EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
    2.4.1.18 | 60 |
- |
the wild type enzyme shows a half-life of 42.1 min at 60°C and retains less than 20% activity after 2 h at 60°C. Mutant enzyme Q231R shows a half-life of 53.2 min at 60°C and retains about 31% activity after 2 h at 60°C. Mutant enzyme Q231K shows a half-life of 53.6 min at 60°C and retains about 33% activity after 2 h at 60°C. Mutant enzyme T339E shows a half-life of 49.1 min at 60°C and retains about 28% activity after 2 h at 60°C. Mutant enzyme T339D shows a half-life of 50.4 min at 60°C and retains about 23% activity after 2 h at 60°C. Mutant enzyme I571D shows a half-life of 64.2 min at 60°C and retains about 36% activity after 2 h at 60°C |
756760 |
| 2.4.1.18 | 60 |
65 |
after 1 h incubation at 60°C fully active, after 1 h incubation at 65°C only 80% residual activity |
701786 |
| 2.4.1.18 | 60 |
70 |
the half-life of the enzyme at 60 and 70°C is 24 h and 5 h, respectively |
757910 |
| 2.4.1.18 | 70 |
- |
pH 7.0, 30 min, 10% loss of the soluble enzyme form, 50% loss of the insoluble enzyme form |
636942 |
| 2.4.1.18 | 70 |
90 |
the enzyme remains stable at temperatures up to 80°C and has a half-life at 85°C of approximately 31 min. The enzyme is quite stable (for at least 2 h) at 70°C or lower, and 92% of the initial enzyme activity is retained at 80°C for 2 h. Furthermore, the enzyme demonstrates half-lives at 85 and 90°C of 31 min and 2.5 min, respectively |
758278 |
| 2.4.1.18 | 75 |
- |
fully active after 100 min incubation, half-life time at 80°C 73.7 min, half-life time at 85°C 16.7 min |
705085 |
| 2.4.1.18 | 85 |
- |
soluble and insoluble enzyme form, stable up to |
636942 |
| 2.4.1.18 | 90 |
- |
the protein is stable up to 90°C, the activity decreases gradually during the incubation at 100°C |
674295 |
