Literature summary for 2.4.1.7 extracted from

Li, Y.; Li, Z.; He, X.; Chen, L.; Cheng, Y.; Jia, H.; Yan, M.; Chen, K.
Characterisation of a Thermobacillus sucrose phosphorylase and its utility in enzymatic synthesis of 2-O-beta-D-glucopyranosyl-L-ascorbic acid (2019), J. Biotechnol., 305, 27-34 .

Search for more literature for 2.4.1.7

Application

Application	Comment	Organism
synthesis	the enzyme is a promising biocatalyst for the glycosylation of a wide variety of carbohydrates and non-carbohydrate molecules. It is important for enzymatic synthesis of alpha-D-glycosides, which are widely used in food, medicine, cosmetics and other industries. It generally reversibly catalyses the conversion of sucrose and phosphate into fructose and alpha-D-glucose-1-phosphate, and it can also transfer glucosyl groups from sucrose to a hydroxy group in a suitable acceptor, generating new alpha-D-glucosidic products such as ascorbic acid 2-glucoside, alpha-arbutin, 2-O-(alpha-D-glucopyranosyl)-sn-glycerol. 2-O-alpha-D-glucopyranosyl-L-ascorbic acid is produced after incubation of ascorbic acid sodium salt and sucrose (1:2) with 19.76 U/ml of the enzyme at pH 7.0 and 50°C for 24 h, with a maximum yield of 19.7% (39.94 g/l)	Thermobacillus sp. ZCTH02-B1

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli BL21	Thermobacillus sp. ZCTH02-B1

Inhibitors

Inhibitors	Comment	Organism	Structure
Ca2+	5 mM, 11% loss of activity	Thermobacillus sp. ZCTH02-B1
Cu2+	5 mM, 13% loss of activity	Thermobacillus sp. ZCTH02-B1
EDTA	5 mM, 15% loss of activity	Thermobacillus sp. ZCTH02-B1
Fe2+	5 mM, 19% loss of activity	Thermobacillus sp. ZCTH02-B1
Li+	5 mM, 15% loss of activity	Thermobacillus sp. ZCTH02-B1
Mg2+	5 mM, 5% loss of activity	Thermobacillus sp. ZCTH02-B1
Mn2+	5 mM, 5% loss of activity	Thermobacillus sp. ZCTH02-B1
Na+	5 mM, 11% loss of activity	Thermobacillus sp. ZCTH02-B1

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
6.24	-	sucrose	pH 7.0, 55°C	Thermobacillus sp. ZCTH02-B1

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	5 mM, 17% activation	Thermobacillus sp. ZCTH02-B1

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
55800	-	calculated from sequence	Thermobacillus sp. ZCTH02-B1

Organic Solvent Stability

Organic Solvent	Comment	Organism
DMSO	the enzyme displays a high tolerance towards DMSO	Thermobacillus sp. ZCTH02-B1

Organism

Organism	UniProt	Comment	Textmining
Thermobacillus sp. ZCTH02-B1	A0A1Y3Q6Q6	-	-

Purification (Commentary)

Purification (Comment)	Organism
purified using standard immobilised metal affinity chromatography, facilitated by the fused 6-histidine tag	Thermobacillus sp. ZCTH02-B1

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
6.42	-	pH 7.0, 55°C	Thermobacillus sp. ZCTH02-B1

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
sucrose + L-ascorbic acid	-	Thermobacillus sp. ZCTH02-B1	D-fructose + 2-O-alpha-D-glucopyranosyl-L-ascorbic acid	-	?
sucrose + phosphate	-	Thermobacillus sp. ZCTH02-B1	D-fructose + alpha-D-glucose 1-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
SPase	-	Thermobacillus sp. ZCTH02-B1

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
65	-	-	Thermobacillus sp. ZCTH02-B1

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
50	-	t1/2: 212 h	Thermobacillus sp. ZCTH02-B1
60	-	t1/2: 98 h	Thermobacillus sp. ZCTH02-B1

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
5.73	-	sucrose	pH 7.0, 55°C	Thermobacillus sp. ZCTH02-B1

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Thermobacillus sp. ZCTH02-B1

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
5	8	greater than 80% of its original activity is retained when the enzyme is incubated at pH 5.0-8.0 for 1 h	Thermobacillus sp. ZCTH02-B1
7	-	most stable at pH 7.0	Thermobacillus sp. ZCTH02-B1
9	-	less than 50% of the activity remains when the enzyme is incubated at pH 9.0 for 1 h	Thermobacillus sp. ZCTH02-B1

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Release 2023.1 (January 2023)