EC Number   |
Posttranslational Modification |
Reference |
|---|
   3.1.3.53 | phosphoprotein |
phosphorylation of MYPT1 by Rho-associated coiled-coil kinase ROCK occurs at residues Thr697 and Thr855. cAMP-dependent protein kinase phosphorylates these two sites and the neighboring Ser696 and Ser854. Prior phosphorylation at Thr697 and Thr855 by ROCK precludes phosphorylation at Ser696 and Ser854 by cAMP-dependent protein kinase. Phosphorylation at Thr697 and Thr855 by ROCK exposes two other sites of phosphorylation by cAMP-dependent protein kinase |
749533 |
| 3.1.3.53 | phosphoprotein |
phosphorylation of MYPT1 is a major mechanism of MLCP regulation. Two major ROK phosphorylation sites, Thr697 and Thr855, on MYPT1 elicit MLCP inhibition. Phosphorylation of both sites inhibits MLCP activity. MYPT1-T855 phosphorylation may also interfere with the binding of MYPT1 to myosin |
713963 |
| 3.1.3.53 | phosphoprotein |
phosphorylation of MYPT1 residue T694 is a primary mechanism contributing to inhibition of myosin light chain phosphatase activity and enhancement of myosin regulatory light chain phosphorylation in vivo |
751394 |
| 3.1.3.53 | phosphoprotein |
phosphorylation of the myosin-binding subunit of MLCP at the inhibitory site Thr-641 inhibits MLCP activity, but may not play a significant role in a mechanism for MLCP regulation, Rho kinase phosphorylates Thr-799, but not Thr-641 |
646366 |
| 3.1.3.53 | phosphoprotein |
regulatory subunit of enzmye is phopshorylated at T696, contraction of intact mouse arteries induced with noradrenaline is associated with a 2fold increase in phosphorylation, which is reversed in arteries relaxed with urocortin |
664611 |
| 3.1.3.53 | phosphoprotein |
selective thiophosphorylation of MYPT1 at Thr696 with ROCK inhibits the MLCP activity 30%, whereas the Thr853 thio-phosphorylation does not alter the phosphatase activity. Both Thr696 and Thr853 sites undergo autodephosphorylation |
731315 |
| 3.1.3.53 | phosphoprotein |
Thr-654 and Thr-695 are the major phosphorylation sites of the large subunit MYPT isoforms M130 and M133 |
646371 |
| 3.1.3.53 | phosphoprotein |
treatment of Triton-skinned rat caudal arterial smooth muscle strips with the membrane-impermeant phosphatase inhibitor microcystin or treatment of intact tissue with the membrane-permeant phosphatase inhibitor calyculin A induces slow, sustained contractions that correlate with phosphorylation of MYPT1 at 7 to more than 10 sites |
749533 |
| 3.1.3.53 | proteolytic modification |
in apoptotic cells, the myosin-binding domain of myosin phosphatase targeting subunit 1 (MYPT1) is cleaved by caspase-3 at Asp-884 |
732482 |
