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Literature summary for 2.4.1.18 extracted from
- Thermostabilization of a thermophilic 1,4-alpha-glucan branching enzyme through C-terminal truncation (2018), Int. J. Biol. Macromol., 107, 1510-1518 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Parageobacillus thermoglucosidasius |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | compared with the wild type enzyme, a truncation mutant made by removing the last 26 residues from its C-terminal end has enhanced thermostability and recovery ability without compromising enzymatic activity | Parageobacillus thermoglucosidasius |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Parageobacillus thermoglucosidasius | A0A068F9H3 | - |
- |
| Parageobacillus thermoglucosidasius STB02 | A0A068F9H3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HiTrap ANX column chromatography | Parageobacillus thermoglucosidasius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylose | - |
Parageobacillus thermoglucosidasius | amylose containing alpha-1,6-glucosidic linkages | - |
? |  |
| amylose | - |
Parageobacillus thermoglucosidasius STB02 | amylose containing alpha-1,6-glucosidic linkages | - |
? | |
| soluble starch | - |
Parageobacillus thermoglucosidasius | soluble starch containing alpha-1,6-glucosidic linkages | - |
? | |
| soluble starch | - |
Parageobacillus thermoglucosidasius STB02 | soluble starch containing alpha-1,6-glucosidic linkages | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GBE | - |
Parageobacillus thermoglucosidasius |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Parageobacillus thermoglucosidasius |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 60 | the wild type enzyme retains 100, about 75 and about 25% activity after 15 min at 50, 60 and 65°C, respectively. The enzyme is inactive after 15 min at 70°C. Its melting temperature is 63°C | Parageobacillus thermoglucosidasius |
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Release 2023.1 (January 2023)
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