Protein Variants | Comment | Organism |
---|---|---|
G148D | the FkpA313 mutant protein, which contains a G148D replacement in the PPIase domain, shows only a very low in vitro PPIase activity with both peptides (the activity amounts to 0.4 and 0.2% that of wild type enzyme) | Escherichia coli |
additional information | the temperature-sensitive mutant FkpA43 confers 10% activity to colicin M at 30°C and no activity at 42°C, displays a 2fold lower PPIase activity than wild type FkpA for Phe-Pro-176-Val and a 5fold lower activity for Leu-Pro-260-Gly than for Phe-Pro-176-Val (measured at 10°C) | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
FK506 | - |
Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Escherichia coli | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
colicin M | Escherichia coli | - |
? | - |
? | |
colicin M | Escherichia coli AB2847 | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli AB2847 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA-agarose column chromatography and Superdex 75 gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-aminobenzoyl-Cys-Lys-(trans)-Pro-Ala-Cys-(NO2)-Tyr-NH2 | - |
Escherichia coli | 4-aminobenzoyl-Cys-Lys-(cis)-Pro-Ala-Cys-(NO2)-Tyr-NH2 | - |
? | |
4-aminobenzoyl-Cys-Lys-(trans)-Pro-Ala-Cys-(NO2)-Tyr-NH2 | - |
Escherichia coli AB2847 | 4-aminobenzoyl-Cys-Lys-(cis)-Pro-Ala-Cys-(NO2)-Tyr-NH2 | - |
? | |
4-aminobenzoyl-Cys-Lys-(trans)-Pro-Gly-Cys-(NO2)-Tyr-NH2 | - |
Escherichia coli | 4-aminobenzoyl-Cys-Lys-(cis)-Pro-Gly-Cys-(NO2)-Tyr-NH2 | - |
? | |
4-aminobenzoyl-Cys-Lys-(trans)-Pro-Gly-Cys-(NO2)-Tyr-NH2 | - |
Escherichia coli AB2847 | 4-aminobenzoyl-Cys-Lys-(cis)-Pro-Gly-Cys-(NO2)-Tyr-NH2 | - |
? | |
4-aminobenzoyl-Cys-Phe-(trans)-Pro-Val-Cys-(NO2)-Tyr-NH2 | - |
Escherichia coli | 4-aminobenzoyl-Cys-Phe-(cis)-Pro-Val-Cys-(NO2)-Tyr-NH2 | - |
? | |
4-aminobenzoyl-Cys-Phe-(trans)-Pro-Val-Cys-(NO2)-Tyr-NH2 | - |
Escherichia coli AB2847 | 4-aminobenzoyl-Cys-Phe-(cis)-Pro-Val-Cys-(NO2)-Tyr-NH2 | - |
? | |
colicin M | - |
Escherichia coli | ? | - |
? | |
colicin M | - |
Escherichia coli AB2847 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FkpA | - |
Escherichia coli |
PPIase | - |
Escherichia coli |
prolyl cis-trans isomerase | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | colicin M unfolds during transfer across the outer or cytoplasmic membrane and refolds to the active form in the periplasm assisted by prolyl cis-trans isomerase/chaperone FkpA | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.45 | - |
4-aminobenzoyl-Cys-Phe-(trans)-Pro-Val-Cys-(NO2)-Tyr-NH2 | mutant enzyme G148D, with 50 mM dithiothreitol in 35 mM HEPES, pH 7.8, at 10°C | Escherichia coli | |
5.11 | - |
4-aminobenzoyl-Cys-Lys-(trans)-Pro-Ala-Cys-(NO2)-Tyr-NH2 | mutant enzyme, with 50 mM dithiothreitol in 35 mM HEPES, pH 7.8, at 10°C | Escherichia coli | |
96.9 | - |
4-aminobenzoyl-Cys-Lys-(trans)-Pro-Gly-Cys-(NO2)-Tyr-NH2 | wild type enzyme, with 50 mM dithiothreitol in 35 mM HEPES, pH 7.8, at 10°C | Escherichia coli | |
115 | - |
4-aminobenzoyl-Cys-Lys-(trans)-Pro-Ala-Cys-(NO2)-Tyr-NH2 | wild type enzyme, with 50 mM dithiothreitol in 35 mM HEPES, pH 7.8, at 10°C | Escherichia coli | |
1380 | - |
4-aminobenzoyl-Cys-Phe-(trans)-Pro-Val-Cys-(NO2)-Tyr-NH2 | wild type enzyme, with 50 mM dithiothreitol in 35 mM HEPES, pH 7.8, at 10°C | Escherichia coli |