Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
A82P | mutation in hinge region, 41% of wild-type activity. Unlike wild-type, the mutant unfolds by a non-two state mechanism in the presence of urea. Mutation affects both the shape and size of the protein significantly | Escherichia coli |
I65P | mutation in hinge region, 6% of wild-type activity. Unlike wild-type, the mutant unfolds by a non-two state mechanism in the presence of urea. Mutation affects both the shape and size of the protein significantly | Escherichia coli |
V72P | mutation in hinge region, 60% of wild-type activity. Unlike wild-type, the mutant unfolds by a non-two state mechanism in the presence of urea | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54500 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A9L3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
RNAse T1 | RNase T1 refolding assay | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 23000, calculated from sequence | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
FKBP22 | - |
Escherichia coli |
FklB | - |
Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
46 | - |
RNase T1 | mutant I65P, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
338 | - |
RNase T1 | mutant A82P, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
491 | - |
RNase T1 | mutant V72P, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
818 | - |
RNase T1 | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli |