BRENDA - Enzyme Database show
show all sequences of 1.1.1.203

Characterization of uronate dehydrogenases catalysing the initial step in an oxidative pathway

Pick, A.; Schmid, J.; Sieber, V.; Microb. Biotechnol. 8, 633-643 (2015)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene udh, phylogenetic tree, recombinant soluble expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
Agrobacterium tumefaciens
gene udh, phylogenetic tree, recombinant soluble expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
Fulvimarina pelagi
gene udh, phylogenetic tree, recombinant soluble expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
Oceanicola granulosus
gene udh, phylogenetic tree, recombinant soluble expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
Pseudomonas syringae pv. tomato
gene udh, phylogenetic tree, recombinant soluble expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
Streptomyces viridochromogenes
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Michaelis-Menten kinetics
Agrobacterium tumefaciens
additional information
-
additional information
Michaelis-Menten kinetics
Fulvimarina pelagi
additional information
-
additional information
Michaelis-Menten kinetics
Oceanicola granulosus
additional information
-
additional information
Michaelis-Menten kinetics
Pseudomonas syringae pv. tomato
additional information
-
additional information
Michaelis-Menten kinetics
Streptomyces viridochromogenes
0.02
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Fulvimarina pelagi
0.03
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Fulvimarina pelagi
0.03
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Pseudomonas syringae pv. tomato
0.04
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Pseudomonas syringae pv. tomato
0.1
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Agrobacterium tumefaciens
0.1
-
NAD+
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Agrobacterium tumefaciens
0.1
-
NAD+
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Streptomyces viridochromogenes
0.14
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Pseudomonas syringae pv. tomato
0.2
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Streptomyces viridochromogenes
0.2
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Agrobacterium tumefaciens
0.2
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Fulvimarina pelagi
0.2
-
NAD+
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Fulvimarina pelagi
0.2
-
NAD+
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Pseudomonas syringae pv. tomato
0.3
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Agrobacterium tumefaciens
0.3
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Fulvimarina pelagi
0.3
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Streptomyces viridochromogenes
0.4
-
NAD+
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Oceanicola granulosus
0.5
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Pseudomonas syringae pv. tomato
1
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Agrobacterium tumefaciens
6.4
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Fulvimarina pelagi
7.3
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Pseudomonas syringae pv. tomato
8.3
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Agrobacterium tumefaciens
9
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Streptomyces viridochromogenes
9.5
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Fulvimarina pelagi
13.6
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Streptomyces viridochromogenes
19.4
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Agrobacterium tumefaciens
22.05
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Pseudomonas syringae pv. tomato
55
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Oceanicola granulosus
59
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Oceanicola granulosus
64
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Oceanicola granulosus
64
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Oceanicola granulosus
112
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Oceanicola granulosus
142
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Oceanicola granulosus
465
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Streptomyces viridochromogenes
628
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Streptomyces viridochromogenes
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
beta-D-galacturonate + NAD+
Streptomyces viridochromogenes
-
D-galactaro-1,5-lactone + NADH + H+
-
-
?
beta-D-galacturonate + NAD+
Pseudomonas syringae pv. tomato
-
D-galactaro-1,5-lactone + NADH + H+
-
-
?
beta-D-galacturonate + NAD+
Agrobacterium tumefaciens
-
D-galactaro-1,5-lactone + NADH + H+
-
-
?
beta-D-galacturonate + NAD+
Fulvimarina pelagi
-
D-galactaro-1,5-lactone + NADH + H+
-
-
?
beta-D-galacturonate + NAD+
Oceanicola granulosus
-
D-galactaro-1,5-lactone + NADH + H+
-
-
?
beta-D-galacturonate + NAD+
Oceanicola granulosus DSM 15982
-
D-galactaro-1,5-lactone + NADH + H+
-
-
?
beta-D-galacturonate + NAD+
Streptomyces viridochromogenes DSM 40736
-
D-galactaro-1,5-lactone + NADH + H+
-
-
?
beta-D-galacturonate + NAD+
Pseudomonas syringae pv. tomato DC3000
-
D-galactaro-1,5-lactone + NADH + H+
-
-
?
beta-D-galacturonate + NAD+
Agrobacterium tumefaciens C58 / ATCC 33970
-
D-galactaro-1,5-lactone + NADH + H+
-
-
?
beta-D-glucuronate + NAD+
Streptomyces viridochromogenes
-
D-glucaro-1,5-lactone + NADH + H+
-
-
?
beta-D-glucuronate + NAD+
Pseudomonas syringae pv. tomato
-
D-glucaro-1,5-lactone + NADH + H+
-
-
?
beta-D-glucuronate + NAD+
Agrobacterium tumefaciens
-
D-glucaro-1,5-lactone + NADH + H+
-
-
?
beta-D-glucuronate + NAD+
Fulvimarina pelagi
-
D-glucaro-1,5-lactone + NADH + H+
-
-
?
beta-D-glucuronate + NAD+
Oceanicola granulosus
-
D-glucaro-1,5-lactone + NADH + H+
-
-
?
beta-D-glucuronate + NAD+
Oceanicola granulosus DSM 15982
-
D-glucaro-1,5-lactone + NADH + H+
-
-
?
beta-D-glucuronate + NAD+
Streptomyces viridochromogenes DSM 40736
-
D-glucaro-1,5-lactone + NADH + H+
-
-
?
beta-D-glucuronate + NAD+
Pseudomonas syringae pv. tomato DC3000
-
D-glucaro-1,5-lactone + NADH + H+
-
-
?
beta-D-glucuronate + NAD+
Agrobacterium tumefaciens C58 / ATCC 33970
-
D-glucaro-1,5-lactone + NADH + H+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Fulvimarina pelagi
-
-
-
Oceanicola granulosus
-
-
-
Oceanicola granulosus DSM 15982
-
-
-
Streptomyces viridochromogenes
-
-
-
Streptomyces viridochromogenes DSM 40736
-
-
-
Agrobacterium tumefaciens
Q7CRQ0
-
-
Agrobacterium tumefaciens C58 / ATCC 33970
Q7CRQ0
-
-
Pseudomonas syringae pv. tomato
Q888H1
-
-
Pseudomonas syringae pv. tomato DC3000
Q888H1
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-D-galacturonate + NAD+
-
740959
Streptomyces viridochromogenes
D-galactaro-1,5-lactone + NADH + H+
-
-
-
?
beta-D-galacturonate + NAD+
-
740959
Pseudomonas syringae pv. tomato
D-galactaro-1,5-lactone + NADH + H+
-
-
-
?
beta-D-galacturonate + NAD+
-
740959
Agrobacterium tumefaciens
D-galactaro-1,5-lactone + NADH + H+
-
-
-
?
beta-D-galacturonate + NAD+
-
740959
Fulvimarina pelagi
D-galactaro-1,5-lactone + NADH + H+
-
-
-
?
beta-D-galacturonate + NAD+
-
740959
Oceanicola granulosus
D-galactaro-1,5-lactone + NADH + H+
-
-
-
?
beta-D-galacturonate + NAD+
-
740959
Oceanicola granulosus DSM 15982
D-galactaro-1,5-lactone + NADH + H+
-
-
-
?
beta-D-galacturonate + NAD+
-
740959
Streptomyces viridochromogenes DSM 40736
D-galactaro-1,5-lactone + NADH + H+
-
-
-
?
beta-D-galacturonate + NAD+
-
740959
Pseudomonas syringae pv. tomato DC3000
D-galactaro-1,5-lactone + NADH + H+
-
-
-
?
beta-D-galacturonate + NAD+
-
740959
Agrobacterium tumefaciens C58 / ATCC 33970
D-galactaro-1,5-lactone + NADH + H+
-
-
-
?
beta-D-glucuronate + NAD+
-
740959
Streptomyces viridochromogenes
D-glucaro-1,5-lactone + NADH + H+
-
-
-
?
beta-D-glucuronate + NAD+
-
740959
Pseudomonas syringae pv. tomato
D-glucaro-1,5-lactone + NADH + H+
-
-
-
?
beta-D-glucuronate + NAD+
-
740959
Agrobacterium tumefaciens
D-glucaro-1,5-lactone + NADH + H+
-
-
-
?
beta-D-glucuronate + NAD+
-
740959
Fulvimarina pelagi
D-glucaro-1,5-lactone + NADH + H+
-
-
-
?
beta-D-glucuronate + NAD+
-
740959
Oceanicola granulosus
D-glucaro-1,5-lactone + NADH + H+
-
-
-
?
beta-D-glucuronate + NAD+
-
740959
Oceanicola granulosus DSM 15982
D-glucaro-1,5-lactone + NADH + H+
-
-
-
?
beta-D-glucuronate + NAD+
-
740959
Streptomyces viridochromogenes DSM 40736
D-glucaro-1,5-lactone + NADH + H+
-
-
-
?
beta-D-glucuronate + NAD+
-
740959
Pseudomonas syringae pv. tomato DC3000
D-glucaro-1,5-lactone + NADH + H+
-
-
-
?
beta-D-glucuronate + NAD+
-
740959
Agrobacterium tumefaciens C58 / ATCC 33970
D-glucaro-1,5-lactone + NADH + H+
-
-
-
?
D-mannuronate + NAD+
-
740959
Streptomyces viridochromogenes
? + NADH + H+
-
-
-
?
D-mannuronate + NAD+
-
740959
Pseudomonas syringae pv. tomato
? + NADH + H+
-
-
-
?
D-mannuronate + NAD+
-
740959
Agrobacterium tumefaciens
? + NADH + H+
-
-
-
?
D-mannuronate + NAD+
-
740959
Fulvimarina pelagi
? + NADH + H+
-
-
-
?
D-mannuronate + NAD+
-
740959
Oceanicola granulosus
? + NADH + H+
-
-
-
?
D-mannuronate + NAD+
-
740959
Oceanicola granulosus DSM 15982
? + NADH + H+
-
-
-
?
D-mannuronate + NAD+
-
740959
Streptomyces viridochromogenes DSM 40736
? + NADH + H+
-
-
-
?
D-mannuronate + NAD+
-
740959
Pseudomonas syringae pv. tomato DC3000
? + NADH + H+
-
-
-
?
D-mannuronate + NAD+
-
740959
Agrobacterium tumefaciens C58 / ATCC 33970
? + NADH + H+
-
-
-
?
additional information
substrate and cofactor specificity of Udhs, overview
740959
Streptomyces viridochromogenes
?
-
-
-
-
additional information
substrate and cofactor specificity of Udhs, overview
740959
Pseudomonas syringae pv. tomato
?
-
-
-
-
additional information
substrate and cofactor specificity of Udhs, overview
740959
Agrobacterium tumefaciens
?
-
-
-
-
additional information
substrate and cofactor specificity of Udhs, overview
740959
Fulvimarina pelagi
?
-
-
-
-
additional information
substrate and cofactor specificity of Udhs, overview
740959
Oceanicola granulosus
?
-
-
-
-
additional information
substrate and cofactor specificity of Udhs, overview
740959
Oceanicola granulosus DSM 15982
?
-
-
-
-
additional information
substrate and cofactor specificity of Udhs, overview
740959
Streptomyces viridochromogenes DSM 40736
?
-
-
-
-
additional information
substrate and cofactor specificity of Udhs, overview
740959
Pseudomonas syringae pv. tomato DC3000
?
-
-
-
-
additional information
substrate and cofactor specificity of Udhs, overview
740959
Agrobacterium tumefaciens C58 / ATCC 33970
?
-
-
-
-
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
-
Oceanicola granulosus
35
-
-
Pseudomonas syringae pv. tomato
45
-
-
Streptomyces viridochromogenes
60
-
-
Agrobacterium tumefaciens
60
-
-
Fulvimarina pelagi
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
15
50
activity range, inactivation at 55C, profile overview
Pseudomonas syringae pv. tomato
15
55
activity range, profile overview
Streptomyces viridochromogenes
15
60
activity is constantly increasing within this range, profile overview
Agrobacterium tumefaciens
15
60
activity is constantly increasing within this range, profile overview
Fulvimarina pelagi
15
60
activity range, profile overview
Oceanicola granulosus
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
37
-
purified recombinant His-tagged enzyme, 10% activity remaning after 100 min, inactivation within 150 min
Agrobacterium tumefaciens
37
-
purified recombinant His-tagged enzyme, 50% activity remaining after 120 min, 25% activity remaining after 300 min
Fulvimarina pelagi
37
-
purified recombinant His-tagged enzyme, 50% remaining activity after 1000-1500 min, 30% remaning after 2500 min
Oceanicola granulosus
37
-
purified recombinant His-tagged enzyme, inactivation within 50 min
Pseudomonas syringae pv. tomato
37
-
purified recombinant His-tagged enzyme, inactivation within 50 min
Streptomyces viridochromogenes
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Oceanicola granulosus
3.4
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Oceanicola granulosus
4
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Oceanicola granulosus
4
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Oceanicola granulosus
6
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Oceanicola granulosus
6.4
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Pseudomonas syringae pv. tomato
7
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Oceanicola granulosus
7
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Pseudomonas syringae pv. tomato
7
-
NAD+
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Oceanicola granulosus
8
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Streptomyces viridochromogenes
9
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Streptomyces viridochromogenes
10
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Fulvimarina pelagi
13
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Fulvimarina pelagi
17
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Streptomyces viridochromogenes
21
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Pseudomonas syringae pv. tomato
23
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Streptomyces viridochromogenes
29
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Pseudomonas syringae pv. tomato
53
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Streptomyces viridochromogenes
58
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Fulvimarina pelagi
59
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Agrobacterium tumefaciens
62
-
NAD+
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Streptomyces viridochromogenes
63
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Pseudomonas syringae pv. tomato
75
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Streptomyces viridochromogenes
76
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Agrobacterium tumefaciens
78
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Fulvimarina pelagi
82
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Fulvimarina pelagi
85
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Agrobacterium tumefaciens
92
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Agrobacterium tumefaciens
107
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Fulvimarina pelagi
108
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Pseudomonas syringae pv. tomato
113
-
NAD+
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Fulvimarina pelagi
144
-
NAD+
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Agrobacterium tumefaciens
171
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Agrobacterium tumefaciens
215
-
NAD+
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Pseudomonas syringae pv. tomato
333
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Agrobacterium tumefaciens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Oceanicola granulosus
8
9
-
Agrobacterium tumefaciens
8
-
-
Pseudomonas syringae pv. tomato
9
-
-
Fulvimarina pelagi
9.5
10
-
Streptomyces viridochromogenes
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5
10
activity range, profile overview
Agrobacterium tumefaciens
5
10
activity range, profile overview
Fulvimarina pelagi
5
10
activity is constantly increasing in this range, profile overview
Streptomyces viridochromogenes
5.5
9.5
activity range, profile overview
Oceanicola granulosus
5.5
9.5
activity range, profile overview
Pseudomonas syringae pv. tomato
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Agrobacterium tumefaciens
NAD+
-
Fulvimarina pelagi
NAD+
-
Oceanicola granulosus
NAD+
-
Pseudomonas syringae pv. tomato
NAD+
-
Streptomyces viridochromogenes
NADH
-
Agrobacterium tumefaciens
NADH
-
Fulvimarina pelagi
NADH
-
Oceanicola granulosus
NADH
-
Pseudomonas syringae pv. tomato
NADH
-
Streptomyces viridochromogenes
Cloned(Commentary) (protein specific)
Commentary
Organism
gene udh, phylogenetic tree, recombinant soluble expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
Agrobacterium tumefaciens
gene udh, phylogenetic tree, recombinant soluble expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
Fulvimarina pelagi
gene udh, phylogenetic tree, recombinant soluble expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
Oceanicola granulosus
gene udh, phylogenetic tree, recombinant soluble expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
Pseudomonas syringae pv. tomato
gene udh, phylogenetic tree, recombinant soluble expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
Streptomyces viridochromogenes
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Agrobacterium tumefaciens
NAD+
-
Fulvimarina pelagi
NAD+
-
Oceanicola granulosus
NAD+
-
Pseudomonas syringae pv. tomato
NAD+
-
Streptomyces viridochromogenes
NADH
-
Agrobacterium tumefaciens
NADH
-
Fulvimarina pelagi
NADH
-
Oceanicola granulosus
NADH
-
Pseudomonas syringae pv. tomato
NADH
-
Streptomyces viridochromogenes
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Michaelis-Menten kinetics
Agrobacterium tumefaciens
additional information
-
additional information
Michaelis-Menten kinetics
Fulvimarina pelagi
additional information
-
additional information
Michaelis-Menten kinetics
Oceanicola granulosus
additional information
-
additional information
Michaelis-Menten kinetics
Pseudomonas syringae pv. tomato
additional information
-
additional information
Michaelis-Menten kinetics
Streptomyces viridochromogenes
0.02
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Fulvimarina pelagi
0.03
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Fulvimarina pelagi
0.03
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Pseudomonas syringae pv. tomato
0.04
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Pseudomonas syringae pv. tomato
0.1
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Agrobacterium tumefaciens
0.1
-
NAD+
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Agrobacterium tumefaciens
0.1
-
NAD+
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Streptomyces viridochromogenes
0.14
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Pseudomonas syringae pv. tomato
0.2
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Streptomyces viridochromogenes
0.2
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Agrobacterium tumefaciens
0.2
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Fulvimarina pelagi
0.2
-
NAD+
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Fulvimarina pelagi
0.2
-
NAD+
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Pseudomonas syringae pv. tomato
0.3
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Agrobacterium tumefaciens
0.3
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Fulvimarina pelagi
0.3
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Streptomyces viridochromogenes
0.4
-
NAD+
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Oceanicola granulosus
0.5
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Pseudomonas syringae pv. tomato
1
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Agrobacterium tumefaciens
6.4
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Fulvimarina pelagi
7.3
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Pseudomonas syringae pv. tomato
8.3
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Agrobacterium tumefaciens
9
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Streptomyces viridochromogenes
9.5
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Fulvimarina pelagi
13.6
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Streptomyces viridochromogenes
19.4
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Agrobacterium tumefaciens
22.05
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Pseudomonas syringae pv. tomato
55
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Oceanicola granulosus
59
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Oceanicola granulosus
64
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Oceanicola granulosus
64
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Oceanicola granulosus
112
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Oceanicola granulosus
142
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Oceanicola granulosus
465
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Streptomyces viridochromogenes
628
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Streptomyces viridochromogenes
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
beta-D-galacturonate + NAD+
Streptomyces viridochromogenes
-
D-galactaro-1,5-lactone + NADH + H+
-
-
?
beta-D-galacturonate + NAD+
Pseudomonas syringae pv. tomato
-
D-galactaro-1,5-lactone + NADH + H+
-
-
?
beta-D-galacturonate + NAD+
Agrobacterium tumefaciens
-
D-galactaro-1,5-lactone + NADH + H+
-
-
?
beta-D-galacturonate + NAD+
Fulvimarina pelagi
-
D-galactaro-1,5-lactone + NADH + H+
-
-
?
beta-D-galacturonate + NAD+
Oceanicola granulosus
-
D-galactaro-1,5-lactone + NADH + H+
-
-
?
beta-D-galacturonate + NAD+
Oceanicola granulosus DSM 15982
-
D-galactaro-1,5-lactone + NADH + H+
-
-
?
beta-D-galacturonate + NAD+
Streptomyces viridochromogenes DSM 40736
-
D-galactaro-1,5-lactone + NADH + H+
-
-
?
beta-D-galacturonate + NAD+
Pseudomonas syringae pv. tomato DC3000
-
D-galactaro-1,5-lactone + NADH + H+
-
-
?
beta-D-galacturonate + NAD+
Agrobacterium tumefaciens C58 / ATCC 33970
-
D-galactaro-1,5-lactone + NADH + H+
-
-
?
beta-D-glucuronate + NAD+
Streptomyces viridochromogenes
-
D-glucaro-1,5-lactone + NADH + H+
-
-
?
beta-D-glucuronate + NAD+
Pseudomonas syringae pv. tomato
-
D-glucaro-1,5-lactone + NADH + H+
-
-
?
beta-D-glucuronate + NAD+
Agrobacterium tumefaciens
-
D-glucaro-1,5-lactone + NADH + H+
-
-
?
beta-D-glucuronate + NAD+
Fulvimarina pelagi
-
D-glucaro-1,5-lactone + NADH + H+
-
-
?
beta-D-glucuronate + NAD+
Oceanicola granulosus
-
D-glucaro-1,5-lactone + NADH + H+
-
-
?
beta-D-glucuronate + NAD+
Oceanicola granulosus DSM 15982
-
D-glucaro-1,5-lactone + NADH + H+
-
-
?
beta-D-glucuronate + NAD+
Streptomyces viridochromogenes DSM 40736
-
D-glucaro-1,5-lactone + NADH + H+
-
-
?
beta-D-glucuronate + NAD+
Pseudomonas syringae pv. tomato DC3000
-
D-glucaro-1,5-lactone + NADH + H+
-
-
?
beta-D-glucuronate + NAD+
Agrobacterium tumefaciens C58 / ATCC 33970
-
D-glucaro-1,5-lactone + NADH + H+
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-D-galacturonate + NAD+
-
740959
Streptomyces viridochromogenes
D-galactaro-1,5-lactone + NADH + H+
-
-
-
?
beta-D-galacturonate + NAD+
-
740959
Pseudomonas syringae pv. tomato
D-galactaro-1,5-lactone + NADH + H+
-
-
-
?
beta-D-galacturonate + NAD+
-
740959
Agrobacterium tumefaciens
D-galactaro-1,5-lactone + NADH + H+
-
-
-
?
beta-D-galacturonate + NAD+
-
740959
Fulvimarina pelagi
D-galactaro-1,5-lactone + NADH + H+
-
-
-
?
beta-D-galacturonate + NAD+
-
740959
Oceanicola granulosus
D-galactaro-1,5-lactone + NADH + H+
-
-
-
?
beta-D-galacturonate + NAD+
-
740959
Oceanicola granulosus DSM 15982
D-galactaro-1,5-lactone + NADH + H+
-
-
-
?
beta-D-galacturonate + NAD+
-
740959
Streptomyces viridochromogenes DSM 40736
D-galactaro-1,5-lactone + NADH + H+
-
-
-
?
beta-D-galacturonate + NAD+
-
740959
Pseudomonas syringae pv. tomato DC3000
D-galactaro-1,5-lactone + NADH + H+
-
-
-
?
beta-D-galacturonate + NAD+
-
740959
Agrobacterium tumefaciens C58 / ATCC 33970
D-galactaro-1,5-lactone + NADH + H+
-
-
-
?
beta-D-glucuronate + NAD+
-
740959
Streptomyces viridochromogenes
D-glucaro-1,5-lactone + NADH + H+
-
-
-
?
beta-D-glucuronate + NAD+
-
740959
Pseudomonas syringae pv. tomato
D-glucaro-1,5-lactone + NADH + H+
-
-
-
?
beta-D-glucuronate + NAD+
-
740959
Agrobacterium tumefaciens
D-glucaro-1,5-lactone + NADH + H+
-
-
-
?
beta-D-glucuronate + NAD+
-
740959
Fulvimarina pelagi
D-glucaro-1,5-lactone + NADH + H+
-
-
-
?
beta-D-glucuronate + NAD+
-
740959
Oceanicola granulosus
D-glucaro-1,5-lactone + NADH + H+
-
-
-
?
beta-D-glucuronate + NAD+
-
740959
Oceanicola granulosus DSM 15982
D-glucaro-1,5-lactone + NADH + H+
-
-
-
?
beta-D-glucuronate + NAD+
-
740959
Streptomyces viridochromogenes DSM 40736
D-glucaro-1,5-lactone + NADH + H+
-
-
-
?
beta-D-glucuronate + NAD+
-
740959
Pseudomonas syringae pv. tomato DC3000
D-glucaro-1,5-lactone + NADH + H+
-
-
-
?
beta-D-glucuronate + NAD+
-
740959
Agrobacterium tumefaciens C58 / ATCC 33970
D-glucaro-1,5-lactone + NADH + H+
-
-
-
?
D-mannuronate + NAD+
-
740959
Streptomyces viridochromogenes
? + NADH + H+
-
-
-
?
D-mannuronate + NAD+
-
740959
Pseudomonas syringae pv. tomato
? + NADH + H+
-
-
-
?
D-mannuronate + NAD+
-
740959
Agrobacterium tumefaciens
? + NADH + H+
-
-
-
?
D-mannuronate + NAD+
-
740959
Fulvimarina pelagi
? + NADH + H+
-
-
-
?
D-mannuronate + NAD+
-
740959
Oceanicola granulosus
? + NADH + H+
-
-
-
?
D-mannuronate + NAD+
-
740959
Oceanicola granulosus DSM 15982
? + NADH + H+
-
-
-
?
D-mannuronate + NAD+
-
740959
Streptomyces viridochromogenes DSM 40736
? + NADH + H+
-
-
-
?
D-mannuronate + NAD+
-
740959
Pseudomonas syringae pv. tomato DC3000
? + NADH + H+
-
-
-
?
D-mannuronate + NAD+
-
740959
Agrobacterium tumefaciens C58 / ATCC 33970
? + NADH + H+
-
-
-
?
additional information
substrate and cofactor specificity of Udhs, overview
740959
Streptomyces viridochromogenes
?
-
-
-
-
additional information
substrate and cofactor specificity of Udhs, overview
740959
Pseudomonas syringae pv. tomato
?
-
-
-
-
additional information
substrate and cofactor specificity of Udhs, overview
740959
Agrobacterium tumefaciens
?
-
-
-
-
additional information
substrate and cofactor specificity of Udhs, overview
740959
Fulvimarina pelagi
?
-
-
-
-
additional information
substrate and cofactor specificity of Udhs, overview
740959
Oceanicola granulosus
?
-
-
-
-
additional information
substrate and cofactor specificity of Udhs, overview
740959
Oceanicola granulosus DSM 15982
?
-
-
-
-
additional information
substrate and cofactor specificity of Udhs, overview
740959
Streptomyces viridochromogenes DSM 40736
?
-
-
-
-
additional information
substrate and cofactor specificity of Udhs, overview
740959
Pseudomonas syringae pv. tomato DC3000
?
-
-
-
-
additional information
substrate and cofactor specificity of Udhs, overview
740959
Agrobacterium tumefaciens C58 / ATCC 33970
?
-
-
-
-
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
-
Oceanicola granulosus
35
-
-
Pseudomonas syringae pv. tomato
45
-
-
Streptomyces viridochromogenes
60
-
-
Agrobacterium tumefaciens
60
-
-
Fulvimarina pelagi
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
15
50
activity range, inactivation at 55C, profile overview
Pseudomonas syringae pv. tomato
15
55
activity range, profile overview
Streptomyces viridochromogenes
15
60
activity is constantly increasing within this range, profile overview
Agrobacterium tumefaciens
15
60
activity is constantly increasing within this range, profile overview
Fulvimarina pelagi
15
60
activity range, profile overview
Oceanicola granulosus
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
37
-
purified recombinant His-tagged enzyme, 10% activity remaning after 100 min, inactivation within 150 min
Agrobacterium tumefaciens
37
-
purified recombinant His-tagged enzyme, 50% activity remaining after 120 min, 25% activity remaining after 300 min
Fulvimarina pelagi
37
-
purified recombinant His-tagged enzyme, 50% remaining activity after 1000-1500 min, 30% remaning after 2500 min
Oceanicola granulosus
37
-
purified recombinant His-tagged enzyme, inactivation within 50 min
Pseudomonas syringae pv. tomato
37
-
purified recombinant His-tagged enzyme, inactivation within 50 min
Streptomyces viridochromogenes
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Oceanicola granulosus
3.4
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Oceanicola granulosus
4
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Oceanicola granulosus
4
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Oceanicola granulosus
6
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Oceanicola granulosus
6.4
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Pseudomonas syringae pv. tomato
7
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Oceanicola granulosus
7
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Pseudomonas syringae pv. tomato
7
-
NAD+
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Oceanicola granulosus
8
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Streptomyces viridochromogenes
9
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Streptomyces viridochromogenes
10
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Fulvimarina pelagi
13
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Fulvimarina pelagi
17
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Streptomyces viridochromogenes
21
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Pseudomonas syringae pv. tomato
23
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Streptomyces viridochromogenes
29
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Pseudomonas syringae pv. tomato
53
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Streptomyces viridochromogenes
58
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Fulvimarina pelagi
59
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Agrobacterium tumefaciens
62
-
NAD+
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Streptomyces viridochromogenes
63
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Pseudomonas syringae pv. tomato
75
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Streptomyces viridochromogenes
76
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Agrobacterium tumefaciens
78
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Fulvimarina pelagi
82
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Fulvimarina pelagi
85
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Agrobacterium tumefaciens
92
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Agrobacterium tumefaciens
107
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Fulvimarina pelagi
108
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Pseudomonas syringae pv. tomato
113
-
NAD+
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Fulvimarina pelagi
144
-
NAD+
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Agrobacterium tumefaciens
171
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Agrobacterium tumefaciens
215
-
NAD+
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Pseudomonas syringae pv. tomato
333
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Agrobacterium tumefaciens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Oceanicola granulosus
8
9
-
Agrobacterium tumefaciens
8
-
-
Pseudomonas syringae pv. tomato
9
-
-
Fulvimarina pelagi
9.5
10
-
Streptomyces viridochromogenes
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5
10
activity range, profile overview
Agrobacterium tumefaciens
5
10
activity range, profile overview
Fulvimarina pelagi
5
10
activity is constantly increasing in this range, profile overview
Streptomyces viridochromogenes
5.5
9.5
activity range, profile overview
Oceanicola granulosus
5.5
9.5
activity range, profile overview
Pseudomonas syringae pv. tomato
General Information
General Information
Commentary
Organism
evolution
the enzyme belongs to the short-chain dehydrogenase/reductase (SDR) superfamily
Agrobacterium tumefaciens
evolution
the enzyme belongs to the short-chain dehydrogenase/reductase (SDR) superfamily
Fulvimarina pelagi
evolution
the enzyme belongs to the short-chain dehydrogenase/reductase (SDR) superfamily
Oceanicola granulosus
evolution
the enzyme belongs to the short-chain dehydrogenase/reductase (SDR) superfamily
Pseudomonas syringae pv. tomato
evolution
the enzyme belongs to the short-chain dehydrogenase/reductase (SDR) superfamily
Streptomyces viridochromogenes
metabolism
uronate dehydrogenases catalyse the initial step in an oxidative pathway
Agrobacterium tumefaciens
metabolism
uronate dehydrogenases catalyses the initial step in an oxidative pathway
Fulvimarina pelagi
metabolism
uronate dehydrogenases catalyse the initial step in an oxidative pathway
Oceanicola granulosus
metabolism
uronate dehydrogenases catalyse the initial step in an oxidative pathway
Pseudomonas syringae pv. tomato
metabolism
uronate dehydrogenases catalyse the initial step in an oxidative pathway
Streptomyces viridochromogenes
General Information (protein specific)
General Information
Commentary
Organism
evolution
the enzyme belongs to the short-chain dehydrogenase/reductase (SDR) superfamily
Agrobacterium tumefaciens
evolution
the enzyme belongs to the short-chain dehydrogenase/reductase (SDR) superfamily
Fulvimarina pelagi
evolution
the enzyme belongs to the short-chain dehydrogenase/reductase (SDR) superfamily
Oceanicola granulosus
evolution
the enzyme belongs to the short-chain dehydrogenase/reductase (SDR) superfamily
Pseudomonas syringae pv. tomato
evolution
the enzyme belongs to the short-chain dehydrogenase/reductase (SDR) superfamily
Streptomyces viridochromogenes
metabolism
uronate dehydrogenases catalyse the initial step in an oxidative pathway
Agrobacterium tumefaciens
metabolism
uronate dehydrogenases catalyses the initial step in an oxidative pathway
Fulvimarina pelagi
metabolism
uronate dehydrogenases catalyse the initial step in an oxidative pathway
Oceanicola granulosus
metabolism
uronate dehydrogenases catalyse the initial step in an oxidative pathway
Pseudomonas syringae pv. tomato
metabolism
uronate dehydrogenases catalyse the initial step in an oxidative pathway
Streptomyces viridochromogenes
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.03
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer; recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Oceanicola granulosus
0.04
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Oceanicola granulosus
0.07
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Oceanicola granulosus
0.1
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Oceanicola granulosus
0.11
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Oceanicola granulosus
0.3
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Pseudomonas syringae pv. tomato
0.7
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer; recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Streptomyces viridochromogenes
4
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Agrobacterium tumefaciens
9
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Pseudomonas syringae pv. tomato
11
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Agrobacterium tumefaciens
11
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Fulvimarina pelagi
12
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Fulvimarina pelagi
88
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Streptomyces viridochromogenes
98
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Streptomyces viridochromogenes
200
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Agrobacterium tumefaciens
201
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Streptomyces viridochromogenes
210
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Pseudomonas syringae pv. tomato
257
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Streptomyces viridochromogenes
269
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Fulvimarina pelagi
274
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Fulvimarina pelagi
342
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Agrobacterium tumefaciens
406
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Fulvimarina pelagi
430
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Fulvimarina pelagi
465
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Pseudomonas syringae pv. tomato
628
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Pseudomonas syringae pv. tomato
638
-
NAD+
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Streptomyces viridochromogenes
646
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Agrobacterium tumefaciens
785
-
NAD+
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Fulvimarina pelagi
794
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Pseudomonas syringae pv. tomato
829
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Agrobacterium tumefaciens
1083
-
NAD+
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Agrobacterium tumefaciens
1262
-
NAD+
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Pseudomonas syringae pv. tomato
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.03
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer; recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Oceanicola granulosus
0.04
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Oceanicola granulosus
0.07
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Oceanicola granulosus
0.1
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Oceanicola granulosus
0.11
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Oceanicola granulosus
0.3
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Pseudomonas syringae pv. tomato
0.7
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer; recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Streptomyces viridochromogenes
4
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Agrobacterium tumefaciens
9
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Pseudomonas syringae pv. tomato
11
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Agrobacterium tumefaciens
11
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Fulvimarina pelagi
12
-
D-Mannuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Fulvimarina pelagi
88
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Streptomyces viridochromogenes
98
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Streptomyces viridochromogenes
200
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Agrobacterium tumefaciens
201
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Streptomyces viridochromogenes
210
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Pseudomonas syringae pv. tomato
257
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Streptomyces viridochromogenes
269
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Fulvimarina pelagi
274
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Fulvimarina pelagi
342
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Agrobacterium tumefaciens
406
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Fulvimarina pelagi
430
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Fulvimarina pelagi
465
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Pseudomonas syringae pv. tomato
628
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Pseudomonas syringae pv. tomato
638
-
NAD+
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Streptomyces viridochromogenes
646
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Agrobacterium tumefaciens
785
-
NAD+
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Fulvimarina pelagi
794
-
beta-D-galacturonate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Pseudomonas syringae pv. tomato
829
-
beta-D-glucuronate
recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer
Agrobacterium tumefaciens
1083
-
NAD+
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Agrobacterium tumefaciens
1262
-
NAD+
recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer
Pseudomonas syringae pv. tomato
Other publictions for EC 1.1.1.203
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740393
Wagschal
Biochemical characterization o ...
Chromohalobacter salexigens, Polaromonas naphthalenivorans, Pseudomonas fluorescens, Pseudomonas fluorescens Pf0-1, Pseudomonas mendocina, Pseudomonas syringae pv. tomato, Pseudomonas syringae pv. tomato ATCC BAA-871 / DC3000
Enzyme Microb. Technol.
69
62-68
2015
-
-
5
1
-
-
3
25
-
-
-
14
-
10
-
-
5
-
-
-
-
-
14
5
5
-
-
14
5
-
-
10
1
-
-
-
-
5
10
1
-
-
-
3
1
25
-
-
-
14
-
-
-
5
-
-
-
-
14
5
5
-
-
14
5
-
-
-
-
5
5
-
15
15
740959
Pick
Characterization of uronate de ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens C58 / ATCC 33970, Fulvimarina pelagi, Oceanicola granulosus, Oceanicola granulosus DSM 15982, Pseudomonas syringae pv. tomato, Pseudomonas syringae pv. tomato DC3000, Streptomyces viridochromogenes, Streptomyces viridochromogenes DSM 40736
Microb. Biotechnol.
8
633-643
2015
-
-
5
-
-
-
-
40
-
-
-
18
-
19
-
-
-
-
-
-
-
-
36
-
5
5
5
35
5
5
-
10
-
-
-
-
-
5
10
-
-
-
-
-
-
40
-
-
-
18
-
-
-
-
-
-
-
-
36
-
5
5
5
35
5
5
-
-
-
10
10
-
32
32
743517
Xu
Expression profiling of genes ...
Raphanus sativus
Plant Physiol. Biochem.
70
269-277
2013
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
725435
Parkkinen
Crystal structure of uronate d ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens C58 / ATCC 33970
J. Biol. Chem.
286
27294-27300
2011
-
-
1
1
1
-
-
-
-
-
-
4
-
5
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
695552
Moon
Enzymatic assay of D-glucurona ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens C58 / ATCC 33970
Anal. Biochem.
392
183-185
2009
-
1
1
-
-
-
-
-
-
-
-
-
-
21
-
-
1
-
-
-
-
1
2
-
-
-
-
-
-
-
-
1
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
695783
Moon
Production of glucaric acid fr ...
Pseudomonas syringae pv. tomato, Pseudomonas syringae pv. tomato DC3000
Appl. Environ. Microbiol.
75
589-595
2009
-
-
1
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
1
-
4
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
698607
Yoon
Cloning and characterization o ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens C58 / ATCC 33970, Pseudomonas putida KT2440, Pseudomonas syringae pv. tomato, Pseudomonas syringae pv. tomato DC3000
J. Bacteriol.
191
1565-1573
2009
-
-
3
-
-
-
-
9
-
-
3
-
-
17
-
-
3
-
-
-
-
-
10
3
-
-
-
6
3
-
-
3
-
-
-
-
-
3
3
-
-
-
-
-
-
9
-
-
3
-
-
-
-
3
-
-
-
-
10
3
-
-
-
6
3
-
-
-
-
-
-
-
-
-
286231
Wagner
Uronic acid dehydrogenase from ...
Pseudomonas syringae
Eur. J. Biochem.
61
589-596
1976
-
-
-
-
-
1
4
3
-
-
2
2
-
2
-
-
1
-
1
1
1
2
3
1
1
-
3
2
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
4
-
3
-
-
2
2
-
-
-
1
1
1
1
2
3
1
1
-
3
2
-
-
-
-
-
-
-
-
-
-
286230
Bateman
Purification and properties of ...
Pseudomonas syringae
Arch. Biochem. Biophys.
136
97-105
1970
-
-
-
-
-
1
1
3
-
-
-
2
-
2
-
-
1
-
-
1
1
1
2
-
-
-
2
-
1
-
2
1
-
-
-
-
-
-
1
-
-
1
-
1
-
3
-
-
-
2
-
-
-
1
-
1
1
1
2
-
-
-
2
-
1
-
2
-
-
-
-
-
-
-