2.4.1.18	additional information	-	high thermal tolerance even at boiling temperatures	721012	
2.4.1.18	additional information	-	the activity is lost after incubation at 50°C for 30 min	674221	
2.4.1.18	additional information	-	when the enzymes are maintained for 15 min at various temperatures ranging from 30°C to 60°C, more than 80% of the original activity was retained up to 47.5°C for PvSBE2, up to 40°C for D15A- and D15E-PvSBE2, up to 42.5°C for R28A- and H24A-PvSBE2, and up to 45°C for R28K-PvSBE2.	676385	
2.4.1.18	-	45	the enzyme retains about 80% of its activity at 0-45° for 1 h	756764	
2.4.1.18	5	45	no apparent inactivation up to 30°C, about 10% loss of activity at 35°C, about 90% loss of enzyme activity at 50°C	705081	
2.4.1.18	10	30	pre-incubation of the enzyme for 2 h at different temperatures, following enzyme assay is conducted at 30°C. About 80% activity is retained after pre-incubation at 37°C and 45°C	676973	
2.4.1.18	10	40	purified recombinant enzyme, the enzyme retains about 80% of its activity at 40°C for 30 min, the enzyme displays excellent cold adaptation over a low temperature range of 10-30°C, retaining over 85% of its relative activity within this range	736135	
2.4.1.18	35	-	pH 8.0, 30 min, stable below	636932	
2.4.1.18	35	-	purified recombinant His-tagged isozyme BE3, stable up to	735716	
2.4.1.18	35	-	the half-life of the enzyme at 35°C is 10 h	756961	
2.4.1.18	40	-	above 40°C enzyme inactivated after incubation time of 1 h	703793	
2.4.1.18	40	-	pH 8.0, 15 min, stable up to, enzyme form KBE1	636945, 636954	
2.4.1.18	40	-	purified recombinant His-tagged isozyme BE1, stable up to	735716	
2.4.1.18	45	-	purified recombinant His-tagged isozyme BE2, stable up to	735716	
2.4.1.18	45	-	with a direct enzyme assay at 45°C the enzyme retains about 45% activity	676973	
2.4.1.18	50	-	after 1 h incubation at fully active	701786	
2.4.1.18	50	-	pH 8.0, 15 min, stable up to, enzyme form KBE2	636954	
2.4.1.18	50	55	in the absence of substrates the branching enzyme is stable at 50°C for more than 6 h. At 55°C the half-life of the branching enzyme is 50 min.	671536	
2.4.1.18	50	60	the wild type enzyme retains 100, about 75 and about 25% activity after 15 min at 50, 60 and 65°C, respectively. The enzyme is inactive after 15 min at 70°C. Its melting temperature is 63°C	756935	
2.4.1.18	51	-	partial inactivation	636938	
2.4.1.18	60	-	the wild type enzyme shows a half-life of 42.1 min at 60°C and retains less than 20% activity after 2 h at 60°C. Mutant enzyme Q231R shows a half-life of 53.2 min at 60°C and retains about 31% activity after 2 h at 60°C. Mutant enzyme Q231K shows a half-life of 53.6 min at 60°C and retains about 33% activity after 2 h at 60°C. Mutant enzyme T339E shows a half-life of 49.1 min at 60°C and retains about 28% activity after 2 h at 60°C. Mutant enzyme T339D shows a half-life of 50.4 min at 60°C and retains about 23% activity after 2 h at 60°C. Mutant enzyme I571D shows a half-life of 64.2 min at 60°C and retains about 36% activity after 2 h at 60°C	756760	
2.4.1.18	60	65	after 1 h incubation at 60°C fully active, after 1 h incubation at 65°C only 80% residual activity	701786	
2.4.1.18	60	70	the half-life of the enzyme at 60 and 70°C is 24 h and 5 h, respectively	757910	
2.4.1.18	70	-	pH 7.0, 30 min, 10% loss of the soluble enzyme form, 50% loss of the insoluble enzyme form	636942	
2.4.1.18	70	90	the enzyme remains stable at temperatures up to 80°C and has a half-life at 85°C of approximately 31 min. The enzyme is quite stable (for at least 2 h) at 70°C or lower, and 92% of the initial enzyme activity is retained at 80°C for 2 h. Furthermore, the enzyme demonstrates half-lives at 85 and 90°C of 31 min and 2.5 min, respectively	758278	
2.4.1.18	75	-	fully active after 100 min incubation, half-life time at 80°C 73.7 min, half-life time at 85°C 16.7 min	705085	
2.4.1.18	85	-	soluble and insoluble enzyme form, stable up to	636942	
2.4.1.18	90	-	the protein is stable up to 90°C, the activity decreases gradually during the incubation at 100°C	674295