Cloned (Comment) | Organism |
---|---|
gene sucP, cloning from genomic library, DNA and amino acid sequence determination and analysis, determination of promotor region, expression in Escherichia coli | Bifidobacterium adolescentis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Bifidobacterium adolescentis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56189 | - |
2 * 58000, SDS-PAGE, 2 * 56189, sequence calculation | Bifidobacterium adolescentis |
58000 | - |
2 * 58000, SDS-PAGE, 2 * 56189, sequence calculation | Bifidobacterium adolescentis |
129000 | - |
gel filtration | Bifidobacterium adolescentis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + phosphate | Bifidobacterium adolescentis | - |
beta-D-fructose + alpha-D-glucose 1-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bifidobacterium adolescentis | Q84HQ2 | DSM20083, gene sucP | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli | Bifidobacterium adolescentis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
109 | - |
purified recombinant enzyme, substrate sucrose | Bifidobacterium adolescentis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-arabinose + alpha-D-glucose 1-phosphate | - |
Bifidobacterium adolescentis | alpha-D-Glc(1-1)-beta-D-Ara + phosphate | - |
? | |
D-arabitol + alpha-D-glucose 1-phosphate | - |
Bifidobacterium adolescentis | ? + phosphate | - |
? | |
D-fructose + alpha-D-glucose 1-phosphate | low activity | Bifidobacterium adolescentis | ? + phosphate | - |
? | |
D-fucose + alpha-D-glucose 1-phosphate | low activity | Bifidobacterium adolescentis | ? + phosphate | - |
? | |
D-galactose + alpha-D-glucose 1-phosphate | low activity | Bifidobacterium adolescentis | ? + phosphate | - |
? | |
D-glucose + alpha-D-glucose 1-phosphate | low activity | Bifidobacterium adolescentis | ? + phosphate | - |
? | |
D-sorbitol + alpha-D-glucose 1-phosphate | - |
Bifidobacterium adolescentis | ? + phosphate | - |
? | |
D-xylitol + alpha-D-glucose 1-phosphate | high activity | Bifidobacterium adolescentis | ? + phosphate | - |
? | |
D-xylose + alpha-D-glucose 1-phosphate | low activity | Bifidobacterium adolescentis | ? + phosphate | - |
? | |
L-arabinose + alpha-D-glucose 1-phosphate | high activity | Bifidobacterium adolescentis | ? + phosphate | - |
? | |
L-arabitol + alpha-D-glucose 1-phosphate | high activity | Bifidobacterium adolescentis | ? + phosphate | - |
? | |
L-fucose + alpha-D-glucose 1-phosphate | - |
Bifidobacterium adolescentis | ? + phosphate | - |
? | |
L-sorbose + alpha-D-glucose 1-phosphate | - |
Bifidobacterium adolescentis | ? + phosphate | - |
? | |
L-xylose + alpha-D-glucose 1-phosphate | low activity | Bifidobacterium adolescentis | ? + phosphate | - |
? | |
additional information | substrate specificity, di- and trisaccharides, including sucrose, are no acceptor substrate, overview | Bifidobacterium adolescentis | ? | - |
? | |
sucrose + phosphate | - |
Bifidobacterium adolescentis | beta-D-fructose + alpha-D-glucose 1-phosphate | - |
r | |
sucrose + phosphate | enzyme prefers the forward reaction | Bifidobacterium adolescentis | beta-D-fructose + alpha-D-glucose 1-phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 58000, SDS-PAGE, 2 * 56189, sequence calculation | Bifidobacterium adolescentis |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to the glycoside hydrolase family 13 | Bifidobacterium adolescentis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
48 | - |
- |
Bifidobacterium adolescentis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 6.5 | - |
Bifidobacterium adolescentis |