Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Chromohalobacter salexigens |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 0.1 M MES pH6.5 and 30% (w/v) 2-methyl-2-4-pentanediol | Chromohalobacter salexigens |
Protein Variants | Comment | Organism |
---|---|---|
A172V | the mutant shows reduced catalytic efficiency with beta-D-glucuronate and beta-D-galacturonate compared to the wild type enzyme | Chromohalobacter salexigens |
D246N | the mutant shows reduced catalytic efficiency with beta-D-glucuronate and beta-D-galacturonate compared to the wild type enzyme | Chromohalobacter salexigens |
I114T | the mutant shows reduced catalytic efficiency with beta-D-glucuronate and beta-D-galacturonate compared to the wild type enzyme | Chromohalobacter salexigens |
I88L | the mutant shows reduced catalytic efficiency with beta-D-glucuronate and beta-D-galacturonate compared to the wild type enzyme | Chromohalobacter salexigens |
L158A/N159C/I160V | the mutant shows increased catalytic efficiency with beta-D-glucuronate and beta-D-galacturonate compared to the wild type enzyme | Chromohalobacter salexigens |
M175I | the mutant shows reduced catalytic efficiency with beta-D-glucuronate and beta-D-galacturonate compared to the wild type enzyme | Chromohalobacter salexigens |
N99K/L100H/K102V/P103K/I105V | the mutant shows wild type activity with beta-D-glucuronate and reduced catalytic efficiency with beta-D-galacturonate compared to the wild type enzyme | Chromohalobacter salexigens |
N99K/L100H/K102V/P103K/I105V/Y116F/T113V/A172V/M175I/D236N | the mutant shows reduced catalytic efficiency with beta-D-glucuronate and beta-D-galacturonate compared to the wild type enzyme | Chromohalobacter salexigens |
Q55R | the mutant shows wild type activity with beta-D-glucuronate and reduced catalytic efficiency with beta-D-galacturonate compared to the wild type enzyme | Chromohalobacter salexigens |
Q55R/I88L/N99K/L100H/K102V/P103K/I105V/T113V/I114T/Y116F/L158A/N159C/I160V/A172V/M175I/D246N | the mutant shows reduced catalytic efficiency with beta-D-glucuronate and beta-D-galacturonate compared to the wild type enzyme | Chromohalobacter salexigens |
T113V | the mutant shows reduced catalytic efficiency with beta-D-glucuronate and beta-D-galacturonate compared to the wild type enzyme | Chromohalobacter salexigens |
T113V/A172V/D246N | the mutant shows reduced catalytic efficiency with beta-D-glucuronate and beta-D-galacturonate compared to the wild type enzyme | Chromohalobacter salexigens |
T113V/A172V/M175I | the mutant shows reduced catalytic efficiency with beta-D-glucuronate and beta-D-galacturonate compared to the wild type enzyme | Chromohalobacter salexigens |
T113V/A172V/M175I/D236N | the mutant shows reduced catalytic efficiency with beta-D-glucuronate and beta-D-galacturonate compared to the wild type enzyme | Chromohalobacter salexigens |
T113V/M175I/D246N | the mutant shows increased catalytic efficiency with beta-D-glucuronate and decreased catalytic efficiency with beta-D-galacturonate compared to the wild type enzyme | Chromohalobacter salexigens |
Y116F | the mutant shows reduced catalytic efficiency with beta-D-glucuronate and beta-D-galacturonate compared to the wild type enzyme | Chromohalobacter salexigens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
beta-D-galacturonate | mutant enzyme N99K/L100H/K102V/P103K/I105V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
0.14 | - |
beta-D-galacturonate | mutant enzyme L158A/N159C/I160V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
0.15 | - |
beta-D-galacturonate | mutant enzyme T113V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
0.16 | - |
beta-D-galacturonate | mutant enzyme N99K/L100H/K102V/P103K/I105V/Y116F/T113V/A172V/M175I/D236N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
0.18 | - |
beta-D-galacturonate | wild type enzyme, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
0.18 | - |
beta-D-galacturonate | mutant enzyme A172V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
0.18 | - |
beta-D-galacturonate | mutant enzyme D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
0.18 | - |
beta-D-galacturonate | mutant enzyme M175I, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
0.18 | - |
beta-D-galacturonate | mutant enzyme T113V/A172V/M175I, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
0.18 | - |
beta-D-galacturonate | mutant enzyme T113V/A172V/M175I/D236N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
0.19 | - |
beta-D-galacturonate | mutant enzyme Q55R, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
0.19 | - |
beta-D-galacturonate | mutant enzyme T113V/M175I/D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
0.2 | - |
beta-D-galacturonate | mutant enzyme I88L, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
0.21 | - |
beta-D-galacturonate | mutant enzyme Q55R/I88L/N99K/L100H/K102V/P103K/I105V/T113V/I114T/Y116F/L158A/N159C/I160V/A172V/M175I/D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
0.22 | - |
beta-D-galacturonate | mutant enzyme T113V/A172V/D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
0.22 | - |
beta-D-galacturonate | mutant enzyme Y116F, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
0.41 | - |
beta-D-galacturonate | mutant enzyme I114T, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
0.43 | - |
beta-D-glucuronate | mutant enzyme N99K/L100H/K102V/P103K/I105V/Y116F/T113V/A172V/M175I/D236N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
0.65 | - |
beta-D-glucuronate | mutant enzyme Q55R/I88L/N99K/L100H/K102V/P103K/I105V/T113V/I114T/Y116F/L158A/N159C/I160V/A172V/M175I/D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
0.9 | - |
beta-D-glucuronate | mutant enzyme N99K/L100H/K102V/P103K/I105V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
0.95 | - |
beta-D-glucuronate | mutant enzyme T113V/A172V/M175I/D236N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
1.03 | - |
beta-D-glucuronate | mutant enzyme M175I, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
1.1 | - |
beta-D-glucuronate | mutant enzyme T113V/A172V/M175I, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
1.1 | - |
beta-D-glucuronate | mutant enzyme T113V/M175I/D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
1.2 | - |
beta-D-glucuronate | mutant enzyme L158A/N159C/I160V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
1.3 | - |
beta-D-glucuronate | mutant enzyme D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
1.5 | - |
beta-D-glucuronate | mutant enzyme I88L, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
1.5 | - |
beta-D-glucuronate | mutant enzyme T113V/A172V/D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
1.6 | - |
beta-D-glucuronate | wild type enzyme, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
1.7 | - |
beta-D-glucuronate | mutant enzyme A172V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
1.7 | - |
beta-D-glucuronate | mutant enzyme Q55R, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
1.7 | - |
beta-D-glucuronate | mutant enzyme T113V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
2 | - |
beta-D-glucuronate | mutant enzyme Y116F, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
3.2 | - |
beta-D-glucuronate | mutant enzyme I114T, at pH 8.0 and 25°C | Chromohalobacter salexigens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-galacturonate + NAD+ | Chromohalobacter salexigens | - |
D-galactaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-glucuronate + NAD+ | Chromohalobacter salexigens | - |
D-glucaro-1,5-lactone + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chromohalobacter salexigens | Q1QUN7 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex 200 gel filtration | Chromohalobacter salexigens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-galacturonate + NAD+ | - |
Chromohalobacter salexigens | D-galactaro-1,5-lactone + NADH + H+ | - |
? | |
beta-D-glucuronate + NAD+ | - |
Chromohalobacter salexigens | D-glucaro-1,5-lactone + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Udh | - |
Chromohalobacter salexigens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | 8 | beta-D-galacturonate | mutant enzyme Q55R, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
17 | - |
beta-D-galacturonate | mutant enzyme I114T, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
17 | - |
beta-D-galacturonate | mutant enzyme N99K/L100H/K102V/P103K/I105V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
17 | - |
beta-D-galacturonate | mutant enzyme Q55R/I88L/N99K/L100H/K102V/P103K/I105V/T113V/I114T/Y116F/L158A/N159C/I160V/A172V/M175I/D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
19 | - |
beta-D-galacturonate | mutant enzyme T113V/A172V/M175I, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
20 | - |
beta-D-galacturonate | mutant enzyme M175I, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
20 | - |
beta-D-galacturonate | mutant enzyme N99K/L100H/K102V/P103K/I105V/Y116F/T113V/A172V/M175I/D236N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
20 | - |
beta-D-galacturonate | mutant enzyme T113V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
22 | - |
beta-D-galacturonate | mutant enzyme D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
22 | - |
beta-D-galacturonate | mutant enzyme I88L, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
22 | - |
beta-D-galacturonate | mutant enzyme T113V/A172V/M175I/D236N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
25 | - |
beta-D-galacturonate | mutant enzyme Y116F, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
27 | - |
beta-D-galacturonate | mutant enzyme A172V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
27 | - |
beta-D-galacturonate | mutant enzyme L158A/N159C/I160V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
29 | - |
beta-D-galacturonate | mutant enzyme T113V/M175I/D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
30 | - |
beta-D-galacturonate | mutant enzyme T113V/A172V/D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
30.8 | - |
beta-D-galacturonate | wild type enzyme, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
50 | - |
beta-D-glucuronate | mutant enzyme Q55R/I88L/N99K/L100H/K102V/P103K/I105V/T113V/I114T/Y116F/L158A/N159C/I160V/A172V/M175I/D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
59 | - |
beta-D-glucuronate | mutant enzyme N99K/L100H/K102V/P103K/I105V/Y116F/T113V/A172V/M175I/D236N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
119 | - |
beta-D-glucuronate | mutant enzyme T113V/A172V/M175I, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
130 | - |
beta-D-glucuronate | mutant enzyme T113V/A172V/M175I/D236N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
142 | - |
beta-D-glucuronate | mutant enzyme N99K/L100H/K102V/P103K/I105V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
153 | - |
beta-D-glucuronate | mutant enzyme M175I, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
155 | - |
beta-D-glucuronate | mutant enzyme I114T, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
179 | - |
beta-D-glucuronate | mutant enzyme T113V/M175I/D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
192 | - |
beta-D-glucuronate | mutant enzyme D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
192 | - |
beta-D-glucuronate | mutant enzyme I88L, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
192 | - |
beta-D-glucuronate | mutant enzyme T113V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
200 | - |
beta-D-glucuronate | mutant enzyme Y116F, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
214 | - |
beta-D-glucuronate | mutant enzyme T113V/A172V/D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
219 | - |
beta-D-glucuronate | mutant enzyme L158A/N159C/I160V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
225 | - |
beta-D-glucuronate | mutant enzyme A172V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
263 | - |
beta-D-glucuronate | wild type enzyme, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
270 | - |
beta-D-glucuronate | mutant enzyme Q55R, at pH 8.0 and 25°C | Chromohalobacter salexigens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Chromohalobacter salexigens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
41 | - |
beta-D-galacturonate | mutant enzyme I114T, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
48 | - |
beta-D-glucuronate | mutant enzyme I114T, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
77 | - |
beta-D-glucuronate | mutant enzyme Q55R/I88L/N99K/L100H/K102V/P103K/I105V/T113V/I114T/Y116F/L158A/N159C/I160V/A172V/M175I/D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
78 | - |
beta-D-galacturonate | mutant enzyme Q55R/I88L/N99K/L100H/K102V/P103K/I105V/T113V/I114T/Y116F/L158A/N159C/I160V/A172V/M175I/D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
101 | - |
beta-D-glucuronate | mutant enzyme Y116F, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
104 | - |
beta-D-galacturonate | mutant enzyme T113V/A172V/M175I, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
111 | - |
beta-D-glucuronate | mutant enzyme T113V/A172V/M175I, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
111 | - |
beta-D-galacturonate | mutant enzyme Y116F, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
112 | - |
beta-D-galacturonate | mutant enzyme I88L, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
115 | - |
beta-D-glucuronate | mutant enzyme T113V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
116 | - |
beta-D-galacturonate | mutant enzyme M175I, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
125 | - |
beta-D-glucuronate | mutant enzyme I88L, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
125 | - |
beta-D-galacturonate | mutant enzyme N99K/L100H/K102V/P103K/I105V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
125 | - |
beta-D-galacturonate | mutant enzyme T113V/A172V/M175I/D236N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
126 | - |
beta-D-galacturonate | mutant enzyme D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
126 | - |
beta-D-galacturonate | mutant enzyme N99K/L100H/K102V/P103K/I105V/Y116F/T113V/A172V/M175I/D236N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
132 | - |
beta-D-galacturonate | mutant enzyme T113V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
136 | - |
beta-D-glucuronate | mutant enzyme A172V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
136 | - |
beta-D-glucuronate | mutant enzyme N99K/L100H/K102V/P103K/I105V/Y116F/T113V/A172V/M175I/D236N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
138 | - |
beta-D-galacturonate | mutant enzyme T113V/A172V/D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
138 | - |
beta-D-glucuronate | mutant enzyme T113V/A172V/M175I/D236N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
139 | - |
beta-D-glucuronate | mutant enzyme T113V/A172V/D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
146 | - |
beta-D-glucuronate | mutant enzyme D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
149 | - |
beta-D-glucuronate | mutant enzyme M175I, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
151 | - |
beta-D-galacturonate | mutant enzyme A172V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
152 | - |
beta-D-galacturonate | mutant enzyme Q55R, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
155 | - |
beta-D-galacturonate | mutant enzyme T113V/M175I/D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
161 | - |
beta-D-glucuronate | wild type enzyme, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
161 | - |
beta-D-glucuronate | mutant enzyme Q55R, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
162 | - |
beta-D-glucuronate | mutant enzyme N99K/L100H/K102V/P103K/I105V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
164 | - |
beta-D-glucuronate | mutant enzyme T113V/M175I/D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
172 | - |
beta-D-galacturonate | wild type enzyme, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
186 | - |
beta-D-glucuronate | mutant enzyme L158A/N159C/I160V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
187 | - |
beta-D-galacturonate | mutant enzyme L158A/N159C/I160V, at pH 8.0 and 25°C | Chromohalobacter salexigens |