EC Number   |
Application |
Reference |
|---|
   4.2.3.73 | synthesis |
8fold improvement in the production of valencene, in yeast co-engineered with a truncated and deregulated HMG1, mitochondrion-targeted heterologous farnesyl diphosphate synthase and a mitochondrion-targeted sesquiterpene synthase,i.e.valencene synthase. Production of the Citrus sesquiterpene valencene in yeast is affected by deletion of geranylgeranyl diphosphate synthase BTS1 but not of phosphatases DPP1 or LPP1 |
712936 |
| 4.2.3.73 | synthesis |
expression of valencene synthase in Saccharomyces cerevisiae indicates potential for higher yields. In an optimized Rhodobacter sphaeroides strain, expression of valencene synthase increases valencene yields 14fold to 352 mg/l |
730580 |
| 4.2.3.73 | synthesis |
heterologous expression of the (+)-valencene synthase gene in Corynebacterium glutamicum is not sufficient to enable (+)-valencene production, likely because provision of farnesyl diphosphate by endogenous prenyltransferases is too low. Upon deletion of two endogenous prenyltransferase genes and heterologous expression of either farnesyl diphosphate synthase gene ispA from Escherichia coli or ERG20 from Saccharomyces cerevisiae (+)-valencene production is observed. n-Dodecane is suitable for extraction of (+)-valencene from cultures and compatible with growth of Corynabacterium glutamicum. Production based on (+)-valencene synthase from Nootka cypress is superior to production by the enzyme from Citrus sinensis |
730098 |
| 4.2.3.73 | synthesis |
Introduction of a valencene synthase gene into mushroom-forming fungus Schizophyllum commune results in production of the sesquiterpene (+)-valencene, both in mycelium and in fruiting bodies. Levels of (+)-valencene in culture media of strains containing a mutated RGS regulatory protein gene are increased fourfold compared to those in wild-type transformants. Up to 16 mg l/1 (+)-valencene can be produced. The amount of (+)-valencene containing n-dodecane recovered from the culture medium increases six- to sevenfold in the mutant strains due to the absence of schizophyllan |
729081 |
