Application | Comment | Organism |
---|---|---|
medicine | CYPJ expression is upregulated in over 60% hepatocellular carcinoma tissues. CYPJ is found in the whole cell of hepatocellular carcinoma tissues with preferential location at the cell nucleus | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
structure of CYPJ at 2.0 A and in complex with cyclosporin A. Residues His43, Arg44 and Gln52 are conserved active-site residues located in the shallow pocket, and Arg44 displayes flexible conformation | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K120A | 87% of wild-type activity | Homo sapiens |
R44A | 57% of wild-type activity | Homo sapiens |
R44A/F49A | 32% of wild-type activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cyclosporin A | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.814 | - |
N-succinyl-Ala-Ala-(cis)-Pro-Phe-4-nitroanilide | pH 8.0, 8.5°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | CYPJ is found in the whole cell of hepatocellular carcinoma tissues with preferential location at the cell nucleus | Homo sapiens | - |
- |
nucleus | CYPJ is found in the whole cell of hepatocellular carcinoma tissues with preferential location at the cell nucleus | Homo sapiens | 5634 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hepatoma cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-succinyl-Ala-Ala-(cis)-Pro-Phe-4-nitroanilide | - |
Homo sapiens | N-succinyl-Ala-Ala-(trans)-Pro-Phe-4-nitroanilide | - |
r |
Synonyms | Comment | Organism |
---|---|---|
cyclophilin J | - |
Homo sapiens |
CyPJ | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
34.2 | - |
N-succinyl-Ala-Ala-(cis)-Pro-Phe-4-nitroanilide | pH 8.0, 8.5°C | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.012 | - |
pH 8.0, 8.5°C | Homo sapiens | cyclosporin A |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | CYPJ expression is upregulated in over 60% hepatocellular carcinoma tissues | up |
General Information | Comment | Organism |
---|---|---|
physiological function | CYPJ promotes the transition of cells from G1 phase to S phase by activating cyclin D1 promoter. CYPJ overexpression accelerates liver cell growth in vitro and in vivo. Inhibition of CYPJ by cyclosporin A or CYPJ-specific RNAi diminishes the growth of liver cancer cells in vitro and in vivo | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
42 | - |
N-succinyl-Ala-Ala-(cis)-Pro-Phe-4-nitroanilide | pH 8.0, 8.5°C | Homo sapiens |