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Literature summary for 3.4.24.B7 extracted from
- Identification and characterization of human endometase (Matrix metalloproteinase-26) from endometrial tumor (2000), J. Biol. Chem., 275, 20540-20544.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA sequence determination and analysis, expression of the zymogen in Escherichia coli | Homo sapiens |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | zinc-binding motif VATHEIGHSLGLQH | Homo sapiens |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
x * 28000, recombinant zymogen, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| proteins + H2O | Homo sapiens | enzyme might be rsponsible for inactivation of cytokins and serpins | peptides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q9NRE1 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | propeptides sequence PHCGVPDGSD | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant from Escherichia coli, to homogeneity | Homo sapiens |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| recombinant zymogen from inclusion bodies expressed in Escherichia coli, partial activation of th proform during refolding | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| endometrial adenocarcinoma cell | gene expression | Homo sapiens | - |
| additional information | no expression in testis, heart, brain, lung, liver, thymus, and melanoma G361 cell | Homo sapiens | - |
| uterus | gene expression | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (7-methoxycoumarin-4-yl)acetyl-PKPLAL-Dpa-AR-NH2 + H2O | synthetic fluorogenic peptide derived of matrix metalloproteinases | Homo sapiens | ? | - |
? | |
| (7-methoxycoumarin-4-yl)acetyl-PLAQAV-Dpa-RSSSR-NH2 + H2O | synthetic fluorogenic peptide derived of tumor necrosis factor-alpha converting enzyme | Homo sapiens | ? | - |
? | |
| (7-methoxycoumarin-4-yl)acetyl-PLGL-Dpa-AR-NH2 + H2O | synthetic fluorogenic peptide derived of matrix metalloproteinases | Homo sapiens | ? | - |
? | |
| (7-methoxycoumarin-4-yl)acetyl-RPKPVE-norvaline-WMK-(2,4-dinitrophenyl)-NH2 + H2O | synthetic fluorogenic peptide derived of matrix metalloproteinases | Homo sapiens | ? | - |
? | |
| (7-methoxycoumarin-4-yl)acetyl-RPKPYA-norvaline-WMK-(2,4-dinitrophenyl)-NH2 + H2O | synthetic fluorogenic peptide derived of matrix metalloproteinases | Homo sapiens | ? | - |
? | |
| alpha1-proteinase inhibitor + H2O | - |
Homo sapiens | ? | - |
? | |
| additional information | no activity with collagens, laminin, elastin, beta-casein, plasminogen, soybean trypsin inhibitor, Bowman-Birk inhibitor | Homo sapiens | ? | - |
? | |
| proteins + H2O | - |
Homo sapiens | peptides | - |
? | |
| proteins + H2O | enzyme might be rsponsible for inactivation of cytokins and serpins | Homo sapiens | peptides | - |
? | |
| type I gelatin + H2O | - |
Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 28000, recombinant zymogen, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endometase | - |
Homo sapiens |
| M10.029 | Merops-ID | Homo sapiens |
| matrix metalloproteinase-26 | - |
Homo sapiens |
| MMP-26 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
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