EC Number   |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
|---|
   5.2.1.8 | 0.78 |
- |
N-succinyl-Ala-Leu-(cis)-Pro-Phe-p-nitroanilide |
pH 7.8, 15°C |
727584 |
| 5.2.1.8 | 3.45 |
- |
4-aminobenzoyl-Cys-Phe-(trans)-Pro-Val-Cys-(NO2)-Tyr-NH2 |
mutant enzyme G148D, with 50 mM dithiothreitol in 35 mM HEPES, pH 7.8, at 10°C |
715653 |
| 5.2.1.8 | 4.2 |
- |
N-succinyl-Ala-Phe-(cis)-Pro-Phe-4-nitroanilide |
at 10 °C in 35 mM sodium HEPES buffer, pH 7.8 |
714320 |
| 5.2.1.8 | 5.11 |
- |
4-aminobenzoyl-Cys-Lys-(trans)-Pro-Ala-Cys-(NO2)-Tyr-NH2 |
mutant enzyme, with 50 mM dithiothreitol in 35 mM HEPES, pH 7.8, at 10°C |
715653 |
| 5.2.1.8 | 6.7 |
- |
N-succinyl-Ala-Ala-(cis)-Pro-Phe-4-nitroanilide |
at 10 °C in 35 mM sodium HEPES buffer, pH 7.8 |
714320 |
| 5.2.1.8 | 7.4 |
- |
N-succinyl-Ala-Glu-(cis)-Pro-Phe-4-nitroanilide |
at 10 °C in 35 mM sodium HEPES buffer, pH 7.8 |
714320 |
| 5.2.1.8 | 25.9 |
- |
N-succinyl-Ala-Leu-(cis)-Pro-Phe-4-nitroanilide |
at 10 °C in 35 mM sodium HEPES buffer, pH 7.8 |
714320 |
| 5.2.1.8 | 30 |
- |
RNase T1 |
mutant Y15A, pH not specified in the publication, temperature not specified in the publication |
747952 |
| 5.2.1.8 | 42 |
- |
N-succinyl-Ala-Ala-(cis)-Pro-Phe-4-nitroanilide |
pH 8.0, 8.5°C |
749058 |
| 5.2.1.8 | 46 |
- |
RNase T1 |
mutant I65P, pH not specified in the publication, temperature not specified in the publication |
747215 |
