Literature summary for 4.2.3.73 extracted from

Frohwitter, J.; Heider, S.; Peters-Wendisch, P.; Beekwilder, J.; Wendisch, V.
Production of the sesquiterpene (+)-valencene by metabolically engineered Corynebacterium glutamicum (2014), J. Biotechnol., 191, 205-213.

Search for more literature for 4.2.3.73

Application

Application	Comment	Organism
synthesis	heterologous expression of the (+)-valencene synthase gene in Corynebacterium glutamicum is not sufficient to enable (+)-valencene production, likely because provision of farnesyl diphosphate by endogenous prenyltransferases is too low. Upon deletion of two endogenous prenyltransferase genes and heterologous expression of either farnesyl diphosphate synthase gene ispA from Escherichia coli or ERG20 from Saccharomyces cerevisiae (+)-valencene production is observed. n-Dodecane is suitable for extraction of (+)-valencene from cultures and compatible with growth of Corynabacterium glutamicum. Production based on (+)-valencene synthase from Nootka cypress is superior to production by the enzyme from Citrus sinensis	Citrus sinensis
synthesis	heterologous expression of the (+)-valencene synthase gene in Corynebacterium glutamicum is not sufficient to enable (+)-valencene production, likely because provision of farnesyl diphosphate by endogenous prenyltransferases is too low. Upon deletion of two endogenous prenyltransferase genes and heterologous expression of either farnesyl diphosphate synthase gene ispA from Escherichia coli or ERG20 from Saccharomyces cerevisiae (+)-valencene production is observed. n-Dodecane is suitable for extraction of (+)-valencene from cultures and compatible with growth of Corynabacterium glutamicum. Production based on (+)-valencene synthase from Nootka cypress is superior to production by the enzyme from Citrus sinensis	Callitropsis nootkatensis

Organism

Organism	UniProt	Comment	Textmining
Callitropsis nootkatensis	V9N2L5	-	-
Citrus sinensis	Q71MJ3	-	-

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)