EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
    2.4.1.18 | 40 |
- |
above 40°C enzyme inactivated after incubation time of 1 h |
703793 |
| 2.4.1.18 | 40 |
- |
pH 8.0, 15 min, stable up to, enzyme form KBE1 |
636945, 636954 |
| 2.4.1.18 | 40 |
- |
purified recombinant His-tagged isozyme BE1, stable up to |
735716 |
| 2.4.1.18 | 45 |
- |
purified recombinant His-tagged isozyme BE2, stable up to |
735716 |
| 2.4.1.18 | 45 |
- |
with a direct enzyme assay at 45°C the enzyme retains about 45% activity |
676973 |
| 2.4.1.18 | 50 |
- |
after 1 h incubation at fully active |
701786 |
| 2.4.1.18 | 50 |
- |
pH 8.0, 15 min, stable up to, enzyme form KBE2 |
636954 |
| 2.4.1.18 | 50 |
55 |
in the absence of substrates the branching enzyme is stable at 50°C for more than 6 h. At 55°C the half-life of the branching enzyme is 50 min. |
671536 |
| 2.4.1.18 | 50 |
60 |
the wild type enzyme retains 100, about 75 and about 25% activity after 15 min at 50, 60 and 65°C, respectively. The enzyme is inactive after 15 min at 70°C. Its melting temperature is 63°C |
756935 |
| 2.4.1.18 | 51 |
- |
partial inactivation |
636938 |
