EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
1.1.1.203 | 0.04 |
- |
beta-D-galacturonate |
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer |
740959 |
1.1.1.203 | 0.07 |
- |
beta-D-galacturonate |
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer |
740959 |
1.1.1.203 | 41 |
- |
beta-D-galacturonate |
mutant enzyme I114T, at pH 8.0 and 25°C |
762310 |
1.1.1.203 | 78 |
- |
beta-D-galacturonate |
mutant enzyme Q55R/I88L/N99K/L100H/K102V/P103K/I105V/T113V/I114T/Y116F/L158A/N159C/I160V/A172V/M175I/D246N, at pH 8.0 and 25°C |
762310 |
1.1.1.203 | 88 |
- |
beta-D-galacturonate |
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer |
740959 |
1.1.1.203 | 96 |
- |
beta-D-galacturonate |
pH 8.0, 25°C |
740393 |
1.1.1.203 | 98 |
- |
beta-D-galacturonate |
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer |
740959 |
1.1.1.203 | 104 |
- |
beta-D-galacturonate |
mutant enzyme T113V/A172V/M175I, at pH 8.0 and 25°C |
762310 |
1.1.1.203 | 111 |
- |
beta-D-galacturonate |
mutant enzyme Y116F, at pH 8.0 and 25°C |
762310 |
1.1.1.203 | 112 |
- |
beta-D-galacturonate |
mutant enzyme I88L, at pH 8.0 and 25°C |
762310 |