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Literature summary for 2.4.1.7 extracted from
- Expression and characterization of recombinant sucrose phosphorylase (2018), Protein J., 37, 93-100 .
Application
| Application | Comment | Organism |
|---|---|---|
| industry | the enzyme is widely used in the food, cosmetics, and pharmaceutical industries | Bifidobacterium longum |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21. Expression conditions are optimized. The highest expression efficiency is obtained at an initial cell density of OD600 0.5, with 0.05 mM isopropyl-beta-D-thiogalactoside, followed by shaking at 180 rpm and incubation at 30°C for 15 h | Bifidobacterium longum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56510 | - |
SDS-PAGE, calculated from sequence | Bifidobacterium longum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bifidobacterium longum | A5A8M5 | - |
- |
| Bifidobacterium longum JCM1217 | A5A8M5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 6 × His-tagged recombinant enzyme is purified by Ni-NTA affinity chromatography | Bifidobacterium longum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 122.1 | - |
pH and temperature not specified in the publication | Bifidobacterium longum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SPase | - |
Bifidobacterium longum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Bifidobacterium longum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 90 | 20°C: about 55% of maximal activity, 90°C: about 80% of maximal activity | Bifidobacterium longum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 55 | 1 h, the enzyme retains more than 80% relative activity at 30-55°C | Bifidobacterium longum |
| 60 | 90 | 1 h, the enzyme retains more than 70% relative activity at 60-90°C | Bifidobacterium longum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Bifidobacterium longum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 7 | pH 5.5: about 45% of maximal activity, pH 7.0: about 55% of maximal activity | Bifidobacterium longum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
45°C, 1 h, 80% loss of activity | Bifidobacterium longum |
| 6 | 6.5 | 45°C, 1 h, stable | Bifidobacterium longum |
| 7 | - |
45°C, 1 h, 25% loss of activity | Bifidobacterium longum |
| 7.5 | - |
45°C, 1 h, 60% loss of activity | Bifidobacterium longum |
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Release 2023.1 (January 2023)
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