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Literature summary for 3.1.1.6 extracted from

  • Kobayashi, R.; Hirano, N.; Kanaya, S.; Saito, I.; Haruki, M.
    Enhancement of the enzymatic activity of Escherichia coli acetyl esterase by random mutagenesis (2010), J. Mol. Catal. B, 67, 155-161.
No PubMed abstract available
Search for more literature for 3.1.1.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109 cells Escherichia coli

Protein Variants

Protein Variants Comment Organism
R48A the enzymatic activity of the mutant is enhanced by improvement of catalytic efficiency (3.7fold with 4-nitrophenyl butyrate and 8fold with tributyrin as substrate) Escherichia coli
R48E the enzymatic activity of the mutant is enhanced by improvement of catalytic efficiency (3.4fold with 4-nitrophenyl butyrate and 1.3fold with tributyrin as substrate) Escherichia coli
R48K the catalytic efficiency of the mutant is similar to the wild type enzyme Escherichia coli
R48S the enzymatic activity of the mutant is enhanced by improvement of catalytic efficiency (2.6fold with 4-nitrophenyl butyrate and 9.7fold with tributyrin as substrate) Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 tributyrin mutant enzyme R48S, in 20 mM phosphate buffer (pH 7.1), at 30°C Escherichia coli
0.018
-
 tributyrin mutant enzyme R48A, in 20 mM phosphate buffer (pH 7.1), at 30°C Escherichia coli
0.047
-
 tributyrin mutant enzyme R48K, in 20 mM phosphate buffer (pH 7.1), at 30°C Escherichia coli
0.052
-
 tributyrin wild type enzyme, in 20 mM phosphate buffer (pH 7.1), at 30°C Escherichia coli
0.073
-
 tributyrin mutant enzyme R48E, in 20 mM phosphate buffer (pH 7.1), at 30°C Escherichia coli
0.13
-
 4-nitrophenyl butyrate mutant enzyme R48E, in 20 mM phosphate buffer (pH 7.1), at 30°C Escherichia coli
0.14
-
 4-nitrophenyl butyrate mutant enzyme R48K, in 20 mM phosphate buffer (pH 7.1), at 30°C Escherichia coli
0.16
-
 4-nitrophenyl butyrate wild type enzyme, in 20 mM phosphate buffer (pH 7.1), at 30°C Escherichia coli
0.18
-
 4-nitrophenyl butyrate mutant enzyme R48A, in 20 mM phosphate buffer (pH 7.1), at 30°C Escherichia coli
0.19
-
 4-nitrophenyl butyrate mutant enzyme R48S, in 20 mM phosphate buffer (pH 7.1), at 30°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P23872
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl butyrate + H2O
-
 Escherichia coli 4-nitrophenol + butyrate
-
 ?
tributyrin + H2O
-
 Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
Acetyl esterase
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.2
-
 tributyrin wild type enzyme, in 20 mM phosphate buffer (pH 7.1), at 30°C Escherichia coli
6.9
-
 tributyrin mutant enzyme R48K, in 20 mM phosphate buffer (pH 7.1), at 30°C Escherichia coli
9.3
-
 tributyrin mutant enzyme R48E, in 20 mM phosphate buffer (pH 7.1), at 30°C Escherichia coli
17.4
-
 tributyrin mutant enzyme R48A, in 20 mM phosphate buffer (pH 7.1), at 30°C Escherichia coli
17.5
-
 tributyrin mutant enzyme R48S, in 20 mM phosphate buffer (pH 7.1), at 30°C Escherichia coli
29
-
 4-nitrophenyl butyrate mutant enzyme R48K, in 20 mM phosphate buffer (pH 7.1), at 30°C Escherichia coli
29
-
 4-nitrophenyl butyrate wild type enzyme, in 20 mM phosphate buffer (pH 7.1), at 30°C Escherichia coli
78
-
 4-nitrophenyl butyrate mutant enzyme R48E, in 20 mM phosphate buffer (pH 7.1), at 30°C Escherichia coli
86
-
 4-nitrophenyl butyrate mutant enzyme R48S, in 20 mM phosphate buffer (pH 7.1), at 30°C Escherichia coli
113
-
 4-nitrophenyl butyrate mutant enzyme R48A, in 20 mM phosphate buffer (pH 7.1), at 30°C Escherichia coli