Literature summary for 4.2.3.73 extracted from

Beekwilder, J.; van Houwelingen, A.; Cankar, K.; van Dijk, A.D.; de Jong, R.M.; Stoopen, G.; Bouwmeester, H.; Achkar, J.; Sonke, T.; Bosch, D.
Valencene synthase from the heartwood of Nootka cypress (Callitropsis nootkatensis) for biotechnological production of valencene (2014), Plant Biotechnol. J., 12, 174-182.

Search for more literature for 4.2.3.73

Application

Application	Comment	Organism
synthesis	expression of valencene synthase in Saccharomyces cerevisiae indicates potential for higher yields. In an optimized Rhodobacter sphaeroides strain, expression of valencene synthase increases valencene yields 14fold to 352 mg/l	Callitropsis nootkatensis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00104	-	(2E,6E)-farnesyl diphosphate	pH 7.0, 30°C	Callitropsis nootkatensis

Organism

Organism	UniProt	Comment	Textmining
Callitropsis nootkatensis	V9N2L5	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	expression in different tissues from the tree correlates well with nootkatone content	Callitropsis nootkatensis	-
stem	wood bottom stem and top stem, high expression	Callitropsis nootkatensis	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(2E,6E)-farnesyl diphosphate	-	Callitropsis nootkatensis	(+)-valencene + diphosphate	plus minor product germacrene A. At pH 7, germacrene A accounts for approximately 6% of the total sesquiterpenes produced. With increasing pH, the amount of side-product germacrene A also increases	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	-	Callitropsis nootkatensis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0032	-	(2E,6E)-farnesyl diphosphate	pH 7.0, 30°C	Callitropsis nootkatensis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Callitropsis nootkatensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
3.4	-	(2E,6E)-farnesyl diphosphate	pH 7.0, 30°C	Callitropsis nootkatensis

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)