3.4.24.B7 | solubilization of recombinant proMMP-26 from Escherichia coli inclusion bodies by 8 M urea, followed by dialysis against 4 M urea, 1 mM DTT, and 50 mM HEPES or Tricine at pH 7.5 for at least 1 h, and then folded by dialysis against buffer containing 50 mM Hepes, 0.2 M, NaCl, 10 mM CaCl2, 20 lM ZnSO4, and 0.05% Brij-35 at pH 7.5 for 12 h three times, MMP-26 autoactivates |
707115 |