EC Number   |
Application |
Reference |
|---|
    4.2.1.84 | analysis |
the enzyme is used in production of acrylamide from acrylonitrile. For maximum production of Co2+ containing nitrile hydratase, is cultured in the medium containing lactose (18.0 g/l), peptone (1.0 g/l), yeast extract (2.0 g/l), MgSO4 (0.5 g/l), K2HPO4 (0.6 g/l), urea (9.0 g/l), and CoCl2 (0.01 g/l), pH 7.0, and incubated at 35°C for 24 h in an incubator shaker (160 rpm). Nitrile hydratase exhibits relatively high specificity for aliphatic nitriles. Free cells are immobilized using 2% (w/v) agar solution to enhance enzyme stability and reusability in repetitive cycles of acrylamide production. Under optimized conditions, nearly complete bioconversion of acrylonitrile is achieved with a fair recovery of 85% using free and immobilized cells equivalent to 500 mg/dry cell weight/l |
746567 |
| 4.2.1.84 | degradation |
treatment of acetonitrile-containing wastes on-site, Brevundimonas diminuta containing enzyme degrades acetonitrile at concentrations up to 6 M |
-, 663744 |
| 4.2.1.84 | degradation |
treatment of acetonitrile-containing wastes on-site, Rhodococcus pyridinivorans S85-2 containing enzyme degrades acetonitrile at concentrations up to 6 M |
-, 663744 |
| 4.2.1.84 | environmental protection |
degradation of nitrile waste |
-, 676087 |
| 4.2.1.84 | environmental protection |
NHase is used in two-step degradation (including amidase, EC 3.5.1.4) of acetonitrile-containing waste |
-, 671557 |
| 4.2.1.84 | industry |
biotransformation of nitrile. Nitrile hydratase from Rhodococcus rhodochrous J1 is used for industrial production of acrylamide and nicotinamide. Production of enzyme by recombinant Escherichia coli is superior to that in R. rhodochrous J1. Genetically engineered Escherichia coli can be used for industrial applications instead of Rhodococcus rhodochrous J1. High-molecular weight nitrile hydratase may be more suitable for industrial application than low-molecular weight nitrile hydratase because of its higher product tolerance, which would lead to a high product concentration |
-, 749108 |
| 4.2.1.84 | more |
Rhodococcus erythropolis A4 converts benzonitrile herbicides into amides, the strain is able to hydrolyze 2,6-dichlorobenzamide into 2,6-dichlorobenzoic acid, and produces also the carboxylic acids from the other herbicides. Transformation of nitriles into amides decreases acute toxicities for chloroxynil and dichlobenil, but increases them for bromoxynil and ioxynil. The amides inhibit root growth in Lactuca sativa less than the nitriles but more than the acids. The conversion of the nitrile group may be the first step in the mineralization of benzonitrile herbicides but cannot be itself considered to be a detoxification |
-, 730132 |
| 4.2.1.84 | more |
Rhodococcus rhodochrous PA-34 converts benzonitrile herbicides into amides, but the strain does not hydrolyze 2,6-dichlorobenzamide into 2,6-dichlorobenzoic acid. Transformation of nitriles into amides decreases acute toxicities for chloroxynil and dichlobenil, but increases them for bromoxynil and ioxynil. The amides inhibit root growth in Lactuca sativa less than the nitriles but more than the acids. The conversion of the nitrile group may be the first step in the mineralization of benzonitrile herbicides but cannot be itself considered to be a detoxification |
-, 730132 |
| 4.2.1.84 | pharmacology |
synthesis, biotransformation and biocatalysis of unsaturated/saturated aliphatic, aromatic and heterocyclic nitriles |
-, 677265 |
| 4.2.1.84 | synthesis |
- |
-, 648433, 648438, 648440, 648441 |
