM phosphate buffer (pH 7.1), at 30ưC
0.19
4-nitrophenyl butyrate
mutant enzyme R48S, in 20 mM phosphate buffer (pH 7.1), at 30ưC
0.26
4-nitrophenyl butyrate
-
mutant enzyme L97F, in 20 mM phosphate buffer, at pH 7.1 and 30ưC
7.1
4-nitrophenyl butyrate
-
wild type enzyme, at pH 5.5 and 50ưC
0.245
4-nitrophenyl hexanoate

-
pH 7.1, 65ưC, recombinant wild-type enzyme
0.355
4-nitrophenyl hexanoate
-
pH 7.1, 65ưC, recombinant mutant T74A
0.75
4-nitrophenyl-butanoate

-
pH 7.1, 25ưC, recombinant wild-type enzyme
1.5
4-nitrophenyl-butanoate
-
pH 7.1, 65ưC, recombinant wild-type enzyme
2
4-nitrophenyl-butanoate
-
pH 7.1, 65ưC, recombinant mutant T74A
1.24
4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside

-
isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40ưC
2.46
4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside
-
isozyme CE2, in 0.1 M sodium phosphate buffer, pH 6.0, at 40ưC
4.31
4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside
-
isozyme CE2C, in 0.1 M sodium phosphate buffer, pH 6.0, at 40ưC
2.7
Alpha-naphthyl acetate

-
-
7.8
Alpha-naphthyl acetate
-
pH 6.5
0.0067
p-nitrophenyl acetate

-
-
0.023
p-nitrophenyl acetate
-
-
0.43
p-nitrophenyl acetate
-
pH 6.5
0.9
p-nitrophenyl acetate
-
-
32.5
p-nitrophenyl acetate
-
pH 7.0, 25ưC, presence of benzethonium chloride
385
p-nitrophenyl acetate
-
pH 7.0, 25ưC, absence of benzethonium chloride
0.015
tributyrin

mutant enzyme R48S, in 20 mM phosphate buffer (pH 7.1), at 30ưC
0.018
tributyrin
mutant enzyme R48A, in 20 mM phosphate buffer (pH 7.1), at 30ưC
0.047
tributyrin
mutant enzyme R48K, in 20 mM phosphate buffer (pH 7.1), at 30ưC
0.052
tributyrin
wild type enzyme, in 20 mM phosphate buffer (pH 7.1), at 30ưC
0.073
tributyrin
mutant enzyme R48E, in 20 mM phosphate buffer (pH 7.1), at 30ưC
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20 - 33
2-benzylcyclohex-1-en-1-yl acetate
63
2-ethylcyclohex-1-en-1-yl acetate
-
isoenzyme Est I, pH 7.0, 35ưC
14
2-isopropylcyclohex-1-en-1-yl acetate
-
isoenzyme Est I, pH 7.0, 35ưC
77
2-methylcyclohex-1-en-1-yl acetate
-
isoenzyme Est I, pH 7.0, 35ưC
26 - 42
2-n-pentylcyclohex-1-en-1-yl acetate
79
2-n-propylcyclohex-1-en-1-yl acetate
-
isoenzyme Est I, pH 7.0, 35ưC
41.6
2-O-acetyl 4-nitrophenyl beta-D-xylopyranoside
-
isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40ưC
76.1
2-O-acetyl-4-nitrophenyl beta-D-xylopyranoside
-
in 50 mM citrate-phosphate (pH 6) at 70ưC
7 - 17
2-t-butylcyclohex-1-en-1-yl acetate
2101
3-O-acetyl 4-nitrophenyl beta-D-xylopyranoside
-
isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40ưC
70.1
3-O-acetyl-4-nitrophenyl beta-D-xylopyranoside
-
in 50 mM citrate-phosphate (pH 6) at 70ưC
0.82 - 57.5
4-nitrophenyl acetate
29 - 340
4-nitrophenyl butyrate
34.5 - 48.8
4-nitrophenyl hexanoate
41.3
4-nitrophenyl propionate
-
in 50 mM citrate-phosphate (pH 6) at 70ưC
0.15
4-nitrophenyl-2-O-acetyl-alpha-L-arabinofuranoside
-
pH 5.5, 45ưC
3.23
4-nitrophenyl-2-O-acetyl-beta-D-xylopyranoside
-
pH 5.5, 45ưC
1.9
4-nitrophenyl-3-O-acetyl-beta-D-xylopyranoside
-
pH 5.5, 45ưC
0.91
4-nitrophenyl-4-O-acetyl-beta-D-xylopyranoside
-
pH 5.5, 45ưC
1.77
4-nitrophenyl-5-O-acetyl-alpha-L-arabinofuranoside
-
pH 5.5, 45ưC
21 - 155
4-nitrophenyl-butanoate
241.6 - 3210
4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside
78.6
4-O-acetyl-4-nitrophenyl beta-D-xylopyranoside
-
in 50 mM citrate-phosphate (pH 6) at 70ưC
12.6
6-acetylmorphine
-
recombinant enzyme, pH 8.0
3
phenyl acetate
-
recombinant enzyme, pH 6.4
additional information
4-nitrophenyl-beta-D-xylopyranoside monoacetate
initial rate ratio between 2-, 3-, and 4-O-acetyl 4-nitrophenyl-beta-D-xylopyranoside is 1:19:17.7
20
2-benzylcyclohex-1-en-1-yl acetate

-
isoenzyme Est I, pH 7.0, 35ưC
33
2-benzylcyclohex-1-en-1-yl acetate
-
isoenzyme Est II, pH 7.0, 35ưC
26
2-n-pentylcyclohex-1-en-1-yl acetate

-
isoenzyme Est II, pH 7.0, 35ưC
42
2-n-pentylcyclohex-1-en-1-yl acetate
-
isoenzyme Est I, pH 7.0, 35ưC
7
2-t-butylcyclohex-1-en-1-yl acetate

-
isoenzyme Est I, pH 7.0, 35ưC
17
2-t-butylcyclohex-1-en-1-yl acetate
-
isoenzyme Est II, pH 7.0, 35ưC
0.82
4-nitrophenyl acetate

at pH 7.0 and 39ưC
5.5
4-nitrophenyl acetate
-
at pH 8.0 and 20ưC
21.8
4-nitrophenyl acetate
-
mutant enzyme S10A, at pH 5.5 and 50ưC
26.2
4-nitrophenyl acetate
-
mutant enzyme C16A, at pH 5.5 and 50ưC
36.3
4-nitrophenyl acetate
-
mutant enzyme D179A, at pH 5.5 and 50ưC
38.8
4-nitrophenyl acetate
-
mutant enzyme H182A, at pH 5.5 and 50ưC
49.2
4-nitrophenyl acetate
-
wild type enzyme, at pH 5.5 and 50ưC
57.5
4-nitrophenyl acetate
-
in 50 mM citrate-phosphate (pH 6) at 70ưC
29
4-nitrophenyl butyrate

mutant enzyme R48K, in 20 mM phosphate buffer (pH 7.1), at 30ưC
29
4-nitrophenyl butyrate
wild type enzyme, in 20 mM phosphate buffer (pH 7.1), at 30ưC
29
4-nitrophenyl butyrate
-
wild type enzyme, in 20 mM phosphate buffer, at pH 7.1 and 30ưC
29.8
4-nitrophenyl butyrate
-
wild type enzyme, at pH 5.5 and 50ưC
37
4-nitrophenyl butyrate
-
mutant enzyme L209F, in 20 mM phosphate buffer, at pH 7.1 and 30ưC
78
4-nitrophenyl butyrate
mutant enzyme R48E, in 20 mM phosphate buffer (pH 7.1), at 30ưC
86
4-nitrophenyl butyrate
mutant enzyme R48S, in 20 mM phosphate buffer (pH 7.1), at 30ưC
113
4-nitrophenyl butyrate
mutant enzyme R48A, in 20 mM phosphate buffer (pH 7.1), at 30ưC
240
4-nitrophenyl butyrate
-
mutant enzyme L97F, in 20 mM phosphate buffer, at pH 7.1 and 30ưC
340
4-nitrophenyl butyrate
-
mutant enzyme L97F/L209F, in 20 mM phosphate buffer, at pH 7.1 and 30ưC
34.5
4-nitrophenyl hexanoate

-
pH 7.1, 65ưC, recombinant wild-type enzyme
48.8
4-nitrophenyl hexanoate
-
pH 7.1, 65ưC, recombinant mutant T74A
21
4-nitrophenyl-butanoate

-
pH 7.1, 25ưC, recombinant wild-type enzyme
145
4-nitrophenyl-butanoate
-
pH 7.1, 65ưC, recombinant wild-type enzyme
155
4-nitrophenyl-butanoate
-
pH 7.1, 65ưC, recombinant mutant T74A
241.6
4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside

-
isozyme CE2C, in 0.1 M sodium phosphate buffer, pH 6.0, at 40ưC
1331
4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside
-
isozyme CE2, in 0.1 M sodium phosphate buffer, pH 6.0, at 40ưC
3210
4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside
-
isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40ưC
6.2
tributyrin

wild type enzyme, in 20 mM phosphate buffer (pH 7.1), at 30ưC
6.9
tributyrin
mutant enzyme R48K, in 20 mM phosphate buffer (pH 7.1), at 30ưC
9.3
tributyrin
mutant enzyme R48E, in 20 mM phosphate buffer (pH 7.1), at 30ưC
17.4
tributyrin
mutant enzyme R48A, in 20 mM phosphate buffer (pH 7.1), at 30ưC
17.5
tributyrin
mutant enzyme R48S, in 20 mM phosphate buffer (pH 7.1), at 30ưC
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0.000109
4-nitrophenyl butyrate, purified recombinant enzyme
0.000213
4-nitrophenyl formate, purified recombinant enzyme
0.000225
-
mutant strain, ethyl acetate-hydrolysing esterase (EAHase) activity
0.000236
-
mutant strain, isoamyl acetate-hydrolysing esterase (IAHase) activity
0.000342
-
wild-type strain, ethyl acetate-hydrolysing esterase (EAHase) activity
0.000347
-
wild-type strain, isoamyl acetate-hydrolysing esterase (IAHase) activity
0.00507
-
with 4-nitrophenyl butyrate as substrate, at pH 6.0 and 35ưC
0.021
Candida bororiensis
-
-
0.027
-
purified native enzyme, substrate ragi
0.061
-
purified native enzyme, substrate larch wood xylan
0.082
-
substrate p-nitrophenyl laurate, pH 7.0, 25ưC
0.251
-
substrate p-nitrophenyl acetate, pH 7.0, 25ưC
0.282
-
purified native enzyme, substrate gum karaya
0.297
-
substrate p-nitrophenyl decanoate, pH 7.0, 25ưC
0.352
-
substrate p-nitrophenyl hexanoate, pH 7.0, 25ưC
0.369
-
substrate p-nitrophenyl propionate, pH 7.0, 25ưC
0.474
-
substrate p-nitrophenyl butyrate, pH 7.0, 25ưC
0.52
-
crude enzyme, at pH 8.0 and 35ưC
0.85
recombinant enzyme, after CM Sepharose FF column
1.12
recombinant enzyme, after DEAE FF column, 4-nitrophenyl acetate
1.21
4-nitrophenyl acetate, purified recombinant enzyme
10.3
-
with 4-nitrophenyl 2-O-acetyl-beta-D-xylopyranoside as substrate, at pH 6.0 and 35ưC
1027
-
alpha-naphthyl acetate, pH 6.5
104.85
-
with 4-nitrophenyl acetate as substrate, at pH 6.0 and 35ưC
13.3
-
with 4-nitrophenyl 3-O-acetyl-beta-D-xylopyranoside as substrate, at pH 6.0 and 35ưC
2.37
-
with 4-nitrophenyl propionate as substrate, at pH 6.0 and 35ưC
20.5
4-nitrophenyl acetate, maximum velocity, purified recombinant enzyme, 30ưC, pH 5.8
221.9
-
after 423.39fold purification, at pH 8.0 and 35ưC
250
-
pH 7.1, 65ưC, purified recombinant wild-type enzyme
270
-
pH 7.1, 65ưC, purified recombinant mutant T74A
3.38
-
purified native enzyme, substrate alpha-naphthyl acetate
324
-
(R,S)-1,2-O-isopropylidene glycol acetate, EST1, after preparative electrophoresis
328
-
isozyme CE2B, using methyl 6-O-acetyl-beta-D-glucopyranoside as substrate, pH 6.0, 40ưC
333
-
wild-type, using 4-nitrophenyl acetate as substrate
35
-
isozyme CE2, using 6-O-acetyl-D-mannopyranose as substrate, pH 6.0, 40ưC
395
-
isozyme CE2B, using 6-O-acetyl-D-mannopyranose as substrate, pH 6.0, 40ưC
4
-
isozyme CE2B, using methyl 3,4-O-diacetyl-beta-D-xylopyranoside as substrate, pH 6.0, 40ưC
4.44
-
purified native enzyme, substrate wheat
46.5
-
mutant E188W/M193C, using 4-nitrophenyl acetate as substrate
5.39
-
purified native enzyme, substrate 4-nitrophenyl acetate
5.64
-
with 4-nitrophenyl 4-O-acetyl-beta-D-xylopyranoside as substrate, at pH 6.0 and 35ưC
60
-
isozyme CE2C, using methyl 6-O-acetyl-beta-D-glucopyranoside as substrate, pH 6.0, 40ưC
71
-
p-nitrophenyl acetate, pH 6.5
8
-
isozyme CE2, using methyl 3,4-O-diacetyl-beta-D-xylopyranoside as substrate, pH 6.0, 40ưC
9.63
-
p-nitrophenyl acetate, after hydroxyapatite chromatography, pH 6.5
0.21

4-nitrophenyl acetate, over-expression culture supernatant
0.21
recombinant enzyme, culture supernatant
183

-
isozyme CE2, using methyl 6-O-acetyl-beta-D-glucopyranoside as substrate, pH 6.0, 40ưC
183
-
isozyme CE2, using methyl 6-O-acetyl-beta-D-glucopyranoside as substrate, pH 6.0, 40ưC
2

-
isozyme CE2C, using methyl 2,4-O-diacetyl-beta-D-xylopyranoside as substrate, pH 6.0, 40ưC
2
-
isozyme CE2, using methyl 2,4-O-diacetyl-beta-D-xylopyranoside as substrate, pH 6.0, 40ưC
3

-
isozyme CE2C, using 6-O-acetyl-D-mannopyranose as substrate, pH 6.0, 40ưC
3
-
isozyme CE2C, using methyl 3,4-O-diacetyl-beta-D-xylopyranoside as substrate, pH 6.0, 40ưC
5

-
-
5
-
isozyme CE2B, using methyl 2,4-O-diacetyl-beta-D-xylopyranoside as substrate, pH 6.0, 40ưC
additional information

-
substrate specificity
additional information
-
-
additional information
-
-
additional information
-
isoforms with pI-values 4.2 and 5.0 are the most active ones in all samples
additional information
-
(R)-1,2-O-isopropylidene glycol acetate, EST1, 1.0 micromol/min by activity corresponding to synthesis of 0.01 micromol p-nitrophenol/min/mg EST1
additional information
-
(R,S)-1,2-O-isopropylidene glycol acetate as substrate for EST1, 20 micromol/min by activity corresponding to synthesis of 0.01 micromol p-nitrophenol/min/mg EST1
additional information
-
(R,S)-1,2-O-isopropylidene glycol butyrate as substrate for EST1, 4.6 micromol/min by activity corresponding to synthesis of 0.01 micromol p-nitrophenol/min/mg EST1
additional information
-
(R,S)-1,2-O-isopropylidene glycol caproate as substrate for EST1, 0.7 micromol/min by activity corresponding to synthesis of 0.01 micromol p-nitrophenol/min/mg EST1
additional information
-
(S)-1,2-O-isopropylidene glycol acetate, EST1, 1.2 micromol/min by activity corresponding to synthesis of 0.01 micromol p-nitrophenol/min/mg EST1
additional information
-
triacetin as substrate for EST1, 4.06 micromol/min by activity corresponding to synthesis of 0.01 micromol p-nitrophenol/min/mg EST1
additional information
-
tributyrin as substrate for EST1, 2.8 micromol/min by activity corresponding to synthesis of 0.01 micromol p-nitrophenol/min/mg EST1
additional information
extremly low activities against 4-nitrophenyl formate, butyrate, or canoate
additional information
-
mutant strain, 61% lower ethyl acetate-hydrolysing esterase (EAHase) activity than in wild-type strain
additional information
-
mutant strain, 68% lower isoamyl acetate-hydrolysing esterase (IAHase) activity than in wild-type strain
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