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Literature summary for 5.2.1.8 extracted from

  • Polley, S.; Chakravarty, D.; Chakrabarti, G.; Chattopadhyaya, R.; Sau, S.
    Proline substitutions in a Mip-like peptidyl-prolyl cis-trans isomerase severely affect its structure, stability, shape and activity (2015), Biochim. Open, 1, 28-39 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Escherichia coli

Protein Variants

Protein Variants Comment Organism
A82P mutation in hinge region, 41% of wild-type activity. Unlike wild-type, the mutant unfolds by a non-two state mechanism in the presence of urea. Mutation affects both the shape and size of the protein significantly Escherichia coli
I65P mutation in hinge region, 6% of wild-type activity. Unlike wild-type, the mutant unfolds by a non-two state mechanism in the presence of urea. Mutation affects both the shape and size of the protein significantly Escherichia coli
V72P mutation in hinge region, 60% of wild-type activity. Unlike wild-type, the mutant unfolds by a non-two state mechanism in the presence of urea Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54500
-
gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A9L3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
RNAse T1 RNase T1 refolding assay Escherichia coli ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 23000, calculated from sequence Escherichia coli

Synonyms

Synonyms Comment Organism
FKBP22
-
Escherichia coli
FklB
-
Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
46
-
RNase T1 mutant I65P, pH not specified in the publication, temperature not specified in the publication Escherichia coli
338
-
RNase T1 mutant A82P, pH not specified in the publication, temperature not specified in the publication Escherichia coli
491
-
RNase T1 mutant V72P, pH not specified in the publication, temperature not specified in the publication Escherichia coli
818
-
RNase T1 wild-type, pH not specified in the publication, temperature not specified in the publication Escherichia coli