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Literature summary for 3.4.24.B7 extracted from

  • Park, H.I.; Lee, S.; Ullah, A.; Cao, Q.; Sang, Q.X.
    Effects of detergents on catalytic activity of human endometase/matrilysin 2, a putative cancer biomarker (2010), Anal. Biochem., 396, 262-268.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.B7

Activating Compound

Activating Compound Comment Organism Structure
Brij-35 optimally activating at 0.056 mM, at concentrations above the critical micelle concentration, it inhibits the enzyme noncompetitively at 0.05%, i.e. 0.417 mM Homo sapiens
additional information the hydrolytic activity of endometase is detergent concentration dependent, exhibiting a bell-shaped curve with its maximum activity near the critical micelle concentration of nonionic detergents tested, overview. Monomer of detergents may activate and stabilize MMPs to enhance catalysis, but micelle of detergents may sequester enzyme and block the substrate binding site to impede catalysis Homo sapiens
SDS optimally activating at 0.0095 mM, at concentrations above the critical micelle concentration Homo sapiens
Tetradecyltrimethylammonium bromide optimally activating at 0.0075 mM, at concentrations above the critical micelle concentration Homo sapiens
Triton X-100 optimally activating at 0.140 mM, at concentrations above the critical micelle concentration Homo sapiens
Tween 20 optimally activating at 0.079 mM, at concentrations above the critical micelle concentration Homo sapiens

Application

Application Comment Organism
diagnostics MMP-26 is a putative early biomarker for human carcinomas Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of proMMP-26 in Escherichia coli in inclusion bodies Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Brij-35 optimally activating at 0.056 mM, at concentrations above the critical micelle concentration, it inhibits the enzyme noncompetitively at 0.05%, i.e. 0.417 mM Homo sapiens
additional information the hydrolytic activity of endometase is detergent concentration dependent, exhibiting a bell-shaped curve with its maximum activity near the critical micelle concentration of nonionic detergents tested, overview. Monomer of detergents may activate and stabilize MMPs to enhance catalysis, but micelle of detergents may sequester enzyme and block the substrate binding site to impede catalysis Homo sapiens
SDS optimally activating at 0.0095 mM, at concentrations above the critical micelle concentration Homo sapiens
Tetradecyltrimethylammonium bromide optimally activating at 0.0075 mM, at concentrations above the critical micelle concentration Homo sapiens
Triton X-100 optimally activating at 0.140 mM, at concentrations above the critical micelle concentration Homo sapiens
Tween 20 optimally activating at 0.079 mM, at concentrations above the critical micelle concentration Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification MMP-26 autoactivates Homo sapiens

Renatured (Commentary)

Renatured (Comment) Organism
solubilization of recombinant proMMP-26 from Escherichia coli inclusion bodies by 8 M urea, followed by dialysis against 4 M urea, 1 mM DTT, and 50 mM HEPES or Tricine at pH 7.5 for at least 1 h, and then folded by dialysis against buffer containing 50 mM Hepes, 0.2 M, NaCl, 10 mM CaCl2, 20 lM ZnSO4, and 0.05% Brij-35 at pH 7.5 for 12 h three times, MMP-26 autoactivates Homo sapiens

Subunits

Subunits Comment Organism
More the prodomain of MMP-26 is necessary for proper folding of the enzyme. Homology-modeling of the structure of the MMP-26 catalytic domain, overview Homo sapiens

Synonyms

Synonyms Comment Organism
endometase
-
 Homo sapiens
matrilysin 2
-
 Homo sapiens
MMP-26
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information detergent inhibition kinetics, overview Homo sapiens
0.24
-
 Brij-35 pH 7.5, 25°C Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.079
-
 pH 7.5, 25°C Homo sapiens Tetradecyltrimethylammonium bromide
0.11
-
 pH 7.5, 25°C Homo sapiens SDS
0.56
-
 pH 7.5, 25°C Homo sapiens Brij-35
0.64
-
 pH 7.5, 25°C Homo sapiens Tween 20
1.3
-
 pH 7.5, 25°C Homo sapiens Triton X-100

General Information

General Information Comment Organism
additional information under physiological conditions, a lipid or membrane microenvironment may regulate enzymatic activity Homo sapiens