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Search Purification (Commentary)

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EC Number Purification (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.7- 637845, 637846, 637847, 637849, 637850, 637852, 637854, 679550, 679829, 680980, 687853, 756927, 758280
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.76 × His-tagged recombinant enzyme is purified by Ni-NTA affinity chromatography 758286
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.7less than 10% impurities 637843
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.7mutant enzymes D295N and D295E 679850
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.7native enzyme 10fold, partially by gel filtration 704230
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.7partial 637842
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.7purification of thermostable mutant T47S/S62P/Y77H/V128L/K140M/Q144R/N155S/D249G by heating at 65°C for 20 min with 20% sucrose. The purified enzyme can be directly employed for the production of amylose with further purification 680405
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.7purified using standard immobilised metal affinity chromatography, facilitated by the fused 6-histidine tag 757282
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.7recombinant enzyme from Escherichia coli 657623
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.7recombinant enzyme from Escherichia coli by anion exchange and hydrophobic interaction chromatography 685688
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